1. Rapid Propagation of Chirita ophiopogoides in Vitro.
- Author
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Chuanming FU, Kanghua XIAN, Jinxiang HE, Fengluan TANG, Yunping SHI, and Ningzhen HUANG
- Subjects
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PLANT micropropagation , *BUD development , *SEEDLINGS , *COMPOSITION of leaves , *BANANAS , *POTATOES , *ROOTING of plant cuttings - Abstract
A method for in vitro culture and rapid propagation of Chirita ophiopogoides was developed using leaves as explants in this study. The results indicated that the medium MS+6-BA 0.1 mg/L+NAA 0.1 mg/L was suitable for bud induc- tion and seedling regeneration from leaves in primary culture. The media MS+0.5 mg/L 6-BA+0.1 mg/L NAA+10% banana+5% potato and MS+0.5 mg/L 6-BA+0.5 mg/L NAA+2% banana were very suitable for callus multiplication and seedling hardening in subculture, and the proliferation coefficients were 7.9 and 5.6 per 60 d respectively. The optimal rooting medium was MS and the rooting rate was 100% on day 30 of culture. The rooted plantlets of C. ophiopogoides were transplanted in greenhouse with humus soil and 92.5% survived. Theoretically, using the rapid propagation system, about 20 176 seedlings can be reproduced from a sterile plantlet in a year. [ABSTRACT FROM AUTHOR]
- Published
- 2015