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Speed agility is considered as the whole assessment of speed of movement, agility and coordination. The 10x4m test has been broadly used to evaluate physical fitness and overall health in children ...

Purpose:To analyze the anthropometric and physiological characteristics of competitive 15- to 16-year-old young male road cyclists and scale them according to a dichotomous category of successful/unsuccessful riders.Methods:A total of 103 15- to 16-year-old male road cyclists competing in the Italian national under 17 category performed a laboratory incremental exercise test during the in-season period. Age, height, body mass, body mass index, peak height velocity, and absolute and relative power output at 2 mmol/L and 4 mmol/L of blood lactate concentration were compared between 2 subgroups, including those scoring at least 1 point (successful, n = 70) and those that did not score points (unsuccessful, n = 61) in the general season ranking.Results:Successful and unsuccessful riders did not differ anthropometrically. Successful riders recorded significantly higher absolute and relative power output at 2 mmol/L and 4 mmol/L of blood lactate concentration compared with unsuccessful riders. Successful riders were also significantly older and had advanced biological maturation compared with their unsuccessful counterparts.Conclusion:Power associated with blood lactate profiles, together with chronological age and peak height velocity, plays an important role in determining race results in under 17 road cycling. Physiological tests could be helpful for coaches to measure these performance predictors.

To examine associations between motor coordination, body mass index (BMI), and sports participation in children 6–11 years old. 240 primary school children were enrolled in this cross-sectional study, divided into three age groups, each of which was subdivided into four weight classes: underweight (UW), normoweight (NW), overweight (OW), and obese (OB). The UW and NW groups were then compared to the OW and OB groups for differences in motor coordination ability and sports participation. Motor coordination ability was observed to decrease and the proportion of OW/OB children to increase with age. A significant association between BMI and motor coordination ability was noted (p

Children with obesity tend to have lower level of physical activity compared to non-obese peers. In fact, sedentary behaviors are prevalent in obese children causing difficulties to perform motor tasks and engaging in sport activities. This, in turn, has direct repercussions on adiposity and related comorbidities. The aim of the study was to investigate several components of fitness and their relationship with the degree of fatness in children. We considered 485 Italian schoolchildren (9.5 ± 1.12 years). BMI and prediction modelling outputs of fat mass were employed as markers of body fatness. Physical fitness (PF) was assessed by the 9-item test battery (explosive power, leg muscle power, arm muscle power, upper body power, coordination, agility, speed and endurance). Differences between groups in the PF tests (p &lt, 0.05) were noted. A similar pattern was reflected in both genders. The relationship between anthropometrics’ characteristics and PF tests showed that weight and fat mass had a high level of correlation with different PF tests. Our findings highlight the importance of investigating the degree of fatness in relation with different components of fitness, in children and adolescents. This combination of proxies may cover an unexpectedly helpful screening of the youth population, for both health and performance.

BACKGROUND: Italy early experienced one of the most suffering impact of the COVID-19 outbreak in terms of number of cases and death toll. Progressively, more compelling national restrictions hardened citizens’ lifestyle and habits, including limiting mobility and outdoor physical activity (PA). In a second phase, people were allowed to perform outdoor activities close to their houses and thereafter motor or sports activity were gradually reintroduced. These drastic changes raised the question on how to cope and exploit the residual opportunities of PA under circumstances of home confinement. METHODS: International Physical Activity Questionnaires were administered to 1568 youngsters from North-western Italian high schools, before, during, and after lockdown enacted by Italian Government to contrast the COVID-19 pandemic. RESULTS: Student’s PA levels were significantly different before (1676.37±20.6 MET-min/week) and after (1774.50±33.93 MET-min/week) the governmental restrictions (TIME effect: F=3.49; ηp2=0.005 P=0.03). There was a significant TIME*CATEGORY interaction effect (F=8.37; ηp2=0.021; P2520 MET-min/week) increased their PA during (3467.48±55.85 MET-min/week) and after (3515.73±65.75 MET-min/week) the lockdown measures with respect to their baseline levels (3151.43±42.41 MET-min/week). CONCLUSIONS: Lockdown measures, including isolation, impacted negatively on the PA levels of the already inactive or moderately active young population. Government actions should enhance strategies to control physical inactivity during pandemics like COVID-19.

Background. Adolescent idiopathic scoliosis (AIS) is included into the category of pathologies that could affect postural control. Rarely AIS shows symptoms but often compromises the normal positioning of the head, trunk and, more generally, of the limbs in the space. We used a stabilometric platform to evaluate the motor control outcomes during a self-elongation in girls with AIS. Methods. In 10 girls with AIS, we evaluated the center of pressure (COP) modifications on a baropodometric platform in a standing position and after a self-elongation correction. Results. All the outcomes (except the eccentricity) showed an increasement during the self-elongation exercise even if the significant differences were not found. Conclusion. SE correction contributes to ameliorate the COP symmetry with a better repercussion on the balance management. This highlights the importance of repetitions during all activities of daily life.

Obesity has been associated with several alterations that could limit physical activity (PA) practice. In pediatrics, some studies have highlighted the importance of enjoyment as a motivation to begin and maintain adherence in PA. Since self-reported physical (SRPF) fitness was related to motivation, the aim of this study was to investigate the existence of differences between SRPF in children with obesity (OB) compared to normal weight (NW). The International Fitness Enjoyment Scale (IFIS) questionnaire was administered to 200 OB and 200 NW children. In all the subjects, height, weight, and BMI and in OB children adiposity indexes including waist circumference (WC), body shape index (ABSI), triponderal mass index (TMI), and fat mass were measured. NW group showed higher IFIS item scores than the OB group (p &lt, 0.01), except in muscular strength. In OB, the anthropometric outcomes were inversely correlated to SRPF outcome except for muscular strength. OB children reported a lower perception of fitness that could limit participation in PA/exercise programs. The evaluation of anthropometric patterns may be useful to prescribe a tailored exercise program considering individual better self-perception outcomes to obtain an optimal PA adherence.

