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Your search keyword '"Mondardini V."' showing total 6 results
Start Over Author "Mondardini V." Topic borrelia burgdorferi
6 results on '"Mondardini V."'

1. A decrease in the immunoglobulin G antibody response against the VlsE protein of Borrelia burgdorferi sensu lato correlates with the resolution of clinical signs in antibiotic-treated patients with early Lyme disease.

Author

Marangoni A, Sambri V, Accardo S, Cavrini F, Mondardini V, Moroni A, Storni E, and Cevenini R

Subjects
Adult, Aged, Female, Humans, Immunoglobulin G biosynthesis, Lyme Disease blood, Lyme Disease drug therapy, Male, Middle Aged, Retrospective Studies, Time Factors, Anti-Bacterial Agents therapeutic use, Antibodies, Bacterial blood, Antigens, Bacterial immunology, Bacterial Proteins immunology, Borrelia burgdorferi immunology, Immunoglobulin G blood, Immunosuppression Therapy, Lipoproteins immunology, Lyme Disease diagnosis, Lyme Disease immunology
Abstract

The purpose of this study was to evaluate the diagnostic performance of the LIAISON Borrelia Screen (Diasorin, Saluggia, Italy), a new automated immunoassay based on the chemiluminescent technology (chemiluminescence immunoassay). To assess whether a decrease in a negative value in the anti-VlsE immunoglobulin G (IgG) antibody titer was correlated with a positive response to treatment, a group of serially collected serum samples from 67 patients with culture-confirmed erythema migrans was retrospectively studied. All the patients had been treated with antibiotics and were free of disease within 3 to 6 months of follow-up. All the 15 patients who were found to be IgG positive at the time of enrollment and who were bled at least four times during the follow-up became IgG seronegative at 2 to 6 months posttreatment. These results indicate that a decline in the anti-VlsE antibody titer coincides with effective antimicrobial therapy in patients with early localized Lyme disease.

Published
2006
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2. Comparative evaluation of three different ELISA methods for the diagnosis of early culture-confirmed Lyme disease in Italy.

Author

Marangoni A, Sparacino M, Cavrini F, Storni E, Mondardini V, Sambri V, and Cevenini R

Subjects
Borrelia burgdorferi classification, Borrelia burgdorferi genetics, False Positive Reactions, Humans, Italy, Lyme Disease blood, Polymerase Chain Reaction methods, Reproducibility of Results, Sensitivity and Specificity, Borrelia burgdorferi isolation & purification, Enzyme-Linked Immunosorbent Assay methods, Lyme Disease diagnosis
Abstract

In this study the raising and development of the immune response to Borrelia burgdorferi infection in 45 Italian patients suffering from culture-confirmed Lyme borreliosis erythema migrans was investigated. A total of 95 serially collected serum samples were tested by using three different commercial ELISAs: recomWell Borrelia (Mikrogen), Enzygnost Borreliosis (DADE Behring) and Quick ELISA C6 Borrelia (Immunetics). The sensitivities of the ELISAs were as follows: Enzygnost Borreliosis IgM, 70.5 %; Quick ELISA C6 Borrelia, 62.1 %; recomWell Borrelia IgM, 55.7 %; recomWell Borrelia IgG, 57.9 %; and Enzygnost Borreliosis IgG, 36.8 %. In order to compare the specificity values of the three ELISAs, a panel of sera obtained from blood donors (210 samples coming from a non-endemic area and 24 samples from an endemic area) was tested, as well as sera from patients suffering from some of the most common biological conditions that could result in false-positive reactivity in Lyme disease serology (n = 40). RecomWell Borrelia IgG and recomWell Borrelia IgM were the most specific (97.1 % and 98.9 %, respectively), followed by Quick ELISA C6 Borrelia (96.7 %). Enzygnost Borreliosis IgG and IgM achieved 90.1 % and 92.3 % specificity, respectively. Sera that gave discrepant results when tested by the three ELISAs were further analysed by Western blotting.

Published
2005
Full Text
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5. A Decrease in the Immunoglobulin G Antibody Response against the VlsE Protein of Borrelia burgdorferi Sensu Lato Correlates with the Resolution of Clinical Signs in Antibiotic-Treated Patients with Early Lyme Disease

Author

Roberto Cevenini, Silvia Accardo, Vittorio Sambri, Valeria Mondardini, Alessandra Moroni, Francesca Cavrini, Elisa Storni, Antonella Marangoni, Marangoni A., Sambri V., Accardo S., Cavrini F., Mondardini V., Moroni A., Storni E., and Cevenini R.

