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The aim of this study was to assess the effects of Saccharomyces cerevisiae supplementation on the metabolic profile of brood mares, raised in Tiaret Algeria. Ninety pregnant mares were used between 2013 and 2015. Animals were assigned to treatment’s group and to a control group, the mares were fed with corn, soybean meal, barley, wheat bran, and the second (yeast group) was supplemented with 10g alive yeast (Saccharomyces cerevisiae 47) daily from 300 Day of gestation to 30 days postfoaling. For biochemical analyses, jugular blood samples were collected. Each mare was collected five times, 30 days (F-30), 15 days (F-15) before foaling, at the foaling day (F), at 15 (F+15) and 30 days after foaling (F+30). to determine the following parameters, glucose, urea, creatinine, total proteins, cholesterol, triglyceride, albumin, Aspartate Amino-Transferase(AST), Alanine Amino-Transferase (ALT), Gamma-Glutamyl-Transferase (γ-GT), Calcium and inorganic phosphorus. In this study, the serum glucose mean values were significantly higher (p<0,05) at F+30 with 0,85±0,16 g/l in the treatment group against 0,73±0,09 g/l in the control one. In this study, urea serum levels at F-15 and at foaling were significantly lower (p<0,05) in the treatment group with 0,24±0,05 g/l and 0,27±0,05 g/l respectively against 0,33±0,10 g/l and 0,38±0,12 g/l respectively in the control one. This experiment showed that adding Saccharomyces cerevisiae to mare’s meal in the peripartum period enhanced the metabolism of glucose and proteins and decreased urea serum levels. [ABSTRACT FROM AUTHOR]