16 results on '"Almeida, C. Marisa R."'
Search Results
2. Atlas of the microbial degradation of fluorinated pesticides.
- Author
-
Alexandrino, Diogo A. M., Mucha, Ana P., Almeida, C. Marisa R., and Carvalho, Maria F.
- Subjects
PESTICIDES ,PESTICIDE pollution ,MICROBIAL metabolism ,AGRICULTURAL chemicals ,PHENYL ethers ,AROMATIC fluorine compounds - Abstract
Fluorine-based agrochemicals have been benchmarked as the golden standard in pesticide development, prompting their widespread use in agriculture. As a result, fluorinated pesticides can now be found in the environment, entailing serious ecological implications due to their harmfulness and persistence. Microbial degradation might be an option to mitigate these impacts, though environmental microorganisms are not expected to easily cope with these fluoroaromatics due to their recalcitrance. Here, we provide an outlook on the microbial metabolism of fluorinated pesticides by analyzing the degradation pathways and biochemical processes involved, while also highlighting the central role of enzymatic defluorination in their productive metabolism. Finally, the potential contribution of these microbial processes for the dissipation of fluorinated pesticides from the environment is also discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
3. Microbial response to a port fuel spill: Community dynamics and potential for bioremediation.
- Author
-
Perdigão, Rafaela, Tomasino, Maria Paola, Magalhães, Catarina, Carvalho, Maria F., Almeida, C. Marisa R., and Mucha, Ana P.
- Subjects
BIOREMEDIATION ,RHODOCOCCUS erythropolis ,OIL spills ,MICROBIAL communities ,GENETIC barcoding - Abstract
Following a fuel leakage inside a Portuguese maritime port, we conducted parallel 30-day experiments using contaminated seawater and fuel, sampled five days after the incident. This study aimed to (i)survey the native microbial community response to the spilled fuel and (ii)evaluate the efficacy of bioremediation, both biostimulation and bioaugmentation with a lyophilized bacterial consortium (Rhodococcus erythropolis , Pseudomonas sp.), in accelerating hydrocarbon degradation. Metabarcoding analysis revealed a shift in microbial communities, with increased abundance of hydrocarbon-degraders (e.g. Alcanivorax , Thalassospira). Ninety-five hydrocarbonoclastic bacteria were isolated, including key groups from the enriched communities. The lyophilized bacteria added in bioaugmentation, enhanced the abundance of hydrocarbon-degraders over time and were recovered throughout time. Bioremediation treatments favoured biodegradation, achieving over 60 % removal of total petroleum hydrocarbons after 15 days, contrasting with natural attenuation where almost no TPH was removed. This work highlights the potential of bioremediation technologies to accelerate hydrocarbon-degrading activity, for oil spills inside ports. [Display omitted] • Spilled fuel and seawater from a port were used for enrichment experiments. • Enriched communities were dominated by Proteobacteria and Bacteroidota members. • Lyophilized bacterial consortium enhanced the abundance of HC-degraders early on. • Bioremediation treatments favoured the biodegradation process (>60 % TPH removal). [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. Potential of the microbial community present in an unimpacted beach sediment to remediate petroleum hydrocarbons
- Author
-
Almeida, C. Marisa R., Reis, Izabela, Couto, M. Nazaré, Bordalo, Adriano. A., and Mucha, Ana P.
- Published
- 2013
- Full Text
- View/download PDF
5. Hydrocarbon degradation potential of salt marsh plant–microorganisms associations
- Author
-
Ribeiro, Hugo, Mucha, Ana P., Almeida, C. Marisa R., and Bordalo, Adriano A.
- Published
- 2011
- Full Text
- View/download PDF
6. Harnessing the Potential of Native Microbial Communities for Bioremediation of Oil Spills in the Iberian Peninsula NW Coast.
- Author
-
Bôto, Maria L., Magalhães, Catarina, Perdigão, Rafaela, Alexandrino, Diogo A. M., Fernandes, Joana P., Bernabeu, Ana M., Ramos, Sandra, Carvalho, Maria F., Semedo, Miguel, LaRoche, Julie, Almeida, C. Marisa R., and Mucha, Ana P.