The goal of our study was to examine the muscle activity of the shoulder girdle after isokinetic fatigue, which may simulate muscle activities commonly occurring during specific sport-related activities in recreational overhead asymptomatic athletes. We hypothesized that exercise-induced fatigue, reported after isokinetic protocols, may cause a decrease in the median frequency (MF) of the upper trapezius (UT), infraspinatus (IS), and deltoid muscles. Twenty-four male overhead volleyball (n = 8), handball (n = 8), and tennis (n = 8) athletes participated in this study. All subjects were without shoulder injury history. The surface electromyography (SEMG) was collected on the right (dominant) side of the shoulder girdle muscles in the following order: UT, IS and anterior (DA), and posterior deltoideus (DP). The fatigue protocol consisted of three sets of 32 maximum isokinetic concentric contractions while performing shoulder internal and external rotation at an isokinetic speed of 120 o/s. The resultant difference in median frequency (ΔMF) values consistently dropped after the fatiguing tasks across all recorded muscles, in terms of the initial MF (MFINI = 65.1 ± 1.1 Hz) and final MF (MFFIN = 57.9 ± 0.9 Hz), and the main effect of time was significant (F(1,22) = 43.15, p &lt, 0.001). MF values decreased mostly for IS (ΔMFIS = −9.9 ± 1.6 Hz) and DP (ΔMFPD = −9.5 ± 1.9 Hz) muscles, while DA and UT showed smaller changes (ΔMFDA = −6.9 ± 1.5 Hz) and (ΔMFUT = −3.2 ± 1.3 Hz). The results of our study show a meaningful contribution in determining increased fatigue of the shoulder girdle muscles during repeated isokinetic internal-external rotation protocols. We have also demonstrated a significant decrease in MF in all examined muscles, especially IS and DA.

In this paper we present a device integrating on a single glass substrate a transparent heater and two resistive temperature sensors. Both heater and sensors are constituted by ITO/Au/ITO stacked film, whose thicknesses have been optimized in order to achieve at the same time good electrical conductivity (about 106 S/m) and high transparency degree (higher than 80%) in the visible region of the spectrum. A double-spiral design has been chosen for the heater in order to achieve a uniform temperature distribution on a large area. Indeed, this geometry ensures a temperature variation of ±1.4 °C in a 2.8 cm diameter circle. For the resistive temperature sensors, a distributed meander-shaped geometry ensures temperature sensitivity as high as 0.12 $\text{k}\pmb \Omega / ^{\circ }\text{C}$ . The proposed device shows therefore the capability to work as a large-area transparent heater introducing the possibility to perform a precise control of the substrate temperature.

This work reports on the development of a large-area thin film heater for lab-on chip applications. The device is a chromium/aluminum/chromium stacked structure with a spiral shape fabricated on a $5\times 5\text{cm}^{2}$ glass substrate through microelectronic technologies. Its geometry has been designed and optimized by COMSOL Multiphysics in terms of pitch and line width to achieve a uniform temperature distribution. Measurements performed with a thermo-camera show temperature variation below 1.5°C over 5cm2 area in excellent agreement with numerical modeling. To our knowledge, this is the largest area with such temperature uniformity achieved with a single heater in lab-on-chip systems. This opens the route for large-area thermal treatments as multiple-well DNA amplification or cell culture.

Peripheral arterial occlusive disease (PAOD) limits walking efficiency and distance. The main disabling symptom is vasculopathy that leads to claudicatio intermittens and limits walking efficiency. Stretching techniques are effective in treatments for retractions and are used to improve flexibility of triceps surae and range of motion of the ankle. The aim of this study is to evaluate the effects of a combined walking and stretching program on walking efficiency in elderly PAOD patients. Seventy patients with PAOD stage II of the Leriche&ndash, Fontaine classification were randomly assigned into a stretching group (SG) or conditioning group (CG). Both groups participated in an eleven-day intensive rehabilitation program based on walking and strength training, while the SG performed five extra sessions of stretching. Walking autonomy was assessed through treadmill and overground tests with the recording of initial and absolute pain. A univariate ANOVA analysis was applied for the differences between the initial and final outcomes. Walking autonomy improved in both groups (p &lt, 0.01), while only the SG improved flexibility (from &minus, 14.0 &plusmn, 8.1 to &minus, 10.3 &plusmn, 8.3 cm, p &lt, 0.01). An intensive eleven-day rehabilitation program based on walking, strength, and stretching exercises is effective to improve the onset and the delay of pain during walking in patients with PAOD.

Lower limb explosive strength has been widely used to evaluate physical fitness and general health in children. A plethora of studies have scoped the practicality of the standing broad jump (SBJ), though without accounting for body dimensions, which are tremendously affected by growth. This study aimed at modeling SBJ-specific allometric equations, underlying an objectively predictive approach while controlling for maturity offset (MO). A total of 7317 children (8&ndash, 11 years) were tested for their SBJs, demographics and anthropometrics data were also collected. The multiplicative model with allometric body size components, MO, and categorial differences were implemented with SBJ performance. The log-multiplicative model suggested that the optimal body shape associated with SBJs is ectomorphic (H = &minus, 0.435, M = 1.152). Likewise, age, sex, and age&ndash, sex interactions were revealed to be significant (p &lt, 0.001). Our results confirmed the efficacy of the allometric approach to identify the most appropriate body size and shape in children. Males, as they mature, did not significantly augment their performances, whereas females did, outperforming their peers. The model successfully fit the equation for SBJ performance, adjusted for age, sex, and MO. Predictive equations modeled on developmental factors are needed to interpret appropriately the performances that are used to evaluate physical fitness.

