1. Electrochemiluminescent biosensor with DNA link for selective detection of human IgG based on steric hindrance.
- Author
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Liu Q, Xie XL, Mao CJ, Chen JS, Niu HL, and Song JM
- Subjects
- Cadmium Compounds chemistry, Electrochemistry, Electrodes, Europium chemistry, Gold chemistry, Humans, Immunoglobulin G chemistry, Models, Molecular, Nucleic Acid Conformation, Nucleic Acid Hybridization, Quantum Dots chemistry, Selenium Compounds chemistry, Biosensing Techniques methods, DNA chemistry, Immunoglobulin G analysis, Limit of Detection, Luminescent Measurements
- Abstract
A highly selective DNA-based electrochemiluminescence (ECL) based biosensor is described for the detection of human IgG. It is exploiting the effect of steric hindrance that affects the strength of the ECL signal in the presence of IgG. Digoxin-linked signaling DNA was specifically bound to IgG, and this causes steric hindrance which limits the ability of DNA to hybridize with capturing DNA attached to a gold electrode. Europium (II) doped CdSe quantum dots were covalently linked to the DNA in order to generate the ECL signal. Using this steric hindrance hybridization method, the ECL signal of the biosensor were proportional to the concentration of IgG with a wide linear range and a 14 pM detection limit. Conceivably, the method can be expanded to the detection of a wide range of proteins for which homologous recognition elements are available., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
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