1. Species variation in the spontaneous calcification of bone marrow-derived mesenchymal stem cells.
- Author
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Huang YZ, Cai JQ, Lv FJ, Xie HL, Yang ZM, Huang YC, and Deng L
- Subjects
- Animals, Bone Marrow Cells metabolism, Goats physiology, Humans, Mesenchymal Stem Cells metabolism, Rats, Species Specificity, Bone Marrow Cells pathology, Bone Regeneration, Calcinosis, Mesenchymal Stem Cells pathology
- Abstract
Bone marrow-derived mesenchymal stem cells (BM-MSCs) hold great promise for tissue regeneration. With increasing numbers of clinical trials, the safety of BM-MSCs attracts great interest. Previously, we determined that rat BM-MSCs possessed spontaneous calcification without osteogenic induction after continuous culture. However, it is unclear whether BM-MSCs from other species share this characteristic. In this study, spontaneous calcification of BM-MSCs from rat, goat, and human specimens was investigated in vitro. BM-MSCs were cultured in complete medium, and calcification was determined by morphologic observation and alizarin red staining. It was demonstrated that rat BM-MSCs possessed a typically spontaneous calcification, whereas goat and human BM-MSCs under the same system proliferated significantly but did not calcify spontaneously. The significant species variation in spontaneous calcification of BM-MSCs described in this study provides useful information regarding evaluation of numerous BM-MSC-based approaches for bone regeneration and the safety of BM-MSCs., (Copyright © 2013 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.)
- Published
- 2013
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