1. Nanogold Labels, Covalent Gold, and Enzyme Metallography as Components of Nanodevices.
- Author
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Powell, Richard D., Hainfeld, James F., Gutierrez, Edmund, Furuya, Frederic R., Joshi, Vishwas N., Liu, Wenqiu, and Takvorian, Peter M.
- Subjects
NANOPARTICLES ,GENE amplification ,POLYMERASE chain reaction ,BREAST cancer ,COMBINATORIAL chemistry - Abstract
Covalently linkable gold nanoparticles provide both highly sensitive and precise localization and detection of biological targets, and components that may be integrated into nanoscale devices with molecular precision to impart a variety of useful properties. The applications of the 1.4 nm Nanogold particle to biological staining and detection are described, and its applications to nanostructured materials and nanodevices are reviewed. In addition, a new method for depositing metal from solution using targeted enzyme probes, EnzMet (enzyme metallography) allows highly sensitive and specific detection of genetic and protein targets in cells and tissues, and has been used to develop an improved test for HER2 gene amplification in breast cancer. EnzMet also provides a novel method for immunoelectron microscopic staining, a reagent for highly sensitive blotting, and a method for target detection on conductive array biochips. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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