Subjects
Adult, Male, Microbiology (medical), Time Factors, Lipoproteins, Clinical Biochemistry, Immunology, Biology, DIAGNOSIS, THERAPY, Immunoglobulin G, Lyme disease, Bacterial Proteins, Antigen, Borrelia, medicine, Humans, Immunology and Allergy, Borrelia burgdorferi, Aged, Retrospective Studies, Immunosuppression Therapy, Antigens, Bacterial, Lyme Disease, Antibody titer, Early Localized Lyme Disease, Middle Aged, medicine.disease, biology.organism_classification, Antibodies, Bacterial, Anti-Bacterial Agents, biology.protein, VLSE PROTEIN, ANTIBODY RESPONSE, Female, Microbial Immunology, Antibody
Abstract

The purpose of this study was to evaluate the diagnostic performance of the LIAISON Borrelia Screen (Diasorin, Saluggia, Italy), a new automated immunoassay based on the chemiluminescent technology (chemiluminescence immunoassay). To assess whether a decrease in a negative value in the anti-VlsE immunoglobulin G (IgG) antibody titer was correlated with a positive response to treatment, a group of serially collected serum samples from 67 patients with culture-confirmed erythema migrans was retrospectively studied. All the patients had been treated with antibiotics and were free of disease within 3 to 6 months of follow-up. All the 15 patients who were found to be IgG positive at the time of enrollment and who were bled at least four times during the follow-up became IgG seronegative at 2 to 6 months posttreatment. These results indicate that a decline in the anti-VlsE antibody titer coincides with effective antimicrobial therapy in patients with early localized Lyme disease.

Published
2006

6. Comparative evaluation of three different ELISA methods for the diagnosis of early culture-confirmed Lyme disease in Italy

Author

Vittorio Sambri, Antonella Marangoni, Roberto Cevenini, Elisa Storni, Monica Sparacino, Valeria Mondardini, Francesca Cavrini, MARANGONI A., SPARACINO M., CAVRINI F., STORNI E., MONDARDINI V., SAMBRI V., and CEVENINI R.

Subjects
Microbiology (medical), Enzyme-Linked Immunosorbent Assay, Microbiology, Polymerase Chain Reaction, Sensitivity and Specificity, Serology, Comparative evaluation, Lyme disease, Borrelia, medicine, Humans, False Positive Reactions, Borrelia burgdorferi, IMMUNOBLOTTING, Lyme Disease, biology, SEROLOGY, Borrelia Burgdorferi Infection, Endemic area, Reproducibility of Results, General Medicine, bacterial infections and mycoses, biology.organism_classification, medicine.disease, Serum samples, Virology, Italy, Immunology, ELISA
Abstract

In this study the raising and development of the immune response to Borrelia burgdorferi infection in 45 Italian patients suffering from culture-confirmed Lyme borreliosis erythema migrans was investigated. A total of 95 serially collected serum samples were tested by using three different commercial ELISAs: recomWell Borrelia (Mikrogen), Enzygnost Borreliosis (DADE Behring) and Quick ELISA C6 Borrelia (Immunetics). The sensitivities of the ELISAs were as follows: Enzygnost Borreliosis IgM, 70.5 %; Quick ELISA C6 Borrelia, 62.1 %; recomWell Borrelia IgM, 55.7 %; recomWell Borrelia IgG, 57.9 %; and Enzygnost Borreliosis IgG, 36.8 %. In order to compare the specificity values of the three ELISAs, a panel of sera obtained from blood donors (210 samples coming from a non-endemic area and 24 samples from an endemic area) was tested, as well as sera from patients suffering from some of the most common biological conditions that could result in false-positive reactivity in Lyme disease serology (n = 40). RecomWell Borrelia IgG and recomWell Borrelia IgM were the most specific (97.1 % and 98.9 %, respectively), followed by Quick ELISA C6 Borrelia (96.7 %). Enzygnost Borreliosis IgG and IgM achieved 90.1 % and 92.3 % specificity, respectively. Sera that gave discrepant results when tested by the three ELISAs were further analysed by Western blotting.

Published
2005

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