1. Adult bone marrow cells differentiate into neural phenotypes and improve functional recovery in rats following traumatic brain injury.
- Author
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Lu J, Moochhala S, Moore XL, Ng KC, Tan MH, Lee LK, He B, Wong MC, and Ling EA
- Subjects
- 2',3'-Cyclic-Nucleotide Phosphodiesterases metabolism, Animals, Brain Injuries pathology, Brain Injuries physiopathology, Cell Differentiation, Cell Movement, Cerebral Cortex metabolism, Cerebral Cortex pathology, Glial Fibrillary Acidic Protein metabolism, Immunohistochemistry, Intermediate Filament Proteins metabolism, Male, Nerve Tissue Proteins metabolism, Nestin, Neurons metabolism, Neurons pathology, Organ Specificity, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Stromal Cells transplantation, Tubulin metabolism, Bone Marrow Cells cytology, Bone Marrow Transplantation, Brain Injuries therapy
- Abstract
This study aims to investigate the therapeutic potential of adult bone marrow stromal cells (BMSCs). Exposed to a cocktail of induction medium, some BMSCs could differentiate into cell types with phenotypes of neural lineages in vitro. These cells expressed neural markers nestin, GFAP, 68-kDa neurofilament and beta-tubulin III as detected by immunohistochemistry and RT-PCR. Fluorescence-labeled cells were injected intravenously at 72 h after traumatic brain injury. Transplanted cells survived and migrated to the ipsilateral cerebral cortex at different time points after injection. They were immunopositive for neuronal marker MAP-2, oligodendrocyte marker CNPase, astrocytic maker GFAP or microglial marker OX-42 in vivo. In rats receiving BMSC transplants, there were significant improvements in motor and neurological functions when compared with the control groups. Hence, the therapeutic potential of BMSCs for traumatic brain injury is further amplified.
- Published
- 2006
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