Highlights • An adequate Physical Activity level can help patients to maintain good glycemia control. • People with type 1 diabetes reduced their Physical Activity level during quarantine. • People with type 1 diabetes increased mean glycemic values. • Specific suggestions on home training manage should be given to people with type 1 diabetes., Aims Coronavirus disease (Covid-19) could lead persons with pre-existing medical conditions to severe respiratory infections. The Italian Government introduced quarantine to limit viral transmission. This measure could lead people with type 1 diabetes (PWT1D) to disrupt daily care routine including PA practice with difficulties in glycemia management. This study aims to explore PA level in PWT1D before and during quarantine and to describe variation in glycemia values. Methods An online survey investigating medical factors and the perceived and PA level in pre-established period before and after the introduction of quarantine was developed. Comparison between pre and post quarantine was assessed by Wilcoxon Signed Ranks test for continuous variables. Results A total of 154 subjects satisfied the eligibility criteria (54.5% males, 44.8±12.5 years). We found a decrease of PA level (Godin Scale Score 25±1.7vs38.6±1.7 points), steps number and minutes of exercise (respectively 12.606±5026vs4.760±3.145 and 66±4vs38±3) and an increase of glycemia values (142.1± 25.4 mg/dLvs150.8±29.4 mg/dL) Conclusions PWT1D reported a decrease in exercise and worst glycemia. Although PWT1D tried to remain active, their PA level was inadequate to prevent glycemia rising. The difficult to maintain a glycemic control could expose patients to diabetes complications and to an higher risk to counteract infections.

Background The cardiorespiratory fitness (CRF) is one of the most important indicators of the health-related physical fitness components because it reflects the efficiency of the cardiovascular and respiratory systems. To indirectly assess CRF, the original protocol of the 20mSRT was adapted in a new version suitable for children called 20mSRT-PREFIT, providing performances comparable to the standards of the European area. In fact, CRF normative values are missing in the Italian context and therefore they would be meaningful to be assessed. We aimed to determine CRF from 20mSRT-PREFIT in a small sample of Italian pre-school children and to compare the outcomes with the available European references. Methods A convenience sample of 5-year-old pre-school children (N.=32, M/F ratio: 19/13) was freely recruited from a kindergarten of the northwest Italy. Children performed the 20mSRT-PREFIT and the number of full shuttles, exhaustion time, and maximum speed were collected to obtain CRF. Results Predicted VO2max was similar between Italian male and female groups. All other outcomes originating from the 20mSRT-PREFIT were consistent with those included in the available European databases. Conclusions According to these preliminary indications, Italian pre-school children may have a CRF level equivalent to that one ascertained in other European countries. Thus, current rakings should be cautiously scrutinized, as they might inappropriately underrate Italian children's performances.

The purpose of the study was to identify the optimal body shape and composition associated with physical fitness levels of children living in urban and rural areas of Italy. A total of 7102 children (11–14 years) were assessed for weight, height, percentage body fat (FM%), sit-and-reach flexibility (SAR), standing broad jump (SBJ) and sit-ups (SUP). A multiplicative allometric model, Y = a · massk1 · heightk2 ·e, was used to predict the physical outcome variables Y = SBJ and SUP. The model was expanded to incorporate FM% and SAR as follows Y = a · massk1 · heightk2 · FM%k3 · exp(b· FM% + c· SAR) ·e. Note that FM% was incorporated as a “gamma function” that allows an initial growth, and subsequent decline in Y as FM% increases in size. Although having an ectomorph body shape appears advantageous, being too thin appears detrimental to the strength outcomes. Being flexible would also benefit physical fitness levels. Finally, our results indicate that ursban children aged 11–14 have superior strength ...

Alterations of scapular kinematics affect the whole kinematic chain, potentially leading to the impingement syndrome. This is crucial in overhead sports, where athletes perform frequent and quick upper limb actions. In this manuscript, we aimed to assess the extent to which fatigue alters scapulo-thoracic and scapulo-humeral ranges of motion (RoM), as well as scapulo-humeral movement onset during different upper limb actions. Twenty-four young healthy males aged 22 ± 2 years (height: 1.82 ± 0.06 m, body mass: 78.0 ± 7.8 kg) performed three movements (upper limb elevation, scapular-plane abduction, and intra-extra rotation) before and after an isokinetic fatigue protocol (upper limb intra/extra rotation, 32 repetitions at 120 degrees/s). Pre vs. post fatigue RoM of humeral elevation and rotation, scapular retraction/protraction, and rotation and tilt were computed. Humerus-scapula movement delay was also determined. Humerus elevation range reduced during intra/extra humerus rotation in fatigued conditions (p = 0.006). Scapular tilt RoM increased after the fatigue protocol (p = 0.063, large effect). Humerus-scapular movement onset delay reduced in fatigued conditions of about 80 ms (p < 0.001, large effect). In sum, fatigued intra/extra upper limb rotators altered the scapulohumeral rhythm, and joints RoM in movements outside the scapular plane. Rather, movements close to the scapular plane were less prone to fatigue-induced alterations.

