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Made available in DSpace on 2018-12-11T17:21:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01. Added 1 bitstream(s) on 2019-10-09T18:31:56Z : No. of bitstreams: 1 S2359-39972018000300366.pdf: 123932 bytes, checksum: 884f7b3ea5771ca0dc08ec15ac8f5036 (MD5) Made available in DSpace on 2018-12-11T17:21:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01. Added 1 bitstream(s) on 2019-10-09T18:31:56Z : No. of bitstreams: 1 S2359-39972018000300366.pdf: 123932 bytes, checksum: 884f7b3ea5771ca0dc08ec15ac8f5036 (MD5) Made available in DSpace on 2018-12-11T17:21:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01. Added 1 bitstream(s) on 2019-10-09T18:31:56Z : No. of bitstreams: 1 S2359-39972018000300366.pdf: 123932 bytes, checksum: 884f7b3ea5771ca0dc08ec15ac8f5036 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: Graves’ ophthalmopathy (GO) is an autoimmune disease that leads to ocular proptosis caused by fat accumulation and inflammation, and the main treatment is corticosteroid therapy. Retinoid acid receptor-alpha (RARα) seems to be associated with inflammation and adipocyte differentiation. This study aimed to assess the effect of glucocorticoid treatment on orbital fibroblasts of GO patient treated or not with different glucocorticoid doses. Materials and methods: Orbital fibroblasts collected during orbital decompression of a female patient with moderately severe/severe GO were cultivated and treated with 10 nM and 100 nM dexamethasone (Dex). RARα gene expression in the treated and untreated cells was then compared. Results: Fibroblast RARα expression was not affected by 100 nM Dex. On the other hand, RARα expression was 24% lower in cells treated with 10 nM Dex (p < 0.05). Conclusions: Orbital fibroblasts from a GO patient expressed the RARα gene, which was unaffected by higher, but decreased with lower doses of glucocorticoid. Divisão de Endocrinologia Departamento de Medicina Interna Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp) Instituto Federal de Educação Ciência e Tecnologia do Estado de São Paulo (IFSP) Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeça e Pescoço Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp) Divisão de Endocrinologia Departamento de Medicina Interna Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp) Departamento de Oftalmologia Otorrinolaringologia e Cirurgia de Cabeça e Pescoço Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo (Unesp) FAPESP: #:03/03651-0 FAPESP: 2011/18464-8

The purpose of this study it was to evaluate the frequency of Multiple Endocrine Neoplasia type 1 (MEN1) in patients with pituitary adenoma and to perform genetic analysis and familial screening of those individuals afflicted with MEN1. 144 patients with pituitary adenoma at Botucatu Medical School, UNESP—Univ Estadual Paulista, were assessed retrospectively for MEN1 during the years of 2005–2011. The patients were evaluated for the presence of primary hyperparathyroidism (PHP) and enteropancreatic tumors. Genetic analysis was performed for the individuals with clinically diagnosed MEN1. Thirteen patients met the diagnostic criteria for MEN1, but three individuals belong to the same family and they were considered as a single MEN1 event, revealing 7.7 % frequency of MEN1 in this patient group. Genetic analysis showed MEN1 mutations in four index cases: IVS4+1 G>A, IVS3-6 C>T, c.1547insC and a new D180A mutation. One patient did not agree to participate in the genetic study and another one was referred for follow up in other hospital. Only polymorphisms were found in the other individuals, one of which was novel. We identified a high frequency of MEN1 in pituitary adenoma patients. Since PHP is one of the most common MEN1 tumor and patients are mostly asymptomatic, we suggest that all pituitary adenoma patients have their calcium profile analyzed.

