1. Impedimetric aptasensor for tobramycin detection in human serum
- Author
-
González-Fernández, Eva, de-los-Santos-Álvarez, Noemí, Lobo-Castañón, María Jesús, Miranda-Ordieres, Arturo José, and Tuñón-Blanco, Paulino
- Subjects
- *
ELECTROCHEMISTRY , *TOBRAMYCIN , *SERUM , *RNA , *IMPEDANCE spectroscopy , *BIOSENSORS , *ANTIBIOTICS , *SOLUTION (Chemistry) , *AMINOGLYCOSIDES - Abstract
Abstract: An RNA aptamer is proposed as a recognition element for the detection of tobramycin in human serum. A displacement assay was developed using faradaic-electrochemical impedance spectroscopy (F-EIS) as a detection technique. Two modified aptamers, a partially (ATA) and a fully O-methylated aptamer (FATA) were evaluated and compared. The affinity constant, K D, for both aptamers was estimated by F-EIS resulting virtually identical within the experimental error. The selectivity towards other aminoglycosides was also studied. The analytical characteristics were evaluated in aqueous solution using both aptamers and FATA was selected for human serum experiments. Using a 1:0.5 dilution of the serum, a linear range between 3μM and 72.1μM was obtained, which included the therapeutic range of the antibiotic. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF