1. Combination of stromal vascular fraction and Ad-COMP-Ang1 gene therapy improves long-term therapeutic efficacy for diabetes-induced erectile dysfunction
- Author
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Xiong Wang, Hai-Rong Jin, Zhen-Lin Gao, Ji-Kan Ryu, Ping Li, Feng-Chan Han, Guo Nan Yin, Jun-Kyu Suh, Lin Wang, Lei Shi, Xiang-Nan Lin, and Yin-Chuan Jin
- Subjects
0301 basic medicine ,Male ,medicine.medical_specialty ,Combination therapy ,erectile dysfunction ,Urology ,Genetic enhancement ,neural cell regeneration ,Mesenchymal Stem Cell Transplantation ,Permeability ,Diabetes Mellitus, Experimental ,03 medical and health sciences ,Mice ,Diabetes mellitus ,medicine ,Angiopoietin-1 ,Animals ,business.industry ,Regeneration (biology) ,Penile Erection ,General Medicine ,Genetic Therapy ,Stromal vascular fraction ,medicine.disease ,stromal vascular fraction ,Endothelial stem cell ,030104 developmental biology ,medicine.anatomical_structure ,Erectile dysfunction ,Intercellular Junctions ,Original Article ,Pericyte ,Endothelium, Vascular ,business - Abstract
Men with diabetic erectile dysfunction (ED) respond poorly to the currently available oral phosphodiesterase-5 inhibitors. Therefore, functional therapies for diabetic ED are needed. Stromal vascular fraction (SVF) and the adenovirus-mediated cartilage oligomeric matrix angiopoietin-1 (Ad-COMP-Ang1) gene are known to play critical roles in penile erection. We previously reported that SVF and Ad-COMP-Ang1 have only a short-term effect in restoring erectile function. Further improvements to ED therapy are needed for long-lasting effects. In the present study, we aimed to test if the combination of SVF and Ad-COMP-Ang1 could extend the erection effect in diabetic ED. We found that the combination therapy showed a long-term effect in restoring erectile function through enhanced penile endothelial and neural cell regeneration. Combination therapy with SVF and Ad-COMP-Ang1 notably restored cavernous endothelial cell numbers, pericyte numbers, endothelial cell-cell junctions, decreased cavernous endothelial cell permeability, and promoted neural regeneration for at least 4 weeks in diabetic mice. In summary, this is an initial description of the long-term effect of combination therapy with SVF and Ad-COMP-Ang1 in restoring erectile function through a dual effect on endothelial and neural cell regeneration. Such combination therapy may have therapeutic potential for the treatment of diabetic ED.
- Published
- 2018