This paper presents the development of a lab-on-chip, based on thin-film sensors, suitable for DNA treatments. In particular, the system performs on-chip DNA amplification and separation of double-strand DNA into single-strand DNA, combining a polydimethylsiloxane microfluidic network, thin-film electronic devices, and surface chemistry. Both the analytical procedures rely on the integration on the same glass substrate of thin-film metal heaters and amorphous silicon temperature sensors to achieve a uniform temperature distribution (within ±1 °C) in the heated area and a precise temperature control (within ±0.5 °C). The DNA separation also counts on the binding between biotinylated dsDNA and a layer of streptavidin immobilized into a microfluidic channel through polymer-brushes-based layer. This approach results in a fast and low reagents consumption system. The tested DNA treatments can be applied for carrying out the on-chip systematic evolution of ligands by exponential enrichment process, a chemistry technique for the selection of aptamers.

The juggling action of six experts and six intermediates jugglers was recorded with a motion capture system and decomposed into its fundamental components through Principal Component Analysis. The aim was to quantify trends in movement dimensionality, multi-segmental patterns and rhythmicity as a function of proficiency level and task complexity. Dimensionality was quantified in terms of Residual Variance, while the Relative Amplitude was introduced to account for individual differences in movement components. We observed that: experience-related modifications in multi-segmental actions exist, such as the progressive reduction of error-correction movements, especially in complex task condition. The systematic identification of motor patterns sensitive to the acquisition of specific experience could accelerate the learning process.

The study and quantification of the effects of the space environment on human body is a primary task for future manned deep space missions. The risk models for radiation exposures incurred by astronauts beyond low-Earth orbit, have different limitations due to the difficulty to have terrestrial parallels on which to base risk estimates. Indeed, no terrestrial sources fully reproduce the deep space energy spectrum and the multi directional flux of the cosmic radiation. In situ analysis would therefore be fundamental in order to enable reliable studies about the effects of the radiation environment on living organisms as well as to evaluate customized radiological countermeasures for astronauts. A micro-incubator suitable for cubesat missions for studying in situ the effects of the space environment on cellular cultures is presented. The device is based on lab-on-chip technology with integrated thin-film sensors and actuators for the active control of the environmental conditions of the cell culture and for the monitoring of its metabolic status. In particular, the device includes an incubation chamber connected to a microfluidic network for the supply of nutrients and/or pharmaceuticals. A second network is used for the distribution of carbon dioxide through a thin gas-permeable membrane. On-chip on-demand production of carbon dioxide can be eventually achieved from the pyrolysis of sodium bicarbonate stored in a separate reservoir with a dedicated thin film heater. The same network can be used to supply a controlled atmosphere from a pressurized tank. The on-chip hydrogenated amorphous silicon photodiodes are used to measure the light emitted by genetically-modified cell cultures that express a bio-luminescent behavior when subjected to given stress conditions. Accurate temperature control is achieved by means of additional on-chip thin-film diodes and a transparent indium-tin-oxide heater located beneath the incubation chamber. From technological point of view, the system relies on the combination of different thin- and thick-film fabrication technologies jointly used with the aim to achieve a compact, automated and low-power device that represents a viable solution for biological experiments aboard cubesat satellites.

This work reports on the characterization of stability of amorphous silicon diodes used as temperature sensors in lab-on-chip systems. We found that under constant forward current injection, the voltage drop over the diode changes depending on the values of current and injection time. The optimized operating conditions for practical applications have been established on the base of the obtained experimental data.

This paper focuses on applications and electrical valorisation of microbial fuel cells (MFCs), a promising energy harvesting technique, suitable as clean power source to supply low power devices in wireless sensor networks (WSN) for environmental and agricultural monitoring. An MFC is a bioreactor that converts energy stored in chemical bonds of organic matter into electrical energy, through a series of reactions catalysed by microorganisms. An MFC can operate as bioreactor, as bioremediator and as biosensor. In the past decade, the evolution of low power electronics has made MFCs technology more attractive, because it has become suitable for low-power devices forming complete systems, such as the nodes of a WSN. Moreover, MFCs gained more interest because they can generate electric power while treating wastes. Unlike other fuel cells, MFCs can continuously generate clean energy at normal temperature and atmospheric pressure without any supplementary maintenance. Additionally, MFC may be used directly as a biosensor to analyse parameters like pH and temperature or arranged in the form of a cluster of devices to be use as a small power plant. A series of test was performed for the electrical valorisation of reactors as well as biosensor issues.

This work presents an equivalent electrical circuit of hydrogenated amorphous silicon diodes. It is constituted by four diodes and two resistances. Each element is directly related to the physical behavior of the thin film structure and models the different conduction regimes of the device.Results show a very good fitting of the experimental current-voltage characteristic up to several hundreds of millivolts in both forward and reverse bias conditions and demonstrate the suitability of the developed model for designing the diode fabrication parameters for specific application.

This work presents a bio-microsystem, based on thin film optoelectronic devices, for on-chip detection of fluorescence-based aptamer assay. The detection is achieved integrating on a single glass substrate amorphous silicon photosensors and a thin film interferential filter, while the aptamer-based biomolecular recognition is carried out in a microfluidic network functionalized with polymer brushes. To test the developed platform, aptamer 1.12.2 having high affinity toward Ochratoxin A molecules has been used in combination with the ruthenium complex [Ru(phen) 2 (dppz)]2+ intercalating dye. We found that the developed platform can perform the analysis by using a small volume of sample (about 10 µL) in short time (5 min) with a limit of detection equal to 1.56 ng/ mL. With respect to the existing literature, the reported system features compactness, high sensitivity and high versatility since the fluorophore-aptamer system may be used with different kind of analytes.