Made available in DSpace on 2018-12-11T17:29:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-08-01. Added 1 bitstream(s) on 2019-10-09T18:32:39Z : No. of bitstreams: 1 S1807-59322016000800470.pdf: 341861 bytes, checksum: 531786629f0ab751fa3f3a1b78516fec (MD5) OBJECTIVE: The aim of this study was to evaluate the roles of the Taql and Bsml vitamin D receptor gene polymorphisms in hospital mortality of burn patients. METHODS: In total, 105 consecutive burn injury patients over 18 years in age who were admitted to the Burn Unit of Bauru State Hospital from January to December 2013 were prospectively evaluated. Upon admission, patient demographic information was recorded and a blood sample was taken for biochemical analysis to identify the presence of the Taql(rs731236) and Bsml(rs1544410) polymorphisms. All of the patients were followed over their hospital stay and mortality was recorded. RESULTS: Eighteen of the patients did not sign the informed consent form, and there were technical problems with genotype analysis for 7 of the patients. Thus, 80 patients (mean age, 42.5�16.1 years) were included in the final analysis. In total, 60% of the patients were male, and 16.3% died during the hospital stay. The genotype frequencies for the Taql polymorphism were 51.25% TT, 41.25% TC and 7.50% CC; for the Bsml polymorphism, they were 51.25% GG, 42.50% GA and 6.25% AA. In logistic regression analysis, after adjustments for age, gender and total body surface burn area, there were no associations between the Taql (OR: 1.575; CI95%: 0.148- 16.745; p=0.706) or Bsml (OR: 1.309; CI95%: 0.128-13.430; p=0.821) polymorphisms and mortality for the burn patients. CONCLUSIONS: Our results suggest that the Taql and Bsml vitamin D receptor gene polymorphisms are not associated with hospital mortality of burn patients. Universidade Estadual Paulista – UNESP Faculdade de Medicina de Botucatu Departamento de Medicina Interna Instituto Federal S�o Paulo – IFSP, Campus S�o Roque Universidade Estadual Paulista – UNESP Faculdade de Medicina de Botucatu Departamento de Medicina Interna

OBJETIVO: Verificar o perfil dos hormônios tireóideos (HTs) em pacientes pós-menopausa portadoras de carcinoma de mama (CaM). SUJEITOS E MÉTODOS: Participaram 12 pacientes com CaM em estádio I ou II sem intervenções que pudessem interferir na progressão tumoral e um grupo controle com 18 pacientes em pós-menopausa sem CaM. Foram dosados os níveis séricos de anticorpo antitiroperoxidase (TPOAB), hormônio estimulante da tireoide (TSH), tiroxina livre (T4L), estradiol (E2), hormônio folículo estimulante (FSH) e hormônio luteinizante (LH) antes e após a cirurgia, e realizada a imunoistoquímica dos receptores de estrógeno (ER) e progesterona (PR). RESULTADOS: Quatro pacientes com CaM apresentaram alterações do perfil hormonal tireoidiano: dois hipertireoidismo, um hipotireoidismo e positividade TPO-AB, todas com ER e PR positivos. Os níveis de TSH dessas pacientes não foram diferentes dos níveis encontrados no grupo controle (1,89 ± 1,56 vs. 2,86 ± 3,12 mUI/mL), porém os níveis de T4L nas pacientes com CaM foram estatisticamente maiores que o controle (1,83 ± 0,57 vs. 1,10 ± 0,20 ng/dL). CONCLUSÃO: Esses resultados reforçam a necessidade de avaliação do status tireoidiano em pacientes com CaM, uma vez que, na ausência de E2, mudanças clínicas nos HTs podem atuar em vias controladas pelo E2. OBJECTIVE: The aim of this study was to determine thyroid hormone (TH) profile in postmenopausal patients with breast cancer (BC). SUBJECTS AND METHODS: 12 CaM patients stages I or II, without interventions that could interfere with tumor progression were selected, as well as and a control group with 18 postmenopausal women without CaM. We measured serum anti-thyroperoxidase antibody (TPOAB), thyroid-stimulating hormone (TSH), free thyroxine (T4L), estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH), before and after surgery, besides immunohistochemistry for estrogen (ER) and progesterone (PR) receptors. RESULTS: Four patients with CaM showed changes in thyroid hormone profile: two had hyperthyroidism, one hypothyroidism, and one was positive for TPO-AB. All of them positive for ER and PR. TSH levels in breast cancer patients were not different from levels found in the control group (1.89 ± 1.56 vs. 2.86 ± 3.12 mIU/mL), but the levels of T4L in patients with CaM were statistically higher than those of the control group (1.83 ± 0.57 vs. 1.10 ± 0.20 ng/dL). CONCLUSION: These results reinforce the need for assessment of thyroid status in CaM patients, since in the absence of E2, changes in clinical HTs can act in E2-controlled processes.

