1. Anticancer effect of X-Ray triggered methotrexate conjugated albumin coated bismuth sulfide nanoparticles on SW480 colon cancer cell line.
- Author
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Faghfoori MH, Nosrati H, Rezaeejam H, Charmi J, Kaboli S, Johari B, and Danafar H
- Subjects
- Antimetabolites, Antineoplastic chemistry, Apoptosis drug effects, Apoptosis radiation effects, Bismuth chemistry, Cell Line, Tumor, Cell Survival drug effects, Cell Survival radiation effects, Colonic Neoplasms pathology, Drug Compounding, Humans, Methotrexate chemistry, Radiation-Sensitizing Agents chemistry, Serum Albumin, Bovine chemistry, Sulfides chemistry, Antimetabolites, Antineoplastic pharmacology, Bismuth pharmacology, Chemoradiotherapy, Colonic Neoplasms therapy, Drug Carriers, Methotrexate pharmacology, Nanoparticles, Radiation-Sensitizing Agents pharmacology, Serum Albumin, Bovine pharmacology, Sulfides pharmacology
- Abstract
The application of nanoparticles (NPs) as radio-sensitizers and carriers has opened up a new horizon to overcome the limitations of chemo and radiotherapy. In this study, bovine serum albumin-coated Bi
2 S3 NPs (Bi2 S3 @BSA NPs) were synthesized and evaluated in terms of their ability to be used as a radio-sensitizer and carrier for methotrexate (MTX). Physicochemical properties of MTX conjugated Bi2 S3 @BSA NPs (Bi2 S3 @BSA-MTX NPs) were characterized by DLS, TEM, FTIR, UV/Vis, and XRD analyses. After the evaluation of cellular uptake and intracellular localization, the cytotoxicity of the combination of Bi2 S3 @BSA-MTX NPs and X-Ray radiation was analyzed against the SW480 cell line. The synthesized NPs exhibited spherical-like shapes and homogenous morphology, possessing a hydrodynamic diameter of 140.2 ± 5.71 nm (mean ± SD) and zeta potential of -25 mV. Also, the release study showed that the release of MTX is faster and higher in the presence of the proteinase K enzyme than the absence of the enzyme. The results of in-vitro chemo-radiation therapy indicated that the viability of treated cells with Bi2 S3 @BSA-MTX NPs is significantly lower than the cells treated with Bi2 S3 @BSA NPs. Furthermore, cells treated with Bi2 S3 @BSA-MTX NPs showed a lower degree of viability when combined with X-Ray radiation in comparison with the absence of irradiation, which confirmed the ability of the Bi2 S3 @BSA-MTX NPs as radio-sensitizer., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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