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The lamellation fractures in marine shales are important to the accumulation and preservation of shale gas, whose characteristics and controlling factors are still unclear. This paper focus on the characteristics descriptions and controlling factor analyses based on core observations, thin sections, and scanning electron microscope (SEM) experiments. The marine shales of Paleozoic Wufeng Formation and Longmaxi Formation in the Fuling Area of Eastern Sichuan Basin are taken as an instance. The lamellation fractures are occurring along the direction of the lamellations, partially of them curved, bifurcated, and converged. The apertures of fractures typically range from 1 to 500 μm. Only a few (<1%) lamellation fractures are filled with quartz, calcite, pyrite, and bitumen. After a series of analyses, developments of lamellation fractures are controlled by total organic carbon (TOC), brittle minerals, laminae, and pyrite. (1) The organic matters control the formation and evolution of lamellation fractures by generating gas and resulting in overpressure. An increase in the TOC content of 1% can increase the lamellation fracture density by 0.26 cm-1. (2) Brittle minerals, especially authigenic quartz, is also an important controlling factor to the development of lamellation fractures. When BI increases by 10%, the lamellation fracture density increases by 19%. (3) Besides, siliceous and carbonate laminae in the shale are more suitable for lamellation fractures to develop. When the laminae density is smaller than 4 cm−1, it is easier to develop lamellation fractures with higher laminae density. However, too densely-distributed laminae will suppress the formation of lamellation fractures. (4) Higher pyrite content is good for the development of lamellation fractures. Lamellation fracture density will increase by 9%, with pyrite content increased by 1%. For the shale at the bottom of the Wufeng Formation - Longmaxi Formation, lamellation fractures develop extensively due to the high TOC and pyrite content, great brittleness, and moderate laminae density. The density of lamellation fractures decreases from the bottom to the top. • Quantitative characterize about development characteristics of the lamellation fractures. • The lamellation fractures is controlled by brittle mineral, TOC, laminae and pyrite. • TOC is the main one that affects the development of lamellation fractures. • Clarify vertical distribution characteristic of lamellation fractures in the Wufeng-longmaxi Fm. [ABSTRACT FROM AUTHOR]