Made available in DSpace on 2015-02-02T12:39:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-10-01Bitstream added on 2015-02-02T13:07:53Z : No. of bitstreams: 1 S0004-27492014000500339.pdf: 579808 bytes, checksum: e8fd687573ee4954ed41efeed3136c7f (MD5) Made available in DSpace on 2015-02-02T12:39:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-10-01Bitstream added on 2015-02-02T13:07:53Z : No. of bitstreams: 1 S0004-27492014000500339.pdf: 579808 bytes, checksum: e8fd687573ee4954ed41efeed3136c7f (MD5) Made available in DSpace on 2015-02-02T12:39:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-10-01Bitstream added on 2015-02-02T13:07:53Z : No. of bitstreams: 1 S0004-27492014000500339.pdf: 579808 bytes, checksum: e8fd687573ee4954ed41efeed3136c7f (MD5) Made available in DSpace on 2015-02-02T12:39:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-10-01Bitstream added on 2015-02-02T13:07:53Z : No. of bitstreams: 1 S0004-27492014000500339.pdf: 579808 bytes, checksum: e8fd687573ee4954ed41efeed3136c7f (MD5) Made available in DSpace on 2015-02-02T12:39:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-10-01Bitstream added on 2015-02-02T13:07:53Z : No. of bitstreams: 1 S0004-27492014000500339.pdf: 579808 bytes, checksum: e8fd687573ee4954ed41efeed3136c7f (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Universidade Estadual Paulista Botucatu Medical School Internal Medicine Department Universidade Estadual Paulista Botucatu Medical School Ophthalmology, Otorhinolaryngology and Head and Neck Department Universidade Estadual Paulista Botucatu Medical School Internal Medicine Department Universidade Estadual Paulista Botucatu Medical School Ophthalmology, Otorhinolaryngology and Head and Neck Department

Made available in DSpace on 2015-08-26T19:19:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-06-01. Added 1 bitstream(s) on 2015-08-31T13:05:04Z : No. of bitstreams: 1 S2359-39972015000300273.pdf: 130120 bytes, checksum: 6dc2ae5dfe61f973ea9aecc7248da395 (MD5) Made available in DSpace on 2015-08-26T19:19:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-06-01. Added 1 bitstream(s) on 2015-08-31T13:05:04Z : No. of bitstreams: 1 S2359-39972015000300273.pdf: 130120 bytes, checksum: 6dc2ae5dfe61f973ea9aecc7248da395 (MD5) Made available in DSpace on 2015-08-26T19:19:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-06-01. Added 1 bitstream(s) on 2015-08-31T13:05:04Z : No. of bitstreams: 1 S2359-39972015000300273.pdf: 130120 bytes, checksum: 6dc2ae5dfe61f973ea9aecc7248da395 (MD5) Made available in DSpace on 2015-08-26T19:19:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-06-01. Added 1 bitstream(s) on 2015-08-31T13:05:04Z : No. of bitstreams: 1 S2359-39972015000300273.pdf: 130120 bytes, checksum: 6dc2ae5dfe61f973ea9aecc7248da395 (MD5) Made available in DSpace on 2015-08-26T19:19:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-06-01. Added 1 bitstream(s) on 2015-08-31T13:05:04Z : No. of bitstreams: 1 S2359-39972015000300273.pdf: 130120 bytes, checksum: 6dc2ae5dfe61f973ea9aecc7248da395 (MD5) Graves’ ophthalmopathy (GO) is one of the most severe clinical manifestations of Graves’ disease (GD), and its treatment might involve high-dose glucocorticoid therapy. The higher incidence of GO among females, and the reported association between polymorphisms of estrogen receptor (ER) and GD susceptibility have led us to question the role of estrogen and its receptor in GO pathogenesis. We, thus, assessed estrogen receptor-alpha (ERA) gene expression in cultures of orbital fibroblasts from a patient with GO before (controls) and after treatment with 10 nM and 100 nM dexamethasone (DEX). Orbital fibroblasts showed ERA gene expression. In the cells treated with 10 nM and 100 nM DEX, ERA gene expression was, respectively, 85% higher and 74% lower, than in the control group. We concluded that ERA gene expression is found in the orbital fibroblasts of patient with GO, which may be affected by glucocorticoids in a dose-related manner. Arch Endocrinol Metab. 2015;59(3):273-6 Sao Paulo State University Botucatu Medical School Internal Medicine Department Unesp Sao Paulo State University Botucatu Medical School Internal Medicine Department Unesp