- Subjects
MICROBIAL remediation ,OIL spills ,POLYCYCLIC aromatic compounds ,BIOTIC communities ,RESTORATION ecology ,MICROBIAL communities ,MARINE debris - Abstract
Oil spills are among the most catastrophic events to marine ecosystems and current remediation techniques are not suitable for ecological restoration. Bioremediation approaches can take advantage of the activity of microorganisms with biodegradation capacity thus helping to accelerate the recovery of contaminated environments. The use of native microorganisms can increase the bioremediation efficiency since they have higher potential to survive in the natural environment while preventing unpredictable ecological impacts associated with the introduction of non-native organisms. In order to know the geographical scale to which a native bioremediation consortium can be applied, we need to understand the spatial heterogeneity of the natural microbial communities with potential for hydrocarbon degradation. In the present study, we aim to describe the genetic diversity and the potential of native microbial communities to degrade petroleum hydrocarbons, at an early stage of bioremediation, along the NW Iberian Peninsula coast, an area particularly susceptible to oil spills. Seawater samples collected in 47 sites were exposed to crude oil for 2 weeks, in enrichment experiments. Seawater samples collected in situ , and samples collected after the enrichment with crude oil, were characterized for prokaryotic communities by using 16S rRNA gene amplicon sequencing and predictive functional profiling. Results showed a drastic decrease in richness and diversity of microbial communities after the enrichment with crude oil. Enriched microbial communities were mainly dominated by genera known to degrade hydrocarbons, namely Alcanivorax , Pseudomonas , Acinetobacter , Rhodococcus , Flavobacterium , Oleibacter , Marinobacter , and Thalassospira , without significant differences between geographic areas and locations. Predictive functional profiling of the enriched microbial consortia showed a high potential to degrade the aromatic compounds aminobenzoate, benzoate, chlorocyclohexane, chlorobenzene, ethylbenzene, naphthalene, polycyclic aromatic compounds, styrene, toluene, and xylene. Only a few genera contributed for more than 50% of this genetic potential for aromatic compounds degradation in the enriched communities, namely Alcanivorax , Thalassospira , and Pseudomonas spp. This work is a starting point for the future development of prototype consortia of hydrocarbon-degrading bacteria to mitigate oil spills in the Iberian NW coast. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
7. Bioremediation of bezafibrate and paroxetine by microorganisms from estuarine sediment and activated sludge of an associated wastewater treatment plant.
- Author
-
Duarte, Patrícia, Almeida, C. Marisa R., Fernandes, Joana P., Morais, Daniela, Lino, Marta, Gomes, Carlos R., Carvalho, Maria F., and Mucha, Ana P.
- Abstract
Abstract The present work aimed to explore the potential of autochthonous microorganisms from an urban estuary and from activated sludge of an associated wastewater treatment plant (WWTP), for biodegradation of an antidepressant drug, paroxetine, and on a cholesterol-lowering agent, bezafibrate. These compounds were chosen as representatives of extensively used pharmaceuticals. Autochthonous microorganisms from the indicated sources were exposed to the target pharmaceuticals (1 mg/L) in co-metabolism with sodium acetate (500 mg/L) along a two-weeks period, for a total of 7 two-weeks periods (here referred as cycles). Exposures were carried out in batch mode, under different incubation conditions (agitation vs. static). Removal of pharmaceuticals was monitored at the end of each cycle, by analysing the culture medium. For paroxetine, fluoride ion release was also followed as an indicator of defluorination of the molecule. The structure of the bacterial communities was analysed by ARISA (Automated rRNA Intergenic Spacer Analysis), at the beginning of the experiment and at the end of the first and the last cycles to identify substantial changes associated with the time of exposure, the incubation conditions and the presence and type of pharmaceuticals. Incubation conditions affected not only the bacterial community structure, but also the biodegradation efficiency. At the beginning of the experiment, removal of target pharmaceuticals was found to be lower under agitation than under static conditions, but at the end of the experiment, results showed high removal of the pharmaceuticals from the culture medium (>97%) under both conditions, mainly by microbiological processes. For paroxetine, adsorption and abiotic processes also had an important influence on its removal, but defluorination only occurred in the presence of microorganisms. These results highlight that autochthonous microorganisms from estuarine sediments and WWTP sludge have high ability to remove the selected pharmaceuticals with relevant implications for the development of new bioremediation tools for environmental restoration. Graphical abstract Unlabelled Image Highlights • Potential of autochthonous microorganisms for bioremediation of pharmaceuticals tested • Microorganisms from a sediment and an activated sludge degraded paroxetine and bezafibrate • Microbial community structure changed to adapt to pharmaceuticals presence • Results are relevant for development of new bioremediation tools for environmental restoration. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
8. Buried and surface oil degradation – Evaluating bioremediation to increase PAHs removal through linear mathematical models.
