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Introduction: Type 2 diabetes mellitus is an important metabolic disease that causes disability and involves small and large vessels. Many herbal drugs have been used for the treatment of high blood sugar (BS) with promising results. One of these medicinal drugs used conventionally in medicine by the people and active physicians is Otostegia persica. The aim of this study was to investigate the effect of O. persica extract on blood glucose in diabetic patients with insulin therapy. Materials and Methods: A total of 56 patients with type 2 diabetes were included in the study and were divided into two groups of 28 each. The intervention and nonintervention groups included 22 (78.6%) and 6 (21.4%) female patients as well as 19 (67.9%) and 9 (32.1%) male patients, respectively. Results: Due to homogenization resulting from random selection among aspartate transaminase, alanine transaminase, creatinine, insulin, blood urea nitrogen, Antibiotics (Antib), An mdaB mutant strain in a quinone reductase (MdaB) variables, significant differences were not observed between the two groups of participants before the study. Furthermore, there was no significant difference among the variables of H1c, BS5, HD1, fasting BS (FBS), low-density lipoprotein (LDL), and triglycerides in two groups before the study. However, there were differences among variables H1c, BS5, FBS, high-density lipoprotein (HDL), LDL, and HDL between the two groups of participants after the study, but they were not statistically significant (P > 0.05). Conclusions: The results of the test did not show statistically significant differences among these variables during 3 months. Therefore, in order to conclude more comprehensive and safer application for diabetes treatment, it is suggested to examine the effects of these drugs in a more extensive area using oral and injection doses as well as investigate pure effects of active materials. [ABSTRACT FROM AUTHOR]