The thyroid hormones (THs), triiodothyronine (T3) and thyroxine (T4), are essential for survival; they are involved in the processes of development, growth, and metabolism. In addition to hyperthyroidism or hypothyroidism, THs are involved in other diseases. The role of THs in the development and differentiation of mammary epithelium is well established; however, their specific role in the pathogenesis of breast cancer (BC) is controversial. Steroid hormones affect many human cancers and the abnormal responsiveness of the mammary epithelial cells to estradiol (E2) in particular is known to be an important cause for the development and progression of BC. The proliferative effect of T3 has been demonstrated in various types of cancer. In BC cell lines, T3 may foster the conditions for tumor proliferation and increase the effect of cell proliferation by E2; thus, T3 may play a role in the development and progression of BC. Studies show that T3 has effects similar to E2 in BC cell lines. Despite controversy regarding the relationship between thyroid disturbances and the incidence of BC, studies show that thyroid status may influence the development of tumor, proliferation and metastasis.

Made available in DSpace on 2015-12-07T15:30:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2014. Added 1 bitstream(s) on 2015-12-07T15:52:57Z : No. of bitstreams: 1 PMC3950583.pdf: 2375661 bytes, checksum: fa0182a0b8ea0d24d60647be62a0a9d1 (MD5) We investigated thyroid hormone levels in menopausal BrC patients and verified the action of triiodothyronine on genes regulated by estrogen and by triiodothyronine itself in BrC tissues. We selected 15 postmenopausal BrC patients and a control group of 18 postmenopausal women without BrC. We measured serum TPO-AB, TSH, FT4, and estradiol, before and after surgery, and used immunohistochemistry to examine estrogen and progesterone receptors. BrC primary tissue cultures received the following treatments: ethanol, triiodothyronine, triiodothyronine plus 4-hydroxytamoxifen, 4-hydroxytamoxifen, estrogen, or estrogen plus 4-hydroxytamoxifen. Genes regulated by estrogen (TGFA, TGFB1, and PGR) and by triiodothyronine (TNFRSF9, BMP-6, and THRA) in vitro were evaluated. TSH levels in BrC patients did not differ from those of the control group (1.34 ± 0.60 versus 2.41 ± 1.10 μ U/mL), but FT4 levels of BrC patients were statistically higher than controls (1.78 ± 0.20 versus 0.95 ± 0.16 ng/dL). TGFA was upregulated and downregulated after estrogen and triiodothyronine treatment, respectively. Triiodothyronine increased PGR expression; however 4-hydroxytamoxifen did not block triiodothyronine action on PGR expression. 4-Hydroxytamoxifen, alone or associated with triiodothyronine, modulated gene expression of TNFRSF9, BMP-6, and THRA, similar to triiodothyronine treatment. Thus, our work highlights the importance of thyroid hormone status evaluation and its ability to interfere with estrogen target gene expression in BrC samples in menopausal women. Department of Biological Science, São Paulo Federal Institute (IFSP), 18136-540 São Roque, SP, Brazil ; Department of Internal Medicine, Division of Endocrinology and Metabolism, UNESP, 18618-000 Botucatu, SP, Brazil. Department of Internal Medicine, Division of Endocrinology and Metabolism, UNESP, 18618-000 Botucatu, SP, Brazil. Department of Biological Science, São Paulo Federal Institute (IFSP), 18136-540 São Roque, SP, Brazil ; Department of Internal Medicine, Division of Endocrinology and Metabolism, UNESP, 18618-000 Botucatu, SP, Brazil. Department of Internal Medicine, Division of Endocrinology and Metabolism, UNESP, 18618-000 Botucatu, SP, Brazil.