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The present study investigated whether increasing morning rectal temperatures (T rec ) to resting.evening levels, or decreasing evening T rec or muscle (T m ) temperatures to morning values, would influence repeated sprint (RS) performance in a causal manner. Twelve trained males underwent five sessions [age (mean ± SD) 21.8 ± 2.6 yr, peak oxygen uptake ([Formula: see text] peak) 60.6 ± 4.6 mL kg min -1 , stature 1.78 ± 0.07 m and body mass 76.0 ± 6.3 kg]. These included a control morning (M, 07:30 h) and evening (E, 17:30 h) session (5-min warm-up), and three further sessions consisting of a warm-up morning trial (M E , on a motorised treadmill) until T rec reached evening levels; and two cool-down evening trials (in 16-17°C water) until T rec (E Mrec ) or T m (E Mmuscle ) values reached morning temperatures, respectively. All sessions included a 3 × 3-s task-specific warm-up followed by 10 × 3-s RS with 30-s recoveries performed on a non-motorised treadmill. T rec and T m measurements were taken at the start of the protocol and following the warm-up or cool-down period. Values for T rec and T m were higher in the evening compared to morning values (0.45°C and 0.57°C, P < 0.05). RS performance was lower in the M for distance covered (DC), average power (AP) and average velocity (AV) (9-10%, P < 0.05). Pre-cooling T rec and T m in the evening reduced RS performance to levels observed in the morning (P < 0.05). However, an active warm-up resulted in no changes in morning RS performance. Diurnal variation in T rec and T m is not wholly accountable for time-of-day oscillations in RS performance on a non-motorised treadmill; the exact mechanism(s) for a causal link between central temperature and human performance are still unclear and require more research.

In active males, muscle force production and short-term (<6 s) anaerobic performance are significantly greater in the evening compared with the morning. This diurnal variation is attributed to motivational, peripheral and central factors, and possibly the higher core and muscle temperatures observed in the evening. However, little is known regarding whether diurnal variation on a treadmill also exists in team-sport specific tests of repeated sprint ability (RSA), as would be relevant to football, for example. A controlled laboratory protocol using a non-motorised treadmill has been used to investigate whether daily variation in RSA is present in highly motivated athletes. Twenty active males (mean ± SD: age, 21.0 ± 2.2 yrs; maximal oxygen uptake ([Formula: see text] max), 60.8 ± 4.8 ml kg min(-1); body mass, 77.02 ± 10.5 kg and height, 1.79 ± 0.07 m) volunteered and completed two sessions counterbalanced in order of administration (separated by >48 h): a morning (M, 07:30 h) and evening (E, 17:30 h) session. Both sessions included a 5-min active warm-up on a motorised treadmill at 10 km h(-1) followed by three task-specific warm-up sprints at 50%, 70% and 80%, respectively, on a non-motorised treadmill. During each trial, 10 × 3 s repeated sprints with 30 s recoveries were performed on the non-motorised treadmill. Rectal (Trec) and muscle temperature measurements (Tm) were taken after subjects had reclined for 30 min at the start of the protocol, and again after the active warm-up. Values of heart rate, thermal comfort (TC), rating of perceived exertion (RPE) and effort were measured throughout. Blood samples were taken at rest, after the sprints and 5-min post sprints. Data were analysed using a GLM with repeated measures. Trec and Tm values were higher at rest in the evening than the morning (0.46 °C and 0.57 °C, respectively, p < 0.05). Distance covered, peak power, average power, peak velocity and average velocity all showed significantly higher values in the evening compared with the morning (a range of 3.3-8.3%, p < 0.05), with peak power displaying a statistical trend (0.10 > p > 0.05). All subjects reported maximal values for "effort" for each sprint. There were significant positive correlations between Trec and Tm, Trec and RPE, TC and all measures of RSA performance. However, there was no correlation between fatigue index for peak power output or peak velocity and Trec. In summary, in this population of motivated subjects, time-of-day effects were seen in resting Trec and Tm values and all performance measures of RSA, in partial agreement with past research. The diurnal variation in Trec and Tm cannot fully explain time-of-day oscillations in RSA on a non-motorised treadmill. Although central temperature may provide some endogenous rhythm to human performance, the causal link seems to be due to a multiplicity of components and mechanisms.

