1. DACH1, a novel target of miR-218, participates in the regulation of cell viability, apoptosis, inflammatory response, and epithelial-mesenchymal transition process in renal tubule cells treated by high-glucose
- Author
-
Shou-Bao Wang, Jie-Min Wang, Jing Liu, Ying-Li Zhang, Hong Yin, and Cai-Ling He
- Subjects
Epithelial-Mesenchymal Transition ,Cell Survival ,Dachshund ,proliferation ,030232 urology & nephrology ,Inflammation ,Apoptosis ,030204 cardiovascular system & hematology ,Critical Care and Intensive Care Medicine ,Kidney ,lcsh:RC870-923 ,Cell Line ,03 medical and health sciences ,0302 clinical medicine ,Laboratory Study ,Medicine ,Humans ,Viability assay ,Epithelial–mesenchymal transition ,microrna-218 ,Eye Proteins ,Transcription factor ,Renal tubule ,business.industry ,General Medicine ,lcsh:Diseases of the genitourinary system. Urology ,eye diseases ,diabetic kidney disease ,MicroRNAs ,Glucose ,Nephrology ,inflammation ,High glucose ,Cancer research ,dach1 ,medicine.symptom ,business ,Transcription Factors - Abstract
Objective: This report was designed to assess the functional role of miR-218/dachshund family transcription factor 1 (DACH1) in diabetic kidney disease (DKD) and investigate its possible molecular mechanism. Materials and Methods: From the GEO database, we downloaded different datasets for analyzing the expression of miR-218 and DACH1 in DKD. TargetScan was adopted to predict the binding sites between miR-218 and DACH1, which was further verified by dual-luciferase reporter assays. The renal proximal tubule cells (HK-2) treated with high glucose (HG) were used as an in vitro model. QRT-PCR and western blot were used to determine the expression of DACH1 and other relative factors. Cell counting kit-8 and flow cytometer were applied to detect cell viability and apoptosis. The levels of inflammatory cytokines were determined by an ELISA assay. Results: A prominent raise of miR-218 was observed in DKD through bioinformatics analysis, which was further confirmed in the HG-induced model. DACH1 is a target of miR-218. miR-218 reduced cell viability and induced apoptosis by negatively regulating DACH1. Moreover, upregulating miR-218 in HG models increased the concentrations of pro-inflammatory cytokines TNF-α and IL-1β, reduced the level of anti-inflammatory cytokine IL-10, and promoted the epithelial-mesenchymal transition (EMT) process, which is possibly achieved by targeting DACH1. While downregulating miR-218 showed the opposite results. Conclusion: These data demonstrated that, under an in vitro HG environment, miR-218 suppressed the HK-2 cells proliferation, promoted apoptosis, caused an inflammatory response, and facilitated the EMT process largely by targeting DACH1, providing an insight into the therapeutic intervention of DKD.
- Published
- 2020