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:03:19Z No. of bitstreams: 1 S0004-27302012000800018.pdf: 138617 bytes, checksum: 6272e3edefd445c844169dd6dec96675 (MD5) Made available in DSpace on 2013-08-22T19:03:19Z (GMT). No. of bitstreams: 1 S0004-27302012000800018.pdf: 138617 bytes, checksum: 6272e3edefd445c844169dd6dec96675 (MD5) Previous issue date: 2012-11-01 Made available in DSpace on 2013-09-30T20:05:05Z (GMT). No. of bitstreams: 2 S0004-27302012000800018.pdf: 138617 bytes, checksum: 6272e3edefd445c844169dd6dec96675 (MD5) S0004-27302012000800018.pdf.txt: 14858 bytes, checksum: f0c885a3c1b012752d54dfd77f638c28 (MD5) Previous issue date: 2012-11-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:16:41Z No. of bitstreams: 2 S0004-27302012000800018.pdf: 138617 bytes, checksum: 6272e3edefd445c844169dd6dec96675 (MD5) S0004-27302012000800018.pdf.txt: 14858 bytes, checksum: f0c885a3c1b012752d54dfd77f638c28 (MD5) Made available in DSpace on 2014-05-20T15:16:41Z (GMT). No. of bitstreams: 2 S0004-27302012000800018.pdf: 138617 bytes, checksum: 6272e3edefd445c844169dd6dec96675 (MD5) S0004-27302012000800018.pdf.txt: 14858 bytes, checksum: f0c885a3c1b012752d54dfd77f638c28 (MD5) Previous issue date: 2012-11-01 A osteomalacia oncogênica é um diagnóstico clínico desafiador, caracterizado pela perda renal de fosfato e baixos níveis de 1,25-di-hidroxivitamina D3, ocorrendo na presença de um tumor produtor de altos níveis de fator de crescimento de fibroblasto 23. No entanto, é possível que se trate muito mais de uma falha de diagnóstico clínico do que propriamente uma doença rara. Os autores relatam o caso de um homem de 42 anos com histórico de fraqueza muscular progressiva por cinco anos e restrição à cadeira de rodas, sem diagnóstico. Seus exames laboratoriais evidenciavam baixos níveis de fósforo. A remoção cirúrgica de um hemangiopericitoma detectado previamente em cavidade nasal levou à resolução completa dos sintomas. Os autores enfatizam que, mesmo com a etiologia já evidenciada, o paciente consultou diversos clínicos no decorrer dos cinco anos até que fossem instituídos o diagnóstico e o tratamento adequados. Arq Bras Endocrinol Metab. 2012;56(8):570-3 Diagnosing oncogenic osteomalacia is still a challenge. The disorder is characterized by osteomalacia caused by renal phosphate wasting and low serum concentration of 1,25-dihydroxyvitamin D3 occurring in the presence of a tumor that produces high levels of fibroblast growth factor 23. However, it is possible that the disease is much more misdiagnosed than rare. We present the case of a 42-year-old man with a long-term history of undiagnosed progressive muscle weakness. His laboratory results mainly showed low serum phosphate. Surgical removal of a nasal hemangiopericytoma that had been diagnosed five years earlier, brought him to a symptom-free condition. Even though knowing the underlying etiology would explain his osteomalacia, the patient sought medical help from countless physicians for five consecutive years, and only after adequate treatment a rewarding outcome was achieved. Arq Bras Endocrinol Metab. 2012;56(8):570-3 Universidade Estadual de São Paulo Faculdade de Medicina de Botucatu Department of Medicine Universidade Federal de São Paulo (UNIFESP) Department of Neurology and Neurosurgery Metabolism and Molecular Medicine Northwestern University Division of Endocrinology

