1. Experimental infection of pregnant pyrenean chamois (rupicapra pyrenaica) with border disease virus subtype 4
- Author
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Martin, Claire, Duquesne, Véronique, Guibert, Jean-Michel, Pulido, Coralie, Gilot-Fromont, Emmanuelle, Gibert, Philippe, Velarde, Roser, Thiéry, Richard, Marco Sánchez, Ignasi, Dubois, Eric, Institut universitaire de formation des maîtres - Midi-Pyrénées (IUFM Midi-Pyrénées), Université Toulouse - Jean Jaurès (UT2J), Laboratoire d'études et de recherches sur les petits ruminants et les abeilles, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Biodémographie évolutive, Département écologie évolutive [LBBE], Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Unit fo Ruminant Pathology, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Servei d'Ecopatologia de Fauna Salvatge (SEFaS), Departament de Medicina i Cirurgia Animals, Edifici V, Facultat de Veterinària, Universitat Autònoma de Barcelona (UAB), CITI, Centre de Recherche Public Henri-Tudor [Luxembourg] (CRP Henri-Tudor), Université de Toulouse (UT)-Université de Toulouse (UT), and Laboratoire d'études et de recherches sur les petits ruminants et les abeilles (LERPRA)
- Subjects
Pyrenean chamois ,MESH: Border disease virus ,[SDV]Life Sciences [q-bio] ,animal diseases ,viruses ,MESH: Border Disease ,MESH: Rupicapra ,Serology ,0403 veterinary science ,Border disease virus ,MESH: Pregnancy ,Rupicapra pyrenaica ,Pregnancy ,MESH: Animals ,Pregnancy Complications, Infectious ,Experimental infection ,0303 health sciences ,Ecology ,biology ,Goats ,virus diseases ,04 agricultural and veterinary sciences ,Abortion, Veterinary ,Rupicapra ,3. Good health ,Remugants ,MESH: RNA, Viral ,RNA, Viral ,Female ,France ,Encephalitis ,medicine.medical_specialty ,040301 veterinary sciences ,Viremia ,Animals, Wild ,MESH: Goat Diseases ,MESH: Goats ,Virus ,MESH: Abortion, Veterinary ,03 medical and health sciences ,MESH: Spain ,medicine ,Animals ,Pathogenicity ,MESH: Pregnancy Complications, Infectious ,MESH: Animals, Wild ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Goat Diseases ,Border Disease ,medicine.disease ,biology.organism_classification ,Virology ,MESH: France ,Spain ,Pestivirus ,Malalties ,Isards ,Histopathology ,Virosi ,MESH: Female ,Animals salvatges - Abstract
Altres ajuts: ANSES/A06-018-2 Border disease virus (BDV) causes high mortality in Pyrenean chamois (Rupicapra pyrenaica) on the French and Spanish sides of the Pyrenees Mountains. We investigated the pathology induced by BDV in pregnant chamois via experimental infection. Three females were inoculated during the second third of pregnancy with a BDV-4 subgroup strain isolated from a wild Pyrenean chamois during an acute epizootic. A fourth pregnant chamois and one nonpregnant ewe were kept as negative controls. Animals were monitored to assess clinical signs, hematology, viremia, and serology. Postmortem examinations included necropsy, histopathology, and quantification of viral RNA in organs. Pregnancy was unsuccessful in all inoculated animals. One died 24 days postinoculation (dpi) without showing any precursory clinical signs. The second animal had profuse diarrhea from 13 dpi to its death at 51 dpi. The third aborted at 46 dpi and was euthanized at 51 dpi. All animals were viremic from 4 dpi until death. Neutralizing antibodies against BDV-4 were detected from 12 dpi. Necropsies showed generalized lymphadenomegaly, associated in one case with disseminated petechial hemorrhages in the digestive tract. Seventy-eight of 79 organs from inoculated adults and their fetuses had detectable viral RNA. The main histologic lesions in adults were mild lymphohistiocytic encephalitis associated with moderate or moderately severe lymphoid depletion. Control animals remained negative for virus (in blood and organs), antibody, and lesions upon postmortem examination. BDV infection during pregnancy in Pyrenean chamois causes severe disease leading to abortion, then death. Despite 100% fetal death following inoculation, viral RNA was recovered from all organs of infected fetuses, suggesting that persistently infected offspring could be born. Our results may help explain the reported decrease in chamois populations in several areas and suggest that great care must be taken when interpreting infection status for wildlife.
- Published
- 2013