1. Microchimerism as Post-Transplant Marker of a Chronic Rejection Process.
- Author
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Sieńko, Jerzy, Kotowski, Maciej, Czarnecka, Wiktoria, Podkówka, Albert, Tejchman, Karol, Kotfis, Katarzyna, Zeair, Samir, Czajkowski, Zenon, and Skonieczna-Żydecka, Karolina
- Subjects
CYSTATIN C ,KIDNEY transplantation ,BLOOD sampling ,UREA ,URINE - Abstract
The risk of losing a transplanted organ is high, and non-invasive markers to warn of this phenomenon are still being sought. We investigated the impact of post-transplant microchimerism on the function of the transplanted kidney. The study included 100 kidney transplant recipients, mostly women. All transplanted organs were from opposite-sex deceased donors. Microchimerism was assessed using multiplex PCR. Male DNA was detected in all urine samples from female recipients and in 13/56 blood samples from female kidney recipients. Female DNA was found in 31/44 urine samples from male recipients, but in none of the blood samples. Microchimerism in the urine of female recipients correlated positively with blood urea (Rs = 0.45; p = 5.84 × 10
−4 ) and K+ ions (Rs = 0.29; p = 0.03), while microchimerism in the blood of female recipients also correlated positively with blood urea (Rs = 0. 28; p = 0.04), cystatin C (Rs = 0.31; p = 0.02) and the number of incompatible HLA alleles (Rs = 0.42; p = 0.01). A history of DGF was associated with higher urinary donor DNA concentrations in female recipients.: Post-transplant microchimerism may serve as a potential marker of chronic kidney rejection. [ABSTRACT FROM AUTHOR]- Published
- 2023
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