There are several breast cancer experimental models including cell lines, which are commonly used due to ease of handling and storage. However, the continued propagation of cell lines and distribution among laboratories results in genetic drift and distancing from the in-vivo model. Primary organ culture of breast cancer slices may produce biological responses with high standard deviation for different samples, reflecting the heterogeneity of different tumors. Thus, the organ culture model system offers a new perspective to the results obtained in the cell lines and offers an alternative for studies that seek to individualize treatment for each patient, an increasingly prominent concern in current cancer therapy.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:41:21Z No. of bitstreams: 1 S0066-782X2011000900008.pdf: 317389 bytes, checksum: 050fa42a7102f7f1c3eac2678f8a8138 (MD5) Made available in DSpace on 2013-08-22T18:41:21Z (GMT). No. of bitstreams: 1 S0066-782X2011000900008.pdf: 317389 bytes, checksum: 050fa42a7102f7f1c3eac2678f8a8138 (MD5) Previous issue date: 2011-07-01 Made available in DSpace on 2013-09-30T19:27:50Z (GMT). No. of bitstreams: 2 S0066-782X2011000900008.pdf: 317389 bytes, checksum: 050fa42a7102f7f1c3eac2678f8a8138 (MD5) S0066-782X2011000900008.pdf.txt: 43869 bytes, checksum: 776bf0fb95907daa85e4d92e05ccaa4f (MD5) Previous issue date: 2011-07-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:07:41Z No. of bitstreams: 2 S0066-782X2011000900008.pdf: 317389 bytes, checksum: 050fa42a7102f7f1c3eac2678f8a8138 (MD5) S0066-782X2011000900008.pdf.txt: 43869 bytes, checksum: 776bf0fb95907daa85e4d92e05ccaa4f (MD5) Made available in DSpace on 2014-05-20T15:07:41Z (GMT). No. of bitstreams: 2 S0066-782X2011000900008.pdf: 317389 bytes, checksum: 050fa42a7102f7f1c3eac2678f8a8138 (MD5) S0066-782X2011000900008.pdf.txt: 43869 bytes, checksum: 776bf0fb95907daa85e4d92e05ccaa4f (MD5) Previous issue date: 2011-07-01 FUNDAMENTO: Treinamento físico (TF) aumenta a sensibilidade dos hormônios tireoidianos (HT) e a expressão gênica de estruturas moleculares envolvidas no movimento intracelular de cálcio do miocárdio, enquanto a restrição alimentar (RIA) promove efeitos contrários ao TF. OBJETIVO: Avaliar os efeitos da associação TF e RIA sobre os níveis plasmáticos dos HT e a produção de mRNA dos receptores HT e estruturas moleculares do movimento de cálcio do miocárdio de ratos. MÉTODOS: Utilizaram-se ratos Wistar Kyoto divididos em: controle (C, n = 7), RIA (R50, n = 7), exercício físico (EX, n = 7) e exercício físico + RIA (EX50, n = 7). A RIA foi de 50% e o TF foi natação (1 hora/dia, cinco sessões/semana, 12 semanas consecutivas). Avaliaram-se as concentrações séricas de triiodotironina (T3), tiroxina (T4) e hormônio tireotrófico (TSH). O mRNA da bomba de cálcio do retículo sarcoplasmático (SERCA2a), fosfolamban (PLB), trocador Na+/Ca+2 (NCX), canal lento de cálcio (canal-L), rianodina (RYR), calsequestrina (CQS) e receptor de HT (TRα1 e TRβ1) do miocárdio foram avaliados por reação em cadeia da polimerase (PCR) em tempo real. RESULTADOS: RIA reduziu o T4, TSH e mRNA do TRα1 e aumentou a expressão da PLB, NCX e canal-L. TF aumentou a expressão do TRβ1, canal-L e NCX. A associação TF e RIA reduziu T4 e TSH e aumentou o mRNA do TRβ1, SERCA2a, NCX, PLB e correlação do TRβ1 com a CQS e NCX. CONCLUSÃO: Associação TF e RIA aumentou o mRNA das estruturas moleculares cálcio transiente, porém o eixo HT-receptor não parece participar da transcrição gênica dessas estruturas. BACKGROUND: Chronic exercise and food restriction (FR) have directionally opposite changes in transcription of molecular structures of calcium