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We have developed a novel nitric oxide (NO) cellular biosensor based upon the immobilization of red blood cells (RBCs) onto nanometer‐size colloidal gold that is attached to an electrochemically pretreated glassy carbon electrode via the bridging of an ethylenediamine monolayer. The biosensor has been characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and electrochemistry. The immobilized RBCs display an excellent electrocatalytic response to nitric oxide. The electrocatalytic currents are proportional to the NO concentration in the range from 1.0×10–8 to 1.0×10–6 M and the detection limit is as low as 5.0×10–9 M (S/N=3). Furthermore, the biosensor is very stable and relatively free of potential interference. [ABSTRACT FROM AUTHOR]

A dual-functional electrochemical biosensor for adenosines and hydrogen peroxide from cancer cells was developed based on a traditional switchable electrochemical sensing format and ATP improved G-quadruplex DNAzyme as a biolabel. [ABSTRACT FROM AUTHOR]

Nanochannels have brought new opportunities for biosensor development. Herein, we present the novel concept of a nanochannels photoelectrochemical (PEC) biosensor based on the integration of a unique CuxO-nanopyramid-islands (NPIs) photocathode, an anodic aluminum oxide (AAO) membrane, and alkaline phosphatase (ALP) catalytic chemistry. The CuxO-NPIs photocathode possesses good performance, and further assembly with AAO yields a designed architecture composed of vertically aligned, highly ordered nanoarrays on top of the CuxO-NPIs film. After biocatalytic precipitation (BCP) was stimulated within the channels, the biosensor was used for the successful detection of ALP activity. This study has not only provided a novel paradigm for an unconventional nanochannels PEC biosensor, which can be used for general bioanalytical purposes, but also indicated that the new concept of nanochannel -- semiconductor heterostructures is a step toward innovative biomedical applications. [ABSTRACT FROM AUTHOR]