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The article deals with the results of using cultivated allofibroblasts in topical treatment of burn wounds. Allofibroblasts obtained from the derma of burnt individuals were transplanted to the burn wounds of 28 patients. In 12 among 15 patients (80%) with IIIa-IIIb degree burns transplantation induced epithelialization of the burns in 12.1 +/- 2.4 days. Thirteen patients had deep burns, only in 3 of them with a circumscribed burn area transplantation proved effective. Complications of transplantation were encountered in 5 patients--rejection of the transplant in 2 and its suppuration in 3 patients. Allofibroblast transplantation may be applied as an independent method of treatment in IIIa, b degree burns.

The authors discuss the first results of the use of cultivated human allofibroblasts in the treatment of persisting wounds of the donor areas in 13 patients with deep burns and cicatricial deformities caused by the burns. Transplantation of cell cultures led to complete epithelialization of the wounds in 6-10 days. Complication, suppuration of the grafted material, was recorded in one case. Reaction of graft rejection was not encountered. The authors believe that the effect of the transplantation is due to stimulation of the epithelialization processes by the cultivated fibroblasts.

The work discusses the results of experience of long-year's standing of the staff of the Vishnevsky Institute of Surgery in the creation of a biological bandage with the use of cultivated human skin cells. A graft of cultivated human allofibroblasts was used for closure of burn wounds for the first time. Its efficacy was studied in 86 cases of burns. The use of cultivated allofibroblasts is indicated in bordering III ab degree burns and sluggishly healing donor areas formed after autodermoplasty. Study of the efficacy of cultivated allofibroblasts in the treatment of deep burns in combination with dermoplasty with a 1:6 perforated skin autograft are continued. The low cost and technical simplicity of the method allow cultivated fibroblast grafts to be recommended for wide clinical practice.

An autoplasty method for treatment of burns was worked out by the authors. In lab conditions a monolayer of epithelium is grown out from a piece of owner skin. Its size is 10 times (and more) as big as the initial material. The article deals with the results of treatment of 12 patients who had the transplantation of monolayer epithelium. Indications and contraindications are enclosed. The article concludes by the appreciation of data which were obtained during this work.

This paper deals with the possibility of closing of burn surfaces of skin wounds with cultivation of patients' fibroblasts. Transplantation of cultured fibroblasts was performed in 11 patients with 30-75% of a body surface burned. The results obtained indicate a prospect of this method of burn treatment.

By means of electron-microscopic radioautography RNA synthesis in blood neutrophils of burn patients was studied. Activation by phagocytic subjects was found to increase overall level of RNA synthesis in blood neutrophils either by increasing the number of cells capable of synthesis, or increasing their number in line with the intensification of the process in each of these cells. Alteration of activation conditions influences the degree of RNA synthesis by both of these means. Wound neutrophils are activated cells with a high level of RNA synthesis and low compared to blood cells, level of protein synthesis and increased capability of adhesion as well. They contain in their numerous phagosomata tissue detritus more frequently than bacteria do. Wound neutrophils are subjected to rapid destruction; it is not possible to stimulate in them phagocytic activity by means of opsonized Pseudomonas aeruginosa.

Autoradiographic and electron-microscopic methods were used for the study of cultures of human skin epidermocytes in media with a low calcium content. It has been demonstrated that the culturing of epidermocytes in a medium containing 0.15 mM of calcium suppresses differentiation and stimulates division of cells. The considerable number of dividing cells in the cultures permits their use both for grafting on wound surfaces and further cultivation.