handling and thyroid hormone (TH) status. OBJECTIVE: Evaluate the association of chronic exercise and FR on serum thyroid hormones and gene transcription of molecular structures of intracellular calcium transients and thyroid receptors in myocardium of rats. METHODS: Male Wistar Kyoto rats, divided into two groups: control (C, n = 7), FR (R50, n = 7), chronic exercise (EX, n = 7) and chronic exercise + FR (EX50, n = 7). FR was of 50% and exercise was swimming (1 hour/day, 5 days/week, during 12 weeks). Serum concentrations of T3, T4 and TSH were determined. The mRNA gene expression of the sarcoplasmatic reticulum calcium pump (SERCA2a), phospholamban (PLB), Na+/Ca+2 exchanger (NCX), calcium channel L-type (L-channel), ryanodine (RYR), calsequestrin (CQS) and HT receptor (TRα1 and TRβ1) of the myocardium was performed by PCR real-time. RESULTS: FR reduced serum levels of T4 and TSH and TRα1 mRNA and increased the expression of PLB, NCX and L-channel. Exercise increased the TRβ1 receptor, L-channel and NCX. The association of exercise and FR reduced plasma T4 and TSH, TRβ1 mRNA increase, SERCA2a, NCX and PLB, and there was a significant correlation of TRβ1 with CQS and NXC. CONCLUSION: Chronic exercise and food restriction increased the mRNA of transient Ca2+ proteins; however, TH-receptor axis cannot participate in the transcription of mRNA of myocardial calcium transient proteins. FUNDAMENTO: Entrenamiento físico (EF) aumenta la sensibilidad de las hormonas tiroideas (HT) y la expresión génica de estructuras moleculares envueltas en el movimiento intracelular de calcio del miocardio, mientras que la restricción alimenticia (RA) promueve efectos contrarios al EF. OBJETIVO: Evaluar los efectos de la asociación EF y RA sobre los niveles plasmáticos de los HT y la producción de ARNm de los receptores HT y estructuras moleculares del movimiento de calcio del miocardio de ratones. MÉTODOS: Se utilizaron ratones Wistar Kyoto divididos en: control (C, n = 7), RA (R50, n = 7), ejercicio físico (EX, n = 7) y ejercicio físico + RA (EX50, n = 7). La RA fue de 50% y el EF fue natación (1 hora/día, cinco sesiones/semana, 12 semanas consecutivas). Se evaluaron las concentraciones séricas de triyodotironina (T3), tiroxina (T4) y hormona tireotrófico (TSH). El ARNm de la bomba de calcio del retículo sarcoplasmático (SERCA2a), fosfolamban (PLB), intercambiador Na+/Ca+2 (NCX), canal lento de calcio (canal-L), rianodina (RYR), calsequestrina (CQS) y receptor de HT (TRα1 y TRβ1) del miocardio fueron evaluados por reacción en cadena de la polimerasa (PCR) en tiempo real. RESULTADOS: RA redujo el T4, TSH y ARNm del TRα1 y aumentó la expresión de la PLB, NCX y canal-L. EF aumentó la expresión del TRβ1, canal-L y NCX. La asociación EF y RA redujo T4 y TSH y aumentó el ARNm del TRβ1, SERCA2a, NCX, PLB y correlación del TRβ1 con la CQS y NCX. CONCLUSIÓN: Asociación EF y RA aumentó el ARNm de las estructuras moleculares calcio transiente, sin embargo el eje HT-receptor no parece participar de la transcripción génica de esas estructuras. Universidade Federal de Mato Grosso Instituto de Ciências da Saúde Universidade Estadual Paulista Faculdade de Medicina Universidade Estadual Paulista Faculdade de Medicina

