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The development of carbon nanotube (CNT)‐based sensors remains an active area of research. Towards this end, a new method for manipulating CNTs, assembling CNT networks and fabricating CNT‐based nanosensors was demonstrated in this study. CNTs were collected and concentrated by optically‐induced dielectrophoresis (ODEP) forces and aligned between a pair of electrodes. This assembly was then used directly as a temperature sensor and a hot‐film anemometer, which detects changes in windspeed. By offering efficient CNT collection and ready‐to‐use sensor fabrication, this ODEP‐based approach presents a promising method for the development of CNT‐based sensing applications and massively parallel assembly of CNT‐lines. The developed CNT‐based nanosensors may be used to measure the temperature and the flow velocity of bio‐samples in the near future. [ABSTRACT FROM AUTHOR]