1. Nearly half of Ultrio plus NAT non-discriminated reactive blood donors were identified as occult HBV infection in South China.
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Ye, Xianlin, Li, Tong, Shao, Wen, Zeng, Jinfeng, Hong, Wenxu, Lu, Liang, Zhu, Weigang, Li, Chengyao, and Li, Tingting
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BLOOD donors , *BLOOD plasma , *INFECTION , *OCCULTISM , *BLOOD sampling , *HEPATITIS B , *HEPATITIS viruses , *HIV , *GENETIC mutation , *POLYMERASE chain reaction , *SERODIAGNOSIS , *VIRAL antibodies , *VIRAL antigens , *GENOTYPES - Abstract
Background: Blood donor plasma samples were detected by the Ultrio Plus NAT system for HBV, HCV and HIV-1 in Shenzhen blood center, China. Reactive samples underwent further discriminatory testing of a single virus by the same methodology. A large number of cases of non-discriminated reactive (NDR) donors were found, leaving potential risk of transmitting HBV if not deferrals. This study identified those non-discriminated samples.Methods: The NDR plasma samples from blood donation screening were detected and classified by additional molecular and serological tests. Molecular characterizations of DNA+ NDR were determined by sequencing analysis.Results: A number of 259 (0.21%) NDR plasma samples from screening of 123,280 eligible blood donors were detected, which presented a higher rate (91.1%) of anti-HBc reactivity and nearly half (46.7%) of HBV DNA+ that classified as occult HBV infection (OBI). Most OBI strains were wild-type HBV, but some substitutions V168A, S174 N, V177A, Q129R/L/H, G145A/R in S region of genotype B (OBIB) and T47K/V/A, P49H/L, Q101R/H/K, S174 N, L175S, V177A, T118 M/R/K, G145R/A/K/E, R160K/N in S region of genotype C (OBIC) strains were identified in high frequency.Conclusion: Nearly half of NDR blood samples were identified as OBI, in which a number of important mutations were detected. NDR donation might have potential risk for HBV transmission, but need to be further investigated. [ABSTRACT FROM AUTHOR]- Published
- 2019
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