Regular participation in strength and conditioning activities positively correlates with health-related benefits in sports (team and individual). Maturity offset (MO) is a recognized parameter in fitness outcome assessment. The aims of the present study are to analyze cross-sectional allometric development of motor performances in a sample of adolescents and relate scaled motor performance to the estimated amount and type of physical activity and biological maturity status in 771 subjects aged 14–19 years. Three physical fitness components were evaluated using field tests (standing broad jump, sit-ups, shuttle run). Extra hours of sport after school (EHS) and MO were the covariates. The model to predict the physical performance variables was: Y = a · Mk1· Hk2· WCk3· exp(b · EHS + c · MO) · ε. Results suggest that having controlled for body size and body shape, performing EHS and being an early developer (identified by a positive MO slope parameter) benefits children in physical fitness and motor performance tasks.

This study aimed to investigate the visual biofeedback effect of a sensorized system for plantar pressure dynamic evaluation of in patients with a total hip replacement. Experimental group followed the rehabilitation training wearing sensorized insoles that provided images on three monitors. The control group followed the verbal instructions of physiotherapists during training. Weight bearing percentage healthy limb (WBPH), weight bearing percentage surgical limb (WBPS), swing healthy limb (SWH) and swing surgical limb (SWS) improved significantly more in the experimental group. The results underline the effectiveness of visual biofeedback based on sensorized system with dynamic evaluation of the plantar pressure.

The integration of systems for real-time monitoring of DNA amplification reactions is rather limited, because the heating system, the temperature control and the detection system are usually realized with separate modules. In this work, a lab-on-a-chip system for real-time monitoring of the multiple displacement amplification reaction (MDA) is presented. The amplification and detection unit consists of a system-on-glass (SoG) coupled to a microfluidic chip. The SoG includes thin film metallic resistors and amorphous silicon temperature sensors to control the temperature, as well as amorphous silicon photosensors and an interference filter for fluorescence detection on a single glass plate. The amplification reaction is carried out in the microfluidic chip made of cyclic olefin copolymer (COC). By using this setup, the multiple displacement amplification reaction can be monitored in real-time using the fluorophore [Ru(phen)2(dppz)]2+.

Background Several studies have assessed the fitness level of students to evaluate physical condition, compare groups with differences in social disadvantage and to define indexes for post training programs. Often, these large surveys compared groups without normative value that could define the real, practical gaps. Thus, the aim of this study was the definition of baseline values describing the fitness level of sedentary European students. Methods Standing broad jump (SBJ), sit up (SUP) and sit and reach (SAR) physical fitness tests were assessed on 31,476 students (age=11-13) from 6 European countries were collected and analyzed. Results The effect size for multiple groups ANOVA was obtained to verify the biological consistence of differences. Then, weighted-means were calculated and stratified for age and gender. Overall, boys obtained SAR scores close to zero, while girls obtained results between 2 and 7 cm. The SUP test revealed similar results between boys and girls (about 20). SBJ performances were similar among countries (over 160 cm for boys and 150 for girls). Conclusions Our results were aligned to other studies performed in other Continents or Countries and can contribute to the establishment of a large and objective reference to readily evaluate the physical fitness and health-related status of young students.

This paper focuses on microbial fuel cell (MFC) waste valorization, electricity generation and practical applications. A custom electronic analyzer dedicated to MFCs is presented. The measuring system allows to set up a complete electrical characterization of a microbial fuel cell, useful to facilitate and accurately analyze the charge and discharge phase of an MFC. Moreover, it allows power performance analysis and measurement of any kind of MFC typology, using a custom software, which automatically sets up a series of tests over a long period of time. Electronics design and a flow diagram of the measuring instrument project are provided. MFCs are a zero-emission energy harvesting technology: a bioreactor that converts energy stored in chemical bonds of organic compounds into electrical energy. Due to these properties, MFCs could be useful to a wide range of applications in the context of bio-reactions, bio-remediation or bio-sensing.

This paper reports the development of a miniaturized lab-on-glass,suitable for the on-chip detection of living cell bioluminescence and their on-chip thermal treatments. The glass substrate hosts, on one side, hydrogenated amorphous silicon diodes, working as both temperature sensors and photosensors, and, on the other side, transparent thin films acting as heating sources. The main challenge of the work is the determination of the correct fabrication recipes in order to satisfy the compatibility of different microelectronic steps. The measured uniformity of temperature distribution, sensitivity of the temperature sensors, reverse dark current and spectral response of the photosensors demonstrate the successful technological integration and the suitability of the developed lab-on-glass to control the cell temperature and detect the BL emission with high sensitivity.

This work reports on design and fabrication of a compact lab-on-chip system, based on detection of electrochemiluminescence (ECL) through thin film sensors. The proposed system couples an optoelectronic platform to a disposable microfluidic chip. The optoelectronic platform includes amorphous silicon photosensors for detection of ECL, while the microfluidic chip contains the ECL electrodes and the biological solutions to be analyzed. Optoelectronic characterization of photosensors and preliminary test on the ECL electrodes by cycling voltammetry show the suitability of these devices for the achievement of low-noise and high sensitive system.