- Author
-
Stoichev, Teodor, Mucha, Ana P., Bernabeu, Ana, and Almeida, C. Marisa R.
- Subjects
BIOREMEDIATION ,POLYCYCLIC aromatic hydrocarbons ,ADDITIVE functions ,MATHEMATICAL models ,PETROLEUM ,BIOSURFACTANTS - Abstract
A bioremediation approach with tide simulation for buried and surface oil degradation was tested for removal of two, three and four rings polycyclic aromatic hydrocarbons (PAHs). Linear models depicted degradation constants of individual PAH as simple additive function of their initial concentrations (C 0) in contaminated sand, hydrophobicity, sampling layer and treatment conditions. For all PAHs and treatment conditions, the degradation of oil in buried layers was faster than at the surface. Naturally-occurring microorganisms proved to be efficient for bioremediation of PAHs and were stimulated by fertilizer addition (biostimulation, BS). Bioaugmentation (BA) by addition of a slurry of a native oil-degraders pre-stimulated consortium did not show faster PAH degradation than BS. Degradation was more rapid for PAH present at low C 0 and with intermediate hydrophobicity. Bioremediation of beach sand either with surface or buried crude oil is a cost-effective strategy to clean-up different hydrocarbon families, including persistent ones, such as PAHs. • Bioremediation to remove PAHs from buried and surface oil evaluated through mathematical models. • PAH physic-chemical properties, initial amount and oil contaminated layer depth influence biodegradation. • Degradation was more rapid for PAHs with low concentration and with intermediate hydrophobicity. • Biostimulation and bioaugmentation using autochthonous microorganisms increased removal. • Linear models are suitable tools to assess additive factors/variables influencing bioremediation. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
9. Response of a salt marsh microbial community to antibiotic contamination.
- Author
-
Fernandes, Joana P., Almeida, C. Marisa R., Basto, M. Clara P., and Mucha, Ana P.
- Subjects
- *
SALT marsh plants , *BIOTIC communities , *ANTIBIOTICS , *MICROORGANISMS , *ESTUARINE ecology , *BIOREMEDIATION - Abstract
Salt marsh plants and associated microorganisms can have an important role in contaminant removal from estuaries, through bioremediation processes. Nevertheless, the interaction between emerging contaminants, namely antibiotics, and plant–microorganism associations in estuarine environment are still scarcely known. In this vein, the aim of the present study was to evaluate, in controlled conditions, the response of a salt marsh plant–microorganism association to a contamination with a veterinary antibiotic. For that a salt marsh plant ( Phragmites australis ) and its respective rhizosediment were collected in a temperate estuary (Lima estuary, NW Portugal) and exposed for 7 days to enrofloxacin (ENR) under different nutritional conditions in sediment elutriates. Response was evaluated in terms of ENR removal and changes in microbial community structure (evaluated by ARISA) and abundance (estimated by DAPI). In general, no significant changes were observed in microbial abundance. Changes in bacterial richness and diversity were observed but only in unplanted systems. However, multivariate analysis of ARISA profiles showed significant effect of both the presence of plant and type of treatment on the microbial community structure, with significant differences among all treatment groups. In addition, plants and associated microorganisms presented a potential for antibiotic removal that, although highly dependent on their nutritional status, can be a valuable asset to recover impacted areas such as estuarine ones. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
10. Potential of bioremediation for buried oil removal in beaches after an oil spill.
- Author
-
Pontes, Joana, Mucha, Ana P., Santos, Hugo, Reis, Izabela, Bordalo, Adriano, Basto, M. Clara, Bernabeu, Ana, and Almeida, C. Marisa R.
- Subjects
BIOREMEDIATION ,OIL removal (Sewage purification) ,MARINE ecology ,BEACHES ,OIL spills ,BIODEGRADATION - Abstract
Highlights: [•] Enhancing buried oil remediation is in need. [•] Tests carried out in a specifically designed system with tidal simulation. [•] Biostimulation and autochthonous bioaugmentation (ABA) are suitable options. [•] Microorganisms and nutrients added at the sand column top reached buried oil layer. [•] ABA with suitable nutritive conditions resulted in a faster oil-biodegradation. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
11. Diversity and Hydrocarbon-Degrading Potential of Deep-Sea Microbial Community from the Mid-Atlantic Ridge, South of the Azores (North Atlantic Ocean).
