1. Glycyrrhizin inhibits traumatic brain injury by reducing HMGB1-RAGE interaction.
- Author
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Okuma Y, Liu K, Wake H, Liu R, Nishimura Y, Hui Z, Teshigawara K, Haruma J, Yamamoto Y, Yamamoto H, Date I, Takahashi HK, Mori S, and Nishibori M
- Subjects
- Animals, Blood-Brain Barrier drug effects, Blood-Brain Barrier physiopathology, Brain drug effects, Brain pathology, Brain physiopathology, Brain Edema drug therapy, Brain Edema pathology, Brain Edema physiopathology, Brain Injuries pathology, Brain Injuries physiopathology, Capillary Permeability drug effects, Capillary Permeability physiology, Cognition drug effects, Cognition physiology, Disease Models, Animal, Dose-Response Relationship, Drug, Male, Mice, Knockout, Motor Activity drug effects, Motor Activity physiology, Random Allocation, Rats, Wistar, Receptor for Advanced Glycation End Products, Receptors, Immunologic genetics, Brain Injuries drug therapy, Glycyrrhizic Acid pharmacology, HMGB1 Protein metabolism, Neuroprotective Agents pharmacology, Receptors, Immunologic metabolism
- Abstract
Glycyrrhizin (GL) is a major constituent of licorice root and has been suggested to inhibit the release of high mobility group box-1 (HMGB1), a protein considered representative of damage-associated molecular patterns. We found that GL bound HMGB1 but not RAGE with a moderate equilibrium dissociation constant value based on surface plasmon resonance analysis. This complex formation prevented HMGB1 from binding to RAGE in vitro. The effects of glycyrrhizin on traumatic brain injury (TBI) induced by fluid percussion were examined in rats or mice in the present study. GL was administered intravenously after TBI. Treatment of rats with GL dose-dependently suppressed the increase in BBB permeability and impairment of motor functions, in association with the inhibition of HMGB1 translocation in neurons in injured sites. The beneficial effects of GL on motor and cognitive functions persisted for 7 days after injury. The expression of TNF-α, IL-1β and IL-6 in injured sites was completely inhibited by GL treatment. In RAGE-/- mice, the effects of GL were not observed. These results suggested that GL may be a novel therapeutic agent for TBI through its interference with HMGB1 and RAGE interaction., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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