Muscle force production and power output in active males, regardless of the site of measurement (hand, leg, or back), are higher in the evening than in the morning. This diurnal variation is attributed to motivational, peripheral and central factors, and higher core and, possibly, muscle temperatures in the evening. This study investigated whether increasing morning rectal temperatures to evening resting values, by active or passive warm-ups, leads to muscle force production and power output becoming equal to evening values in motivated subjects. Ten healthy active males (mean ± SD: age, 21.2 ± 1.9 yrs; body mass, 75.4 ± 8 kg; height, 1.76 ± .06 m) completed the study, which was approved by the University Ethics Committee. The subjects were familiarized with the techniques and protocol and then completed four sessions (separated by at least 48 h): control morning (07:30 h) and evening (17:30 h) sessions (with an active 5-min warm-up) and then two further sessions at 07:30 h but proceeded by an extended active or passive warm-up to raise rectal temperature to evening values. These last two sessions were counterbalanced in order of administration. During each trial, three measures of handgrip strength, isokinetic leg strength measurements (of knee flexion and extension at 1.05 and 4.19 rad.s(-1) through a 90° range of motion), and four measures of maximal voluntary contraction (MVC) on an isometric ergometer (utilizing the twitch-interpolation technique) were performed. Rectal and intra-aural temperatures, ratings of perceived exertion (RPE) and thermal comfort (TC) were measured. Measurements were made after the subjects had reclined for 30 min and after the warm-ups and prior to the measurement of handgrip and isokinetic and isometric ergometry. Muscle temperature was taken after the warm-up and immediately before the isokinetic and MVC measurements. Warm-ups were either active (cycle ergometer at 150 W) or passive (resting in a room at 35 °C, relative humidity 45%). Data were analyzed using analysis of variance models with repeated measures. Rectal and intra-aural temperatures were higher at rest in the evening (.56 °C and .74 °C; p < .05) than in the morning, but there were no differences after the active or passive warm-ups, the subjects' ratings of thermal comfort reflecting this. Muscle temperatures also displayed significant diurnal variation, with higher values in the evening (~.31 °C; p < .05). Grip strength, isokinetic knee flexion for peak torque and peak power at 1.05 rad.s(-1), and knee extension for peak torque at 4.19 rad.s(-1) all showed higher values in the evening. All other measures of strength or power showed a trend to be higher in the evening ( .10 > p > .05). There was no significant effect of active or passive warm-ups on any strength or power variable, and subjects reported maximal values for effort for each strength measure. In summary, effects of time of day were seen in some measures of muscle performance but, in this population of motivated subjects, there was no evidence that increasing morning rectal temperature to evening values by active or passive warm-up increased muscle strength to evening values.

Few functional measures related to time-trial display diurnal variation. The diversity of tests/protocols used to assess time-trial performance on diurnal effects and the lack of a standardised approach hinder agreement in the literature. Therefore, the aims of the present study were to investigate and systematically review the evidence relating to time-of-day differences in time-trial measures and to examine the main aspects related to research design important for studies of a chronobiological nature. The entire content of Manipal Academy of Higher Education electronic library and Qatar National Library, and electronic databases: PubMed (MEDLINE), Scopus and Web of Science were searched. Research studies published in peer-reviewed journals and non-peer reviewed studies, conducted in male adult participants aged ≥18 y before November 2021 were screened/included. Studies assessing tests related to time-trials in any modality between a minimum of 2 time-points during the day (morning [06:30–10:30 h] vs evening [14:30–20:00 h]) were deemed eligible. The Grading of Recommendations Assessment, Development, and Evaluation (GRADE) process was used to assess the evidence to inform recommendations. The primary search revealed that a total of 10 from 40 articles were considered eligible and subsequently included. Six were conducted using cycling, two using running and two using swimming as the mode of exercise. Distances ranged from 1 to 16.1-km in distance or 15 to 20-min time in the cycling and running time-trials, and 50 to 200-m in the swimming time-trials. Only four studies found one or several of their performance variables to display daily variations, with significantly better values in the evening; while six studies found no time-of-day significance