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1. In situ labelling chemistry of respiratory syncytial viruses by employing the biotinylated host-cell membrane protein for tracking the early stage of virus entry.

Author

Zheng LL, Yang XX, Liu Y, Wan XY, Wu WB, Wang TT, Wang Q, Zhen SJ, and Huang CZ

Subjects
Biotin metabolism, Biotinylation, Cell Line, Fluorescent Dyes chemistry, Humans, Membrane Proteins metabolism, Microscopy, Confocal, RNA, Viral chemistry, RNA, Viral metabolism, Streptavidin metabolism, Temperature, Time-Lapse Imaging, Virus Internalization, Biotin chemistry, Membrane Proteins chemistry, Quantum Dots chemistry, Respiratory Syncytial Viruses physiology, Streptavidin chemistry
Abstract

An in situ labelling strategy was proposed to produce quantum dot-labelled respiratory syncytial viruses (RSVs) by incorporating the biotinylated membrane protein of the host cells into mature virions, followed by conjugation with streptavidin modified quantum dots (SA-QDs), which has the advantages such as convenience, efficiency and minor influence on viral infectivity and thus could be successfully applied to track the early stage of virus entry.

Published
2014
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