1. Beneficial nutraceutical modulation of cerebral erythropoietin expression and oxidative stress: an experimental study.
- Author
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Sedriep S, Xia X, Marotta F, Zhou L, Yadav H, Yang H, Soresi V, Catanzaro R, Zhong K, Polimeni A, and Chui DH
- Subjects
- Administration, Oral, Aging metabolism, Animals, Blotting, Western, Brain metabolism, Brain physiology, Erythropoietin blood, Male, Malondialdehyde analysis, Mice, Mice, Inbred BALB C, Models, Animal, Oxidative Stress drug effects, Phenethylamines pharmacology, Phycocyanin pharmacology, Phytochrome pharmacology, Receptors, Erythropoietin analysis, Sulfhydryl Compounds analysis, Up-Regulation, Antioxidants pharmacology, Brain drug effects, Dietary Supplements, Erythropoietin biosynthesis, Maze Learning drug effects, Memory drug effects, Receptors, Erythropoietin biosynthesis
- Abstract
The main object of this study is to examine the effect of Klamin®, a nutraceutical containing phenylethylamine, phycocyanins, mycosporine-like aminoacids and aphanizomenon flos aquae-phytochrome on the learning and memory ability, the oxidative status and cerebral erythropoietin and its receptor EPO/EPOR system in prematurely senescent (PS) mice. A total of 28 PS mice, selected according to a prior T-maze test, and 26 non-prematurely senescent mice (NPS) mice were chosen. PS animals were divided into 3 groups and followed for 4 weeks: A) normal chow diet; B) added with Klamin® at 20 mg/kg/day (low dose); C) added with Klamin® at 100mg/kg/day (high dose). A further group of NPS mice given either normal food (group D) or high dose Klamin® (group E) was also considered. The behavioral procedures of spatial learning ability (Morris test) showed that PS mice had significantly longer learning time as compared to their NPS counterpart (p<0.01), but this effect was prevented especially in mice supplemented with high-dose Klamin® (p<0.05) which improved performances in NPS mice (p<0.05). High-dose Klamin® supplementation restored the depleted total thiol concentration in the brain observed in PS mice while normalizing their increased malonildialdehyde level (p<0.05). Moreover, the high-dosage only caused a significant upregulation of EPO/EPOR system both in PS and in NPS animals (p<0.05). Taken together, these data suggest that this specific alga Klamath extract has considerable antioxidant and adaptogenic properties, also through a stimulatory effect of cerebral EPO/EPO system.
- Published
- 2011