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Background Cervical cancer is one of the most frequent malignancies among females worldwide. Increasing evidence have indicated the participation of long noncoding RNAs (lncRNAs) in the progression and metastasis of cervical cancer. Our present study was conducted to explore the effects of lncRNA plasmacytoma variant translocation 1 (PVT1) on the progression of cervical cancer and the underlying mechanisms. Materials and methods Expressions of PVT1, miR-140-5p and Smad3 in cervical cancer cell lines were detected by qRT-PCR and western blotting. Bioinformatics analysis and luciferase assays were used to elucidate the potential correlations between PVT1, miR-140-5p and Smad3. The roles of PVT1 on the progression of cervical cancer cells were determined by transfecting sh-RNA through series function assays such as colony formation assay, wound healing assay, transwell assay. Results PVT1 and Smad3 were upregulated, and miR-140-5p was downregulated in cervical cancer cells. PVT1 could bind directly with miR-140-5p, and Smad3 was a downstream target of miR-140-5p. Inhibition of PVT1 could enhance expression of miR-140-5p, inhibit the expression of Smad3, significantly inhibited the proliferation, migration, invasion in cervical cancer cells. While transfection of miR-140-5p inhibitor could partially reverse the above changes in cervical cancer cells. Conclusions The results revealed that PVT1 could promote the proliferation and metastasis via increasing the Smad3 expression by sponging miR-140-5p, which might be a promising prognostic and therapeutic target for cervical cancer.

This paper shows a method to calibrating the non-uniformity of the x-ray backscatter image in a side-beam x-ray detection system. The flying spot of x-ray beam cross the luggage under detection per cycle, and every point of the detector being irradiated by flying spot can be taken as an assumed detector. Using calibrators of different thickness and combined with the corresponding mathematical model, the greyscale of the image is uniformed and calibration is achieved.

Topology optimization is a kind of optimized method, which can seek for an optimal allocation of structural material according to the load,constraints and optimization goals .To achieve the lightweight design of the top beam, establishing a minimum strain as the objective function, the volume fraction as the constraint conditions, and the density of design domain as the design variables,implementing the topology optimization for the top beam,the optimal distribution of top beam material is obtained, which provide a basis for the manufacture of top beam.

This paper elaborates on defects in the Petrol- China Changqing Oilfield Cooperation Management Pro- ject in an attempt to improve future cooperative efforts. The paper focuses on the drawbacks in the existing manage- ment process and analyzes the process by using current information management methods. This effort is made in hopes of arriving at the most efficient and effective method to redesign the existing processes.