Lab-on-chip are analytical systems which, compared to traditional methods, offer significant reduction of sample, reagent, energy consumption and waste production. Within this framework, we report on the development and testing of an optoelectronic platform suitable for the on-chip detection of fluorescent molecules. The platform combines on a single glass substrate hydrogenated amorphous silicon photosensors and a long pass interferential filter. The design of the optoelectronic components has been carried out taking into account the spectral properties of the selected fluorescent molecule. We have chosen the [Ru(phen)2(dppz)]2+ which exhibits a high fluorescence when it is complexed with nucleic acids in double helix. The on-glass optoelectronic platform, coupled with a microfluidic network, has been tested in detection of double-stranded DNA (dsDNA) reaching a detection limit as low as 10 ng/µL.

This work presents the development and testing of an integrated system for on-chip detection of thermochemiluminescent biomolecules. The activation energy of the reaction is provided by a transparent structure of thin film heaters deposited on one side of a glass substrate. Light, passing through the substrate, reaches an array of amorphous silicon differential structure deposited on the opposite side of the glass substrate. The structure is designed to perform differential current measurements between a light- shielded diode, whose current is sensitive only to temperature, and a photosensor, sensitive to both incident light and temperature. The device therefore balances the thermal variations of the photodiode current and reduces the dark-current noise. These features make the presented system very appealing as highly miniaturized micro-analytical devices for biosensing applications.

Background Many studies have reported negative association between fitness level and BMI categories but the lack of body weight correction and the systematic use of physical endurance test made these differences controversial. Thus, the aim of this study was the assessment of physical fitness level associated to BMI using alternative tests. Methods BMI was calculated as body mass/stature2 while fitness level was assessed using field test. In particular, Sit and Reach (SAR), Standing Broad Jump (SBJ), Shuttle Run Test 5 mx 10 (SHR), Sit ups (SUP), Bent arm hang (BAH) were assessed in 2545 students. Subsequently, normal weight/overweight/obesity/underweight/thinness students were classified according to the cut-off points defined in literature and then the relative fitness results. Results The performances in SBJ showed very low differences between BMI categories such as for SUP test. The effects size in SHR were low or close to moderate while in BAH thin students revealed high performance than normal/overweight peers. In SAR test no clear trends in the BMI categories were observed. Conclusions All test (exluding BAH) were similar for normal, overweight and thin students. This finding can be useful to teachers to encourage over/under-weighted students to adopt active life style because they are close to normal weight counterparts.

This paper presents the development of a compact optoelectronic device suitable for on-chip detection of fluorescent molecules. In order to obtain a highly integrated device, a long-pass multi-dielectric filter has been integrated with thin-film amorphous silicon photosensors on a single glass substrate. Filter rejects the excitation light, allowing the reduction of the distance between the source and the fluorescent site and avoiding the use of external optical component. The compatibility of the technological processes determined the materials and the temporal sequence of the device fabrication. The developed device has been designed for the fluorescence detection of ruthenium complex based molecules and tested, as a proof of concept, for the detection of double-stranded DNA down to 0.5 ng. Results demonstrate the correct operation of the integrated system in both rejecting the excitation light and in detecting the fluorescent signal, demonstrating the suitability of this optoelectronic platform in practical biomedical applications.

In this paper, we present an optoelectronic system-on-glass (SoG), suitable for detection of fluorescent molecule. It integrates, on the same glass substrate, an array of amorphous silicon (a-Si:H) photosensors and a thin film interferential filter. The system can be directly coupled with another glass substrate hosting a polydimethylsiloxane based microfluidic network where the fluorescent phenomena occur. The compatibility of the different technological steps to attain on the same glass substrate the photosensors and the filter determined the sequence, the selection of materials and the deposition parameters of the whole process. The electro-optical characterization of the photodiode, performed after the filter deposition, demonstrated the efficacy of the filter in reducing the excitation light. The system has been successfully tested using the ruthenium complex [Ru(phen)2(dppz)]2+, a fluorescent dye which works as DNA intercalating molecule. © Springer International Publishing AG 2018.

The present paper describes the development of an integrated lab-on-chip, in which viral DNA amplification with real-time on-chip detection is carried out under constant temperature of 65 °C. The lab-on-chip is composed of a disposable 10-μL polydimethylsiloxane reaction chamber which is thermally and optically coupled to a glass substrate that hosts a thin-film metallic resistive heater and thin-film amorphous silicon diodes which act as temperature and radiation sensors. A loop-mediated isothermal amplification (LAMP) technique was optimized to specifically amplify parvovirus B19 DNA and coupled with Bioluminescent Assay in Real Time (BART) technology to provide real-time detection of target DNA. The experimental results demonstrate the ability of the proposed device to discriminate among different concentrations of viral DNA with an excellent agreement with standard off-chip methods.

Purpose The aim of this study was to investigate gait kinematic parameters during stair ascent and descent after fibula free flap removal for facial reconstruction. Materials and Methods Eight patients who underwent facial reconstruction with fibula free flap removal ascended and descended 3 standard steps. Their movements were recorded by a motion analyzer; gait kinematic parameters were obtained and compared with those calculated in 8 control subjects. Results Stride time, percentage of swing, and support phases did not differ among healthy and operated limbs and control subjects (Kruskal-Wallis, P > .05). No significant differences were found for hip and knee movements, pelvis rotation and tilt, and body center of mass displacements. During stair descent, the patients had significantly larger pelvis inclinations than the control subjects (P Conclusions No functional limitations during stair performance were found. The only significant difference could indicate a minor control of the pelvis and should be used to define specific rehabilitative interventions.

