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BACKGROUND: This multicenter study was performed with the intention to evaluate the exactness of the predicted CD34+ cell yield calculated by two leukapheresis programs of the cell separator COM.TEC upon the number of donor's circulating CD34+ cells and the blood volume processed. STUDY DESIGN AND METHODS: Patients and healthy donors (n = 166) received mobilization by chemotherapy and/or granulocyte colony-stimulating factor and underwent CD34+ cell harvest by the leukapheresis programs MNC or RV-PBSC (n = 203). RESULTS: CD34+ cells were collected by 112 harvests on MNC and by 91 collections on RV-PBSC. The median collection efficiency of CD34+ cells was significantly better for the program MNC than for RV-PBSC (p

Objectives The aim of this pilot study was to establish reference values for strong ion difference (SID) in umbilical cord blood and investigate the feasibility of evaluating fetal metabolism according to the comprehensive approach to acid–base abnormalities, based on Stewart's physiochemical theory. Study design A prospective observational study. Women who underwent an elective cesarean section at term ( n =40) were compared to women who completed a normal spontaneous delivery at term ( n =40). The primary outcome was the establishment of normal values for SID in the umbilical cord vein. We also compared acid–base variables in the umbilical vein between the groups. Results The apparent SID in the umbilical vein was 34.61±3.92mequiv./L after normal delivery and 35.98±2.56mequiv./L after elective cesarean section (the effective SID is 37.43±1.93 and 38.29±2.38mequiv./L, respectively). The pH values were similar in both groups, but the p CO 2 was significantly higher and the plasma principal weak acids (albumin and phosphate) were significantly lower after cesarean sections. Conclusions SID enables a comprehensive approach to acid–base abnormalities in the neonate, making it a potential additional tool for evaluating fetal acid–base status.