- Author
-
Tomasino, Maria Paola, Aparício, Mariana, Ribeiro, Inês, Santos, Filipa, Caetano, Miguel, Almeida, C. Marisa R., de Fátima Carvalho, Maria, and Mucha, Ana P.
- Subjects
MICROBIAL communities ,MARINE sediments ,SODIUM acetate ,MICROBIAL diversity ,OIL spills ,OCEAN - Abstract
Deep-sea sediments (DSS) are one of the largest biotopes on Earth and host a surprisingly diverse microbial community. The harsh conditions of this cold environment lower the rate of natural attenuation, allowing the petroleum pollutants to persist for a long time in deep marine sediments raising problematic environmental concerns. The present work aims to contribute to the study of DSS microbial resources as biotechnological tools for bioremediation of petroleum hydrocarbon polluted environments. Four deep-sea sediment samples were collected in the Mid-Atlantic Ridge, south of the Azores (North Atlantic Ocean). Their autochthonous microbial diversity was investigated by 16S rRNA metabarcoding analysis. In addition, a total of 26 deep-sea bacteria strains with the ability to utilize crude oil as their sole carbon and energy source were isolated from the DSS samples. Eight of them were selected for a novel hydrocarbonoclastic-bacterial consortium and their potential to degrade petroleum hydrocarbons was tested in a bioremediation experiment. Bioaugmentation treatments (with inoculum pre-grown either in sodium acetate or petroleum) showed an increase in degradation of the hydrocarbons comparatively to natural attenuation. Our results provide new insights into deep-ocean oil spill bioremediation by applying DSS hydrocarbon-degrading consortium in lab-scale microcosm to simulate an oil spill in natural seawater. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
12. Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria.
- Author
-
Perdigão, Rafaela, Almeida, C. Marisa R., Magalhães, Catarina, Ramos, Sandra, Carolas, Ana L., Ferreira, Bruno S., Carvalho, Maria F., and Mucha, Ana P.
- Subjects
BIOREMEDIATION ,MARINE bacteria ,SEAWATER ,RHODOCOCCUS erythropolis ,HYDROCARBONS ,OIL spills ,PETROLEUM - Abstract
This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of Rhodococcus erythropolis and one Pseudomonas sp.), isolated from the NW Portuguese coast, were selected for lyophilization after biomass growth optimization (tested with alternative carbon sources). Results indicated that the bacterial strains remained viable after the lyophilization process, without losing their biodegradation potential. The biomass/petroleum ratio was optimized, and the bioremediation efficiency of the lyophilized bacterial consortium was tested in microcosms with natural seawater and petroleum. An acceleration of the natural oil degradation process was observed, with an increased abundance of oil-degraders after 24 h, an emulsion of the oil/water layer after 7 days, and an increased removal of total petroleum hydrocarbons (47%) after 15 days. This study provides an insight into the formulation and optimization of lyophilized bacterial agents for application in autochthonous oil bioremediation. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
13. Pharmaceutical Compounds in Aquatic Environments—Occurrence, Fate and Bioremediation Prospective.
- Author
-
Fernandes, Joana P., Almeida, C. Marisa R., Salgado, Maria A., Carvalho, Maria F., and Mucha, Ana P.
- Subjects
BIOREMEDIATION ,SEWAGE disposal plants ,NATURAL selection ,IN situ bioremediation ,POLLUTANTS - Abstract
Various contaminants of emerging concern (CECs) have been detected in different ecosystems, posing a threat to living organisms and the environment. Pharmaceuticals are among the many CECs that enter the environment through different pathways, with wastewater treatment plants being the main input of these pollutants. Several technologies for the removal of these pollutants have been developed through the years, but there is still a lack of sustainable technologies suitable for being applied in natural environments. In this regard, solutions based on natural biological processes are attractive for the recovery of contaminated environments. Bioremediation is one of these natural-based solutions and takes advantage of the capacity of microorganisms to degrade different organic pollutants. Degradation of pollutants by native microorganisms is already known to be an important detoxification mechanism that is involved in natural attenuation processes that occur in the environment. Thus, bioremediation technologies based on the selection of natural degrading bacteria seem to be a promising clean-up technology suitable for application in natural environments. In this review, an overview of the occurrence and fate of pharmaceuticals is carried out, in which bioremediation tools are explored for the removal of these pollutants from impacted environments. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
14. Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons.
