1. Recurrent KRAS, KIT and SF3B1 mutations in melanoma of the female genital tract
- Author
-
Yanping Chen, Long-feng Ke, Wen-wen Zhang, Yuan-jun Cai, and Jianping Lu
- Subjects
Adult ,0301 basic medicine ,Neuroblastoma RAS viral oncogene homolog ,Cancer Research ,Genital Neoplasms, Female ,PDGFRA ,medicine.disease_cause ,Vulva ,Proto-Oncogene Proteins p21(ras) ,Targeted therapy ,03 medical and health sciences ,0302 clinical medicine ,Female genital tract ,Surgical oncology ,Genetics ,medicine ,Humans ,Melanoma ,Cervix ,neoplasms ,RC254-282 ,Aged ,biology ,Malignant melanoma ,CD117 ,business.industry ,Research ,High-Throughput Nucleotide Sequencing ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Middle Aged ,Phosphoproteins ,medicine.disease ,digestive system diseases ,Proto-Oncogene Proteins c-kit ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,030220 oncology & carcinogenesis ,Mutation ,Cancer research ,biology.protein ,Driver genes ,Female ,RNA Splicing Factors ,KRAS ,business - Abstract
Background Malignant melanoma of the female genital tract is relatively uncommon and accounts for 3–7% of all melanoma localizations. This study aimed to identify driver genes in melanoma of the female genital tract with the purpose of enhancing understanding of disease pathogenesis and identifying potential new therapeutic targets to develop effective therapies. Methods KIT (CD117) and BRAF expression were detected immunohistochemically. Polymerase Chain Reaction (PCR) and Sanger sequencing techniques were performed to identify the mutational status of BRAF, NRAS, KRAS, NF1, KIT, PDGFRA and SF3B1 on 19 melanomas of the female genital tract, paired with 25 cutaneous melanomas, 18 acral melanomas and 11 melanomas of nasal cavity. Results Somatic variant analysis identified KRAS (6/19; 32%) as the most commonly mutated gene, followed by KIT (4/19; 21%), SF3B1 (3/19; 16%) and NRAS (1/19; 5%). None of the cases were found to harbor BRAF, NF1 and PDGFRA mutations in melanomas of the female genital tract. However, none of the cases were found to harbor SF3B1 and KIT mutations in cutaneous melanomas, acral melanomas and melanomas of nasal cavity. Recurrent KIT mutations, as well as mutations in the less frequently mutated genes NRAS and SF3B1, were exclusively detected in vulvovaginal melanomas, but not in tumors arising in the cervix. However, recurrent KRAS mutations were detected in similar frequencies in tumors of the vulva, vagina, and cervix. Additionally, recurrent KRAS and KIT mutations occurred predominantly in polygonal and epithelioid cell types of melanoma in the female genital tract. Immunohistochemistry revealed moderate or strong cytoplasmic CD117 expression in 6 of the 19 cases (31.6%). Conclusions We observed that gynecologic melanoma harbored distinct mutation rates in the KIT, BRAF, SF3B1, KRAS, and NRAS genes. Our findings support the notion that gynecologic melanoma is a distinct entity from non-gynecologic melanoma, and these findings offer insights into future therapeutic options for these patients.
- Published
- 2021