1. Comparison of laser-assisted hatching and acidified Tyrode's hatching by evaluation of blastocyst development rates in sibling embryos: a prospective randomized trial.
- Author
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Jones AE, Wright G, Kort HI, Straub RJ, and Nagy ZP
- Subjects
- Adult, Blastocyst cytology, Blastocyst drug effects, Blastocyst radiation effects, Embryo Culture Techniques, Embryonic Development drug effects, Embryonic Development radiation effects, Female, Fertilization in Vitro, Humans, Hydrogen-Ion Concentration, Isotonic Solutions chemistry, Pregnancy, Pregnancy Rate, Preimplantation Diagnosis, Prospective Studies, Blastocyst physiology, Isotonic Solutions therapeutic use, Lasers, Reproductive Techniques, Assisted, Zona Pellucida drug effects, Zona Pellucida radiation effects
- Abstract
Objective: To assess two zona drilling methods in terms of blastocyst development rates using sister embryos., Design: Prospective, randomized study. Sister embryos of 14 patients were randomly assigned on day 3 to acidified Tyrode's zona drilling or to laser zona drilling. After biopsy, subsequent embryo culture until the blastocyst stage (day 5) was performed., Setting: Private fertility center., Patient(s): Patients undergoing IVF-preimplantation genetic diagnosis., Intervention(s): Embryo biopsy using either laser-assisted hatching or acidified Tyrode's hatching on sibling embryos and subsequent blastocyst development evaluation., Main Outcome Measure(s): Evaluation of blastocyst development in terms of degree of expansion and cell number in the inner cell mass and trophectoderm., Result(s): Blastocyst development rates (and blastocyst quality) were similarly high in both the acidified Tyrode's hatching group and the laser-assisted hatching group., Conclusion(s): Laser hatching does not impair embryonic development to the blastocyst stage, demonstrating that laser-assisted hatching is a suitable alternative to the use of acidified Tyrode's solution for zona drilling.
- Published
- 2006
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