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Your search keyword '"Megan M. Kibbey"' showing total 2 results
Start Over Author "Megan M. Kibbey" Topic biophysics
2 results on '"Megan M. Kibbey"'

1. IGF-1 induced vascular endothelial growth factor secretion in head and neck squamous cell carcinoma

Author

Leigh Ann Black, Steven A. Rosenzweig, Megan M. Kibbey, Mark G. Slomiany, and Terry A. Day

Subjects
Transcriptional Activation, Vascular Endothelial Growth Factor A, MAPK/ERK pathway, medicine.medical_specialty, medicine.drug_class, Cell, Biophysics, Models, Biological, Biochemistry, Tyrosine-kinase inhibitor, Receptor, IGF Type 1, Phosphatidylinositol 3-Kinases, chemistry.chemical_compound, Internal medicine, medicine, Humans, Secretion, Insulin-Like Growth Factor I, Autocrine signalling, Molecular Biology, Protein kinase B, Cell Cycle, Cobalt, Cell Biology, Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, Head and neck squamous-cell carcinoma, Vascular endothelial growth factor, Autocrine Communication, Receptors, Vascular Endothelial Growth Factor, Endocrinology, medicine.anatomical_structure, chemistry, Head and Neck Neoplasms, Culture Media, Conditioned, Carcinoma, Squamous Cell, Cancer research, Hypoxia-Inducible Factor 1, Mitogen-Activated Protein Kinases, Signal Transduction
Abstract

Elevated vascular endothelial growth factor (VEGF) levels correlate with increased progression and poor prognosis of head and neck squamous cell carcinomas (HNSCC). VEGF expression is regulated by hypoxia and cytokines, including insulin-like growth factor-1 (IGF-1). In this report, we examined IGF-1 signaling and VEGF expression in SCC-9 cells. IGF-1 and the chemical hypoxia agent, cobalt chloride, each stimulated VEGF secretion and VEGF promoter activation. Cobalt chloride increased Hif-1α protein levels and HIF-1 dependent activation of the enolase promoter. IGF-1 increased these parameters only in the presence of cobalt chloride. IGF-1 stimulated PI-3K/Akt and Erk/MAPK pathways in SCC-9 cells, each contributing to Hif-1α expression and VEGF secretion. SCC-9 cells express the VEGF receptors Flk-1 and neuropilin-1, with VEGF treatment increasing VEGF promoter activity and VEGF secretion that was attenuated by the Flk-1 tyrosine kinase inhibitor, ZM 323881. These results demonstrate the presence of an IGF-1 regulated VEGF autocrine loop in HNSCC.

Published
2006
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2. Spontaneous and reversible self-assembly of a polypeptide fragment of insulin-like growth factor binding protein-2 into fluorescent nanotubular structures†

Author

Hanudatta S. Atreya, Monalisa Swain, Bankala Krishnarjuna, Megan M. Kibbey, Erin M. Eaton, Ravula Thirupathi, and Steven A. Rosenzweig

Subjects
Protein Folding, Magnetic Resonance Spectroscopy, medicine.medical_treatment, Catalysis, Insulin-like growth factor-binding protein, Article, Materials Chemistry, medicine, Fluorescent Dyes, Nanotubes, biology, Chemistry, Binding protein, Growth factor, Metals and Alloys, General Chemistry, Nuclear magnetic resonance spectroscopy, Fluorescence, In vitro, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Insulin-Like Growth Factor Binding Protein 2, Biochemistry, Amino Acid Substitution, Microscopy, Fluorescence, Ceramics and Composites, Biophysics, biology.protein, Mutagenesis, Site-Directed, Protein folding, Self-assembly
Abstract

In this communication, we report the spontaneous and reversible in vitro self-assembly of a polypeptide fragment derived from the C-terminal domain of Insulin-like Growth Factor Binding Protein (IGFBP-2) into soluble nanotubular structures several micrometres long via a mechanism involving inter-molecular disulfide bonds and exhibiting enhanced fluorescence.

Published
2009

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