1. The stability of free and bound prostate-specific antigen
- Author
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Ian Eardley, Jon Cartledge, H Verril, P Clarkson, and D Thompson
- Subjects
Gynecology ,Analyte ,medicine.medical_specialty ,Chromatography ,business.industry ,Urology ,Free prostate specific antigen ,Whole blood sample ,Prostate-specific antigen ,Urology clinic ,Medicine ,Routine clinical practice ,business ,Total psa ,Whole blood - Abstract
OBJECTIVE To determine if the assay for free prostate specific antigen (fPSA) and the calculated ratio of fPSA to total PSA (f/tPSA) is stable in conditions likely to be met in routine clinical practice. MATERIALS AND METHODS Two blood samples were obtained from 27 patients attending a routine urology clinic. Sample 1 was centrifuged immediately, assayed for fPSA and tPSA, and the f/tPSA calculated. This sample was then stored at 4 degrees C for 24 h, 48 h and 1 week, or at -20 degrees C for 24 h, 1 week and 1 month before the assays for fPSA and tPSA were repeated. The second sample was left at room temperature for 24 h before assay and processing, as for sample 1. RESULTS tPSA is a highly stable analyte; if whole blood samples are processed immediately, fPSA is stable for 24 h at 4 degrees C and 1 month at -20 degrees C. There was a significant reduction in the calculated f/tPSA in samples stored for >/=24 h at 4 degrees C (P
- Published
- 2001
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