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Your search keyword '"Lompo, Olga Melanie"' showing total 2 results
Start Over Author "Lompo, Olga Melanie" Topic brca1 protein
2 results on '"Lompo, Olga Melanie"'

1. Identification of BRCA1/2 p.Ser1613Gly, p.Pro871Leu, p.Lys1183Arg, p.Glu1038Gly, p.Ser1140Gly, p.Ala2466Val, p.His2440Arg variants in women under 45 years old with breast nodules suspected of having breast cancer in Burkina Faso.

Author

Sagna T, Bonora E, Ouedraogo MNL, Fusco D, Zoure AA, Bisseye C, Djigma F, Kafando JG, Zongo N, Douamba Z, Obiri-Yeboah D, Turchetti D, Pietra V, Lompo OM, Ouedraogo C, Seri M, and Simpore J

Subjects
Adult, Breast Neoplasms pathology, Burkina Faso, Female, Genetic Testing standards, Humans, Mammary Glands, Human pathology, Polymorphism, Single Nucleotide, BRCA1 Protein genetics, BRCA2 Protein genetics, Breast Neoplasms genetics, Mutation, Missense
Abstract

Breast cancer is the top cause of cancer mortality among women in the world and the second in Africa. The aims of this study were to: i) identify women with breast nodules suspected of having breast cancer ii) sequence the BRCA1 and BRCA2 genes and iii) screen mutations. From 2015 to 2016, 112 women aged from 35 to 44 years, who had come for consultation in the gynecology/obstetrics and the oncology department of the University Hospital Yalgado Ouedraogo, voluntarily agreed to participate to this study. Whole blood was collected from those with mammary nodules. The genomic DNA was extracted using Qiagen kit. FAST KAPA was used for genomic DNA amplification and the purified PCR products were analyzed by direct sequencing using Big Dye v1.1 and ABI 3730 automated sequencer. Nucleotides substitutions were determined. We identified BRCA1 SNPs rs1799966, rs799917, rs16942, rs16941, rs2227945, and BRCA2 SNPs rs169547, rs4986860. These identified variants are found mostly in cases of benign tumors of breast or ovarian cancer with familial history of breast cancer. This study in Burkina-Faso, is the basis for improved and more specific genetic testing, and suggests that additional genes contributing to an increased risk of breast cancer should be analyzed.

Published
2019
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2. Identification of BRCA1/2 p.Ser1613Gly, p.Pro871Leu, p.Lys1183Arg, p.Glu1038Gly, p.Ser1140Gly, p.Ala2466Val, p.His2440Arg variants in women under 45 years old with breast nodules suspected of having breast cancer in Burkina Faso

Author

Charlemagne Ouedraogo, Nayi Zongo, Jacques Gilbert Kafando, Elena Bonora, Jacques Simpore, Cyrille Bisseye, Florencia Djigma, Tani Sagna, Olga Melanie Lompo, Marie N. L. Ouedraogo, Zoenabo Douamba, Abdou Azaque Zoure, Daniela Fusco, Dorcas Obiri-Yeboah, Marco Seri, Daniela Turchetti, Virginio Pietra, Sagna, Tani, Bonora, Elena, Ouedraogo, Marie Nabonswindé Lamoussa, Fusco, Daniela, Zoure, Abdou Azaque, Bisseye, Cyrille, Djigma, Florencia, Kafando, Jacques Gilbert, Zongo, Nayi, Douamba, Zoenabo, Obiri-Yeboah, Dorca, Turchetti, Daniela, Pietra, Virginio, Lompo, Olga Melanie, Ouedraogo, Charlemagne, Seri, Marco, and Simpore, Jacques

Subjects
0301 basic medicine, Oncology, Adult, medicine.medical_specialty, QH301-705.5, BRCA, Mutation, Missense, Single-nucleotide polymorphism, Breast Neoplasms, Polymorphism, Single Nucleotide, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Breast cancer, Internal medicine, Burkina Faso, medicine, Humans, Genetic Testing, Biology (General), Polymorphism, skin and connective tissue diseases, Mammary Glands, Human, Gene, Genetic testing, BRCA2 Protein, medicine.diagnostic_test, business.industry, BRCA1 Protein, General Medicine, medicine.disease, University hospital, genomic DNA, 030104 developmental biology, 030220 oncology & carcinogenesis, Female, Ovarian cancer, business, Breast nodules, SNPs
Abstract

Breast cancer is the top cause of cancer mortality among women in the world and the second in Africa. The aims of this study were to: i) identify women with breast nodules suspected of having breast cancer ii) sequence the BRCA1 and BRCA2 genes and iii) screen mutations. From 2015 to 2016, 112 women aged from 35 to 44 years, who had come for consultation in the gynecology/obstetrics and the oncology department of the University Hospital Yalgado Ouedraogo, voluntarily agreed to participate to this study. Whole blood was collected from those with mammary nodules. The genomic DNA was extracted using Qiagen kit. FAST KAPA was used for genomic DNA amplification and the purified PCR products were analyzed by direct sequencing using Big Dye v1.1 and ABI 3730 automated sequencer. Nucleotides substitutions were determined. We identified BRCA1 SNPs rs1799966, rs799917, rs16942, rs16941, rs2227945, and BRCA2 SNPs rs169547, rs4986860. These identified variants are found mostly in cases of benign tumors of breast or ovarian cancer with familial history of breast cancer. This study in Burkina-Faso, is the basis for improved and more specific genetic testing, and suggests that additional genes contributing to an increased risk of breast cancer should be analyzed.

Published
2019

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