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Simple Summary: Correction of bone volume loss after tooth extractions, periodontal lesions or trauma is performed by augmentation procedures using various biomaterials used to fill bone defects. Tissue engineering searching, often using a combination of stem cells, particles and growth factors, is making significant progress in this field. An alternative is the use of autologous tooth hard tissue grit obtained from extracted teeth. During the demineralization of the grit, the organic components of the tooth tissue, the dentin matrix, which has a confirmed high osteogenic effect, is exposed. This work evaluates the stimulatory effect of some demineralization procedures on the exposure of the dentin matrix and thus on the improvement in their osteogenic properties. The issue of bone volume loss is playing an increasing role in bone tissue engineering. Research has focused on studying the preparation and use of different types of human or xenogenic materials and their osteogenic properties. An alternative source for this purpose could be autologous extracted teeth. The simple preparation protocol, minimal immune response, and rapid organizing of the newly formed bone with optimal mechanical properties predispose autologous hard teeth tissues (HTTs) as a promising material suitable in the indication of augmentation of maxillary and mandible defects, comparable to other high-end augmentation materials. The aim of this study was to experimentally evaluate the osteogenic potential of ground native autologous HTTs prepared by different demineralization procedures, aimed at potentiating the osteoinductive and osteoconductive properties of their organic components. The results indicate that the most effective preparation process for HTT stimulation is the application of Cleanser for 10 min followed by exposure to 0.6 N HCl for 5 min with a wash in phosphate-buffered saline solution. [ABSTRACT FROM AUTHOR]