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Objective Epidemiological evidence investigating serum uric acid and kidney cancer risk remains unclear. We conducted this study to examine the relationship between serum uric acid and the incidence and mortality of kidney cancer. Methods This is a prospective analysis of 444 462 participants without any cancer from the UK Biobank. Serum uric acid was measured at baseline and the incidence and mortality of kidney cancer was determined through contact with the cancer and death registry. Cox regression models were fitted to estimate the hazard ratio (HR) and 95% confidence interval (95%CI), adjusting for demography, lifestyle style, comorbidities, and medication use. Results We documented 638 incidence cases and 188 mortality cases of kidney cancer over a median of 6.5 years follow‐up. People with the highest quartile had a 45% increased risk of kidney cancer compared to those with the lowest uric acid quartile (HR 1.45, 95%CI 1.08 to 1.93). Subgroup analyses showed that serum uric acid was associated with cancer risk among females but not among males (Q1 vs Q4: females HR1.47, 95%CI 1.01 to 2.16; males HR 1.19, 95%CI 0.91 to 1.56). Although we found serum uric acid was associated with an increased risk of kidney cancer mortality in age‐stratified model (HR 2.49, 95% CI 1.61 to 3.84), this association disappeared after further adjustment for other confounders. Conclusions High uric acid is associated with a high incidence of kidney cancer, especially in women. More research is needed to confirm our findings.

Aims: The aim was to describe the incidence of atrial fibrillation (AF) after cavotricuspid isthmus (CTI) ablation in patients with typical atrial flutter (AFL) without history of AF and to identify risk factors for new-onset AF after the procedure.Methods: A total of 191 patients with typical AFL undergoing successful CTI ablation were enrolled. Patients who had history of AF, structural heart disease, cardiac surgery, or ablation or who received antiarrhythmic drug after procedure were excluded. Clinical and electrophysiological data were collected.Results: There were 47 patients (24.6%) developing new AF during a follow-up of 3.3 ± 1.9 years after CTI ablation. Receiver operating characteristic (ROC) curves indicated that the cut-off values of left atrial diameter (LAD) and CHA2DS2-VASc score were 42 mm and 2, with area under the curve of 0.781 and 0.550, respectively. The multivariable Cox regression analysis revealed that obstructive sleep apnea (OSA) [hazard ratio (HR) 3.734, 95% confidence interval (CI) 1.470–9.484, P = 0.006], advanced interatrial block (aIAB) (HR 2.034, 95% CI 1.017–4.067, P = 0.045), LAD > 42 mm (HR 2.710, 95% CI 1.478–4.969, P = 0.001), and CHA2DS2-VASc score > 2 (HR 2.123, 95% CI 1.118–4.034, P = 0.021) were independent risk factors of new-onset AF.Conclusion: A combination of OSA, aIAB, LAD > 42 mm, and CHA2DS2-VASc > 2 was a strongly high risk for new-onset AF after ablation for typical AFL, and it had significance in postablation management in clinical practice.

Background: Several methods have been reported for locating the conduction gap (CG) in the pulmonary vein isolation (PVI) ablation line. However, the value of the interval between far-field atrial potential (FFP) and pulmonary vein potential (PVP) remains unknown.Methods: Consecutive patients with a CG during observation on the table after PVI were included. The PVP, FFP, and the CG location were evaluated to develop a novel algorithm to identify the CG location in the left superior pulmonary vein. The performance of this novel algorithm was prospectively tested in a validation cohort of consecutive patients undergoing repeat PVI ablation.Results: A total of 116 patients with atrial fibrillation (AF) were recruited, 56 of whom formed the validation cohort. The interval between FFP and PVP of the left superior pulmonary vein was associated with the CG location, and an interval Conclusions: The interval between FFP and PVP is a novel and accurate index that can be used to predict the CG location in the left superior pulmonary vein. An far-field atrial potential and pulmonary vein potential (FFP–PVP) interval value of ≥5 ms could be used to exclude a CG in the upper portion of the ostium in the majority of patients undergoing AF ablation.

Aim: The aim of this study was to investigate the relationship between CXCL-6 levels in wound exudates and healing of diabetic foot ulcers (DFU). Materials & methods: One hundred patients with neuropathic DFU were recruited. Wound exudate CXCL-6 levels were measured by enzyme-linked immunosorbent assay. Patients were followed for 24 weeks and divided into rapidly healing and nonhealing groups. Results: Compared with the NH group, the mean CXCL-6 levels in the wound exudates of the rapidly healing group were significantly higher. After adjusting for traditional risk factors, wound exudate CXCL-6 levels were still significantly associated with wound healing. Conclusion: CXCL6 is an independent predictor of wound healing in DFU patients and may be a potentially novel therapeutic target for the treatment of DFU.

Background: Common ostium of inferior pulmonary veins (COIPV) is a kind of pulmonary vein variation. The safety and efficacy of COIPV isolation using the second-generation cryoballoon (CB) ablation remain unknown.Methods: A total of 10 patients with COIPV from a consecutive series of 1,751 patients with atrial fibrillation (AF) were included. Pulmonary vein isolation (PVI) was performed using the second-generation CB.Results: The prevalence of a COIPV