in any variables assessed. The magnitude of difference ranged from 2.9% to 7.1% for performance time to complete a cycling time-trial, while running and swimming did not find any differences for performance time. Power output during a 16.1-km time trial in cycling also found evening performance to be significantly better by 10%. The only other observed differences were stroke rate and stroke length during a swimming time-trial and stroke rate (cycles.min−1) during a cycling time-trial. The magnitude of difference is dependent on exercise modality, individual chronotype, the training status of the individual and sample size differences. The lack of diurnal variation present in the majority of studies can in-part be explained with some of the methodological limitations and issues present related to quality and control. It is paramount that research assessing diurnal variation in performance uses appropriate session timings around the core body temperature minimum (~05:00 h; morning) and maximum (~17:00 h; evening). Although, differences in motivation/arousal, habitual training times, chronotypes and genotypes could provide an explanation as to why some studies/variables did not display time-of-day variation, more work is needed to provide an accurate conclusion. There is a clear demand for a rigorous, standardised approach to be adopted by future investigations which control factors that specifically relate to investigations of time-of-day, such as appropriate familiarisation, counterbalancing the order of administration of tests, providing sufficient recovery time between sessions and testing within a controlled environment. [ABSTRACT FROM AUTHOR]

Muscle force production and power output in active males, regardless of the site of measurement (hand, leg, or back), are higher in the evening than the morning. This diurnal variation is attributed to motivational, peripheral, and central factors and higher core and, possibly, muscle temperatures in the evening. This study investigated whether decreasing evening resting rectal temperatures to morning values, by immersion in a water tank, leads to muscle force production and power output becoming equal to morning values in motivated subjects. Ten healthy active males (mean ± SD: age, 22.5 ± 1.3 yrs; body mass, 80.1 ± 7.8 kg; height, 1.72 ± 0.05 m) completed the study, which was approved by the local ethics committee of the university. The subjects were familiarized with the techniques and protocol and then completed three sessions (separated by at least 48 h): control morning (07:30 h) and evening (17:30 h) sessions (with an active 5-min warm-up on a cycle ergometer at 150 W) and then a further session at 17:30 h but preceded by an immersion in cold water (∼16.5 °C) to lower rectal temperature (Trec) to morning values. During each trial, three measures of grip strength, isokinetic leg strength measurements (of knee flexion and extension at 1.05 and 4.19 rad s−1 through a 90° range of motion), and three measures of maximal voluntary contraction (MVC) on an isometric dynamometer (utilizing the twitch-interpolation technique) were performed. Trec, rating of perceived exertion (RPE), and thermal comfort (TC) were also measured after the subjects had reclined for 30 min at the start of the protocol and prior to the measures for grip, isokinetic, and isometric dynamometry. Muscle temperature was taken after the warm-up or water immersion and immediately before the isokinetic and MVC measurements. Data were analyzed using general linear models with repeated measures. Trec values were higher at rest in the evening (by 0.37 °C; p < 0.05) than the morning, but values were no different from morning values immediately after the passive pre-cooling. However, Trec progressively decreased throughout the experiments, this being reflected in the subjects' ratings of thermal comfort. Muscle temperatures also displayed significant diurnal variation, with higher values in the evening (by 0.39 °C; p < 0.05). Right grip strength, isometric peak power, isokinetic knee flexion and extension for peak torque and peak power at 1.05 rad s−1, and knee extension for peak torque at 4.19 rad s−1 all showed higher values in the evening (a range of 3-14%), and all other measures of strength or power showed a statistical trend to be higher in the evening (0.10 > p > 0.05). Pre-cooling in the evening significantly reduced force or power variables towards morning values. In summary, effects of time of day were seen in some measures of muscle performance, in agreement with past research. However, in this population of motivated subjects, there was evidence that decreasing evening Trec to morning values by coldwater immersion decreased muscle strength to values similar to those found in the morning. It is concluded that diurnal changes in muscle performance are linked to diurnal changes in Trec. (Author correspondence: B.J.Edwards@ljmu.ac.uk) [ABSTRACT FROM AUTHOR]