This work reports on the design, simulation and fabrication of an autonomous microfluidic network. It is a part of a highly integrated, new analytical platform for the multiparametric detection of bio-organic molecules in extra-terrestrial environment. The proposed microfluidic system, made in SU-8 3050, allows to obtain an autonomous microfluidic network able to have simultaneous capillary filling and fresh solution into each site of detection avoiding cross-contamination among different sites. Computational Fluid Dynamics (CFD) simulations have been carried in order to verify the proper operation of the designed microfluidic network and to optimize it. Technological processes have been refined and adapted in order to ensure good adhesion, using low-temperature and low-pressure bonding avoiding the risk of breaking the glass slides. Experiments have been conducted to verify the autonomous capillary filling of the entire network and its rinsing with buffer solution. The experimental results are in good agreement with the simulations.

In this paper, we present an improved microfluidic network based on polydimethylsiloxane (PDMS) and thin film heaters for thermal treatment of biomolecules in lab-on-chip systems. It relies on the series connection of two thermally actuated valves, at both inlet and outlet of the network, in order to reduce leakage of sample when its process temperature approaches 100 °C. The spatial arrangement of valves and microfluidic channels in between has been optimized using COMSOL Multiphysics, through the investigation of the system thermal behavior. Taking into account the simulation results, the geometries of the heaters have been defined following standard microelectronic technologies and the microfluidic network has been fabricated by soft lithography. The experiments demonstrate that with the proposed configuration the liquid evaporation is strongly reduced since more than 80% of the sample is recovered after a practical thermal treatment experiment.

This work combines a lab-on-chip device with an electronic system for the achievement of a small-scale and low-cost thermal treatment of biomolecules. The lab-on-chip is a 1.2 mm-thick glass substrate hosting thin film resistor acting as heater and, on the other glass side, amorphous silicon diodes acting as temperature sensors. The electronic system controls the lab-on-chip temperature through a Proportional-Integral-Derivative algorithm. In particular, an electronic board infers the temperature measuring the voltage across the amorphous silicon diodes, which are biased with a constant forward current of 50 nA, and drives the heater to achieve the set-point temperature. The characterization of the whole system has been carried out implementing the thermal cycles necessary in the polymerase chain reaction technique for amplification of DNA. To this purpose, the lab-on-chip has been thermally coupled with another glass hosting a microfluidic network made in polydimethilsiloxane, and the time evolution of temperature has been carefully monitored. The measured performances in terms of heating rate, cooling rate and settling time demonstrate that the proposed system completely fulfills the requirements of the investigated biological application.

Physical Activity Effects in Elderly Female: Evaluation through Six-minute Walking Test Study aim. Aerobic capacity is very important for elderly. Daily living activities, such as walking, doing housework and shopping, require a good strength, but also a good aerobic endurance. The aim of this study was to verify the effects of regular training activity on a typical daily action, such as deambulation. Material and methods. Twenty-six volunteer elderly women randomly divided into two groups, group II (age = 64,5 ± 3,58 years, weight = 61,67 ± 4,25 kg, height = 1,62 ± 0,04 m; BMI = 23,67 ± 1,79 kg m-2), group I (age = 65,21 ± 4,59 years, weight = 63,36 ± 7,59 kg, height = 1,62 ± 0,06 m; BMI = 24,1 ± 1,98 kg m-2) participated in this study. Groups performed endurance training for eight weeks (2 days per week, 1 hour each, for experimental group II; 1 day per week, 1 hour each, for experimental group I). Exercises involved in many possibilities of physical expression to keeping all body in motion for at least 30 min. During the second 30 min exercises for abdominal and lower limb muscles were proposed. The physical efficacy was tested through 6 min walking test. Results. After the training all people increased the distance in the test. Group II participants significantly improved their distance (pre-training = 636.25 m, post-training = 673.75 m). Group I reported also improvements (pre = 568.21 m; post = 600 m), but with a minor level of significance. Conclusions. Specific regular physical activity provides significant benefits adopting two sessions per week but it is also important do not underestimate the possibility, where people can not, of a one training session per week. Regular and adequate levels of physical activity allow improving daily living activity helping the elderly to keep their autonomy as long as possible.

Purpose: Several studies analyzed muscle activity after pre-fatigue due to co-contraction, but the effects of antagonist contraction on agonist muscle performance are still incompletely known. The purpose is to determine if a previous workout of an antagonist muscle can affect muscular fatigue appearance during the subsequent agonist muscle performance. Methods: Surface EMG was used to estimate biceps brachii muscle fatigue during isometric contraction executed after previous contractions of the antagonist muscle. Eight expert male body-builders performed five series of isometric contractions (30 s) alternatively according to the agonist and antagonist protocols. Results: Within each repetition, for both biceps brachii muscles and experimental protocols, data showed a progressive fatigue, but no continuous quantitative decreasing trend of the median frequency of the spectrum along the five repetitions. Contraction time explained 1-91% (r 2 values) of the reduction of mean activation frequency along the 30 s (agonistic protocol); 0.7-92% for antagonistic protocol. The decrement in median frequency was only partially time- related. A repeated measures Anova found significant differences between subjects (p

Study design A study on active cervical range of motion (RoM) in healthy females of different ages. Objective Perform a three-dimensional quantitative analysis of active head-cervical RoM in healthy females to assess the relationship with age. Summary of background data Age-related variations in active cervical RoM are still partially unknown: some investigations demonstrated that age has no effect whatsoever on the primary movements, whereas other studies found an inverse proportionality between age and cervical RoM. Methods Three groups of females were compared: 22 aged 15 to 18 years (adolescents), 25 aged 20 to 30 years (young adults), and 16 aged 35 to 45 years (mid-aged women). Active flexion and extension, lateral bending and axial rotation were recorded via an optoelectronic system. After the mathematical exclusion of thoracic movements, cervical RoM was referred to head local reference system and calculated using the tilt/twist method. Data were compared using analysis of variance. Results Flexion and extension were larger in the adolescents (137 degrees ) than in the young adults (128 degrees ) and mid-aged women (127 degrees ). Lateral bending had similar ranges in the 3 groups: 103 degrees for adolescents, 101 degrees for young adults, and 100 degrees for mid-aged women. Axial rotation had similar ranges in the adolescents and in the mid-aged women (143 degrees ) and a slightly larger range in the young adults (151 degrees ). Primary movements were always associated with out-of-plane components. Conclusion In healthy females, between 15 and 45 years old, cervical RoMs in the principal planes decrease (except for rotation), but these variations are not statistically significant (P > 0.05).