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:59:49Z No. of bitstreams: 1 S0004-27302007000900008.pdf: 96437 bytes, checksum: 5c4bdd1d5d17b3cd7e07afe6bdf9f76e (MD5) Made available in DSpace on 2013-08-22T18:59:49Z (GMT). No. of bitstreams: 1 S0004-27302007000900008.pdf: 96437 bytes, checksum: 5c4bdd1d5d17b3cd7e07afe6bdf9f76e (MD5) Previous issue date: 2007-12-01 Made available in DSpace on 2013-09-30T19:57:11Z (GMT). No. of bitstreams: 2 S0004-27302007000900008.pdf: 96437 bytes, checksum: 5c4bdd1d5d17b3cd7e07afe6bdf9f76e (MD5) S0004-27302007000900008.pdf.txt: 19752 bytes, checksum: 6789083165b81218f2b3db6d2e17f82b (MD5) Previous issue date: 2007-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:14:51Z No. of bitstreams: 2 S0004-27302007000900008.pdf: 96437 bytes, checksum: 5c4bdd1d5d17b3cd7e07afe6bdf9f76e (MD5) S0004-27302007000900008.pdf.txt: 19752 bytes, checksum: 6789083165b81218f2b3db6d2e17f82b (MD5) Made available in DSpace on 2014-05-20T15:14:51Z (GMT). No. of bitstreams: 2 S0004-27302007000900008.pdf: 96437 bytes, checksum: 5c4bdd1d5d17b3cd7e07afe6bdf9f76e (MD5) S0004-27302007000900008.pdf.txt: 19752 bytes, checksum: 6789083165b81218f2b3db6d2e17f82b (MD5) Previous issue date: 2007-12-01 Dois pacientes índices da família analisada neste estudo foram submetidos a adrenalectomia bilateral devido a feocromocitoma. Foi, então, realizado o estudo genético dos pacientes e de sete parentes de primeiro grau. Os dois pacientes com feocromocitoma e dois outros membros assintomáticos da família apresentaram a mutação c496G>T no exon 3 do gene VHL. A família perdeu seguimento médico. Três anos após a realização da avaliação genética, a irmã dos pacientes, portadora da mutação, foi encaminhada para o nosso serviço após uma gestação complicada por pré-eclampsia. Ela referia paroxismos sugestivos de feocromocitoma, mas as metanefrinas urinárias eram negativas. Entretanto, a tomografia computadorizada de abdômen evidenciou uma massa adrenal que também se contrastou na cintilografia com metaiodobenzilguanidina (MIBG). Esse estudo mostra que a análise molecular do paciente índice pode levar à identificação de parentes assintomáticos portadores da mutação. Além disso, mesmo com as metanefrinas urinárias negativas, a identificação de uma mutação específica levou a um aumento da suspeita e detecção de feocromocitoma na irmã dos afetados pela doença. The two index patients of the family analyzed in this study had undergone bilateral adrenalectomy for pheochromocytomas. This prompted genetic analyses of the probands and seven first-degree relatives. The two pheochromocytoma patients and two additional asymptomatic family members were found to harbor a mutation c496G>T in exon 3 of the VHL gene. The family was then lost to systematic follow-up. Three years after performing the initial genetic evaluation, the sister of the probands, who was known to carry the same VHL germline mutation, was referred to our service after a pregnancy that was complicated by preeclampsia. She reported paroxysms suggestive for pheochromocytoma, but her urinary metanephrines were negative. However, computerized tomography of the abdomen showed an adrenal mass that was also positive on metaiodobenzylguanidine (MIBG) scintigraphy. This study illustrates that molecular analysis of the index patient(s) can lead to the identification of presymptomatic relatives carrying the mutation. Moreover, despite negative urinary metanephrines, the identification of a specific mutation has led to an increased suspicion and detection of a pheochromocytoma in the sister of the probands. Universidade Estadual Paulista Faculdade de Medicina Departamento de Clínica Médica Northwestern University Division of Endocrinology, Metabolism, and Molecular Medicine Universidade Estadual Paulista Faculdade de Medicina Departamento de Clínica Médica

Univ Sao Paulo State, UNESP, Dept Clin Med, Botucatu Sch Med, BR-18618000 Botucatu, SP, Brazil

Our last research showed that association of physical training (PT) and food restriction (FR) increases inotropic myocardial contractile response in rats.PURPOSE: To test the hypothesis that myocardial contractile response improvement in PT and FR rats is related to increase in the intracellular cal