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Wheat bran, while a nutritious and economic feed ingredient, contents high levels of non-starch polysaccharides which entraps nutrients and interferes digestion and absorption. To study the influence of fermented wheat bran by xylanase-producing Bacillus cereus on growth performance and intestinal microflora of broiler chickens, a total of 180 broilers (21-day-old, mixed of male and female) were randomly divided into 3 treatments, with 6 replicates in each treatment and 10 broilers in each replicate: 1) control check (CK), corn-soybean meal-based diet; 2) wheat bran group (WB), 5% of the corn were replaced with wheat bran; and 3) fermented wheat bran group (FWB), 5% of the corn were replaced with fermented wheat bran. Growth performance was determined in the period of 21- to 42-day-old. Intestinal digestive enzyme activities and microbiota diversity were analyzed on day 42. No differences were observed on growth performance among treatments (P > 0.05). The activity of amylase in the duodenum of FWB was 1.56 times higher than CK (P < 0.05). The Chao1 index of microbiota in cecum of FWB increased 24.26% compared with CK (P < 0.01). The amount of Bifidobacteriaceae in cecum of WB was 29.1 times and 15.8 times higher than CK and FWB (P < 0.05) respectively. Principal co-ordinates analysis in cecum revealed the dissimilarity microbiota among treatments. In summary, the use of fermented wheat bran to partially replace corn (5%) in diets had no adverse effect on growth performance and triggered beneficial effects such as increasing duodenal amylase activity and intestinal microflora abundance in broiler chickens. These observations support that solid-state fermentation by xylanase-producing Bacillus cereus is feasible approach to pre-treat wheat bran for feedstuff industry. [ABSTRACT FROM AUTHOR]

The expression of genes related to the Toll-like receptors (TLRs) signaling pathway were determined. Group A, B and C fed with basal diet and group D, E and F induced TD by feeding a basal diet containing 100 mg·kg−1 thiram. rGSTA3 protein was injected at 20 μg·kg−1 in group B, E and at 50 μg·kg−1 in C, F. Results suggested that lameness and death of chondrocytes were significant on day 14. TLRs signaling pathway related genes were screened based on the transcriptome enrichment, and validated on qPCR. IL-7, TLR2, 3, 4, 5, 7, 15, MyD88, MHC-II, MDA5 and TRAF6 were significantly (p < 0.05) expressed in group E and F as compared to group D on day 14 and 23. IL-7, MHCII, TRAF6, TLR3, TLR5, TLR7, and TLR15 determined insignificant in group D compared to group A on day 23. TD occur in an early phase and alleviated in the later period. rGSTA3 protein can prevent apoptosis and repair degraded chondrocytes. Image 1 • TD is an important undetected avian growth plate disease. • Immune genes regulate apoptosis, ultimately death of chondrocytes occured. • Toll-like receptor (TLRs) mediates cellular immune responses to cause apoptosis. • rGSTA3 protein treatment prevents the apoptosis by downregulating TLRs on day 14 and day 23. [ABSTRACT FROM AUTHOR]