- Author
-
Perdigão, Rafaela, Almeida, C. Marisa R., Santos, Filipa, Carvalho, Maria F., and Mucha, Ana P.
- Subjects
BIOREMEDIATION ,PETROLEUM ,CONSORTIA ,OIL spills ,SEDIMENTS ,ALIPHATIC hydrocarbons ,HYDROCARBONS - Abstract
Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera Pseudomonas, Rhodococcus and Acinetobacter. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA/P) and bioaugmentation with inoculum pre-grown in acetate (BA/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA/P and BA/A but not in NA. Acetate was the best substrate for consortium growth. BA/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
15. Potential of bacterial consortia obtained from different environments for bioremediation of paroxetine and bezafibrate.
- Author
-
Fernandes, Joana P., Duarte, Patrícia, Almeida, C. Marisa R., Carvalho, Maria F., and Mucha, Ana P.
- Subjects
CONSORTIA ,ESTUARINE sediments ,SEWAGE disposal plants ,BIOREMEDIATION ,DOSAGE forms of drugs - Abstract
• Biodegradation of paroxetine and bezafibrate by bacterial consortia was attempted. • Three of the five consortia displayed removal efficiencies higher than 97 %. • Consortia derived from estuarine sediment displayed better removal efficiencies. • Pseudomonas sp. was dominant in all consortia. • Acinetobacter sp. was recovered in all consortia in static conditions. This work aimed to isolate and identify the culturable bacterial strains from five enriched cultures and investigate the capability of these strains to biodegrade paroxetine (Prx) or bezafibrate (Bzf), when assembled into consortia. Bacterial strains were isolated from cultures enriched with Prx and Bzf using, as microbial inocula, an estuarine sediment and activated sludge from an associated wastewater treatment plant. All the isolated strains were identified through 16S rRNA gene sequencing. Pharmaceutical compounds were analyzed by HPLC-DAD (diode array detector). Fluoride release was analyzed using a fluoride ion-selective electrode to evaluate the extent of paroxetine defluorination. High removal efficiency of pharmaceuticals (>97 %) was observed for three of the five assembled consortia. A total of 28 bacterial strains were isolated from an activated sludge enrichment and 20 bacterial strains were isolated from an estuarine sediment enrichment. The genera Pseudomonas and Acinetobacter were the most dominant in the consortia derived from activated sludge, while the genus Pseudomonas was dominant in the consortia derived from estuarine sediment. This work highlights the potential of native bacterial consortia obtained from different environmental sources to biodegrade Prx or Bzf, and unveils the taxonomic diversity associated to the biodegradation of these compounds. These consortia may be considered in the future for the development of new bioremediation tools for environmental restoration. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
16. Potential of phytoremediation for the removal of petroleum hydrocarbons in contaminated salt marsh sediments.
- Author
-
Ribeiro, Hugo, Mucha, Ana P., Almeida, C. Marisa R., and Bordalo, Adriano A.
- Subjects
- *
PHYTOREMEDIATION , *BIODEGRADATION of hydrocarbons , *HYDROCARBONS & the environment , *BIOREMEDIATION , *JUNCUS , *PHRAGMITES australis - Abstract
Abstract: Degradation of petroleum hydrocarbons in colonized and un-colonized sediments by salt marsh plants Juncus maritimus and Phragmites australis collected in a temperate estuary was investigated during a 5-month greenhouse experiment. The efficiency of two bioremediation treatments namely biostimulation (BS) by the addition of nutrients, and bioaugmentation (BA) by addition of indigenous microorganisms was tested in comparison with hydrocarbon natural attenuation in un-colonized and with rhizoremediation in colonized sediments. Hydrocarbon degrading microorganisms and root biomass were assessed as well as hydrocarbon degradation levels. During the study, hydrocarbon degradation in un-colonized sediments was negligible regardless of treatments. Rhizoremediation proved to be an effective strategy for hydrocarbon removal, yielding high rates in most experiments. However, BS treatments showed a negative effect on the J. maritimus potential for hydrocarbon degradation by decreasing the root system development that lead to lower degradation rates. Although both plants and their associated microorganisms presented a potential for rhizoremediation of petroleum hydrocarbons in contaminated salt marsh sediments, results highlighted that nutrient requirements may be distinct among plant species, which should be accounted for when designing cleanup strategies. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.