1. Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population.
- Author
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Wegman-Points LJ, Teoh-Fitzgerald ML, Mao G, Zhu Y, Fath MA, Spitz DR, and Domann FE
- Subjects
- Aldehyde Dehydrogenase 1 Family, Animals, Breast Neoplasms enzymology, Breast Neoplasms virology, Catalase pharmacology, Cell Line, Tumor, Cell Proliferation drug effects, Female, Humans, Mice, Mice, Nude, Neoplastic Stem Cells cytology, Neoplastic Stem Cells virology, Polyethylene Glycols pharmacology, Superoxide Dismutase pharmacology, Transplantation, Heterologous, Breast Neoplasms pathology, Isoenzymes metabolism, Neoplastic Stem Cells enzymology, Retinal Dehydrogenase metabolism, Retroviridae physiology
- Abstract
Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The retroviral induced increases in cancer cell growth rate were partially inhibited by treatment with 100 U/ml polyethylene glycol-conjugated-(PEG)-superoxide dismutase and/or PEG-catalase. These results show that retroviral infection of MDA-MB231 human breast cancer cells is capable of enhancing cell proliferation and cancer stem cell populations as well as suggesting that modulation of reactive oxygen species-induced pro-survival signaling pathways may be involved in these effects.
- Published
- 2014
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