1. Diverse niches within multiple myeloma bone marrow aspirates affect plasma cell enumeration.
- Author
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Nadav, Liat, Katz, Ben-Zion, Baron, Shoshana, Yossipov, Lydia, Polliack, Aaron, Deutsch, Varda, Geiger, Benjamin, and Naparstek, Ellizabeth
- Subjects
MULTIPLE myeloma ,PLASMA cells ,BONE marrow ,IMMUNE system ,LYMPHOID tissue - Abstract
A basic criterion for the diagnosis of multiple myeloma is plasma cell enumeration within the bone marrow (BM). This report showed that flow cytometry under-estimated the number of plasma cells in BM aspirates by an average of 60%, compared with morphological evaluation. The discrepancy was partially because BM smears contain cells associated with the lipid-enriched spicules. In contrast, flow cytometry is performed on the BM fluid, which is depleted of the lipid-adhesive plasma cells. This discrepancy may point to different plasma cell subpopulations associated with diverse niches within the BM. [ABSTRACT FROM AUTHOR]
- Published
- 2006
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