1. [THE PATHOGENIC POTENTIAL OF MORAXELLA CATARRHALIS AND STAPHYLOCOCCUS EPIDERMIDIS UNDER INFLAMMATORY PROCESSES OF UPPER RESPIRATORY TRACTS].
- Author
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Kraeva LA, Burgasova OA, Kunilova ES, Petrova IS, Tseneva GY, and Bespalova GL
- Subjects
- Bacterial Adhesion, Bacterial Typing Techniques, Bronchitis pathology, Ear, Middle microbiology, Ear, Middle pathology, Gene Expression, Humans, Moraxella catarrhalis genetics, Moraxella catarrhalis growth & development, Moraxella catarrhalis isolation & purification, Moraxellaceae Infections pathology, Otitis Media pathology, Pneumonia, Bacterial pathology, Polymerase Chain Reaction, Respiratory System microbiology, Respiratory System pathology, Sinusitis pathology, Staphylococcal Infections pathology, Staphylococcus epidermidis genetics, Staphylococcus epidermidis growth & development, Staphylococcus epidermidis isolation & purification, Virulence Factors genetics, Virulence Factors metabolism, beta-Lactamases genetics, beta-Lactamases metabolism, Bronchitis microbiology, Moraxella catarrhalis pathogenicity, Moraxellaceae Infections microbiology, Otitis Media microbiology, Pneumonia, Bacterial microbiology, Sinusitis microbiology, Staphylococcal Infections microbiology, Staphylococcus epidermidis pathogenicity
- Abstract
The frequent isolation from biological material of Moraxella catarrhalis under bronchitis and pneumonia and Staphilococcus epidermidis under rhinitis and sinusitis requires profound investigation offactors ofpathogenicity ofthe mentioned microorganisms. The genetic and phenotypic markers of virulence of strains M. catarrhalis and S. epidermidis are examined. Their etiologic role in development of infection processes of respiratory tract and middle ear is determined The most of M catarrhalis strains isolated under bronchitis and pneumonia have gene mcaP responsiblefor production ofprotein McaP that provides adhesion to epithelium cell of host and lipolitic activity of bacteria. The strains isolated from patients with pneumonia had the most adhesive activity. The cluster of genes ICA with leading role of gene icaA is responsible for for availability offactors of intercellular adhesion in Staphilococci strains. In the clinical samples from patients with sinusitis this gene is detected 5 times more frequently than from healthy individuals. In phenotypic tests, expression of gene icaA in S. epidermidis isolated from patients is three times higher than in strains isolated from healthy individuals. To establish etiologic role of M. catarrhalis and S. epidermidis and to develop tactic of therapy of patients with bronchitis, pneumonia and sinusitis complex approach is needed, including detection of genetic and phenotypic markers of virulence in isolated microorganisms.
- Published
- 2015