To assess the relative contribution of rotation and translation of the temporomandibular condyle-disc assembly during opening and closing movements, free movements of maximum mouth opening and closing were recorded in healthy subjects (12 men, 14 women) using an optoelectronic three-dimensional motion analyzer. For each subject, the displacement of the lower interincisal point, the path of the condylar reference point, the degree of rotation around the three orthogonal rotational axes, and the relative contribution of translation and rotation were calculated during all movement of mouth opening and closing. The distance covered by the interincisor point and the rotational angle about the transverse axis at maximum mouth opening were larger in men than in women, but the difference cancelled after correcting for mandibular radius in the sagittal plane; mandibular rotation was always larger than translation, but never approaching 100%; opening and closing translations were similar within sex, but their paths were longer in men than in women (P < 0.05); rotational angles around vertical and sagittal axes were negligible; the linear correlation between maximum mandibular opening and condylar translation was minor and not significant. In normal subjects, mouth opening and closing as modeled at the interincisor point was determined more by mandibular rotation than by translation, but in no occasion a pure rotation was found. The percentage rotation was not identical during mouth opening and closing; female and male paths were not totally coincident; no correlation between maximum mandibular opening and condylar translation was found.

In this paper, we present a device that minimizes the effects of the temperature on light detection in lab-on-chip systems. The device is based on hydrogenated amorphous silicon p-type/intrinsic/n-type junction, fabricated on a glass substrate using thin-film technologies. The device structure is constituted by two series-connected amorphous silicon diodes: a blind one acting as dark reference and a photosensitive one. The signal measured at the output node of each element is equal to the difference of the current of the two diodes. This allows to minimize the temperature-dependent dark current contribution. The design of the photolithographic masks has been carefully carried out to pursue a perfect technological symmetry between the two diodes of the differential structure. Experimental data obtained by current-voltage characteristics show the correct operation of the individual diodes as well as the effectiveness of the differential structure to reject the common-mode signal induced by temperature variations. This feature makes the device a suitable candidate for analytical systems based on optical detection that involve thermal treatments.

Summary Objective: In humans, the opening movement of the mouth requires a complex combination of rotation in the lower temporomandibular joint compartment and of translation in the upper compartment. The aim of the current study was to quantitatively assess the percentage contribution of rotation and translation movements of the mandible at maximum mouth opening in normal, healthy individuals. Design: Free, habitual movements of mouth opening were recorded in 12 men and 15 women aged 19–30 years using an optoelectronic three-dimensional motion analyser. All subjects had a sound, complete, permanent dentitions with Angle Class I jaw relationships, without cast restorations or cuspal coverage, TMJ or craniocervical disorders. For each subject, the mandibular movements at the interincisor point (occlusal plane) were reconstructed, and, using suitable mathematical algorithms, divided into their rotation and gliding components. The relative contribution of the two components to the total movement was calculated for each frame of motion. In particular, the situation at maximum opening was assessed. Results: At maximum mouth opening, on average, men had significantly larger displacement of the mandibular interincisor point (56 mm versus 46 mm) and angle of rotation (34° versus 32°), than women. The percentage of mandibular movement explained by rotation at maximum mouth opening (77%) was not influenced by sex. The degree of rotation was significantly related to the displacement of the interincisor point: in women r 2 = 87%, in men, r 2 = 45%. Conclusions: Overall, in normal subjects with a healthy stomatognathic apparatus, mouth opening was more determined by mandibular rotation than by translation.

Sport performance during the execution of closed skills combines specific body and limb movements into codified patterns where stability and consistency may be more important than variability. Repeated sport movements can be investigated to assess the consistency of body trajectories. More consistent trajectories will result in more repeatable movements. The present study quantified the short-term consistency of body trajectories during the performance of the backward flic-flac, a technique of floor gymnastics. Nine experienced gymnasts (six men, three women), all of national level, performed 10 repetitions of backward flic-flac. An optoelectronic instrument was used for the detection of the three-dimensional movement of 13 body landmarks. The spatiotemporal consistency of repeated landmark trajectories was measured by the standard deviation between standardized trajectories. The results showed smaller standard deviation (larger consistency between landmark trajectories) in female than in male gymnasts. The analysis of the consistency of landmark trajectories could help gymnasts indicating which parts of the body do not repeat a selected movement with sufficient accuracy.

The goal of this study was to investigate the differences in physical fitness in relation to urban or rural living environment and between two countries: Italy and Croatia. Over 14,000 students were tested during physical education (PE) lessons. Croatian students were taller and heavier than Italian students, with women achieving better results in flexibility. Urban students were fitter in abdominal strength than rural students. Age seemed to be the major factor influencing the performance. This study's results indicate a trend: urban students performed “indoor” tests better than rural students; the opposite may be true for rural students. Living environment, socio-economic-status, or cultural correlation differences could be decreased during school experiences. Thus, PE programs could train all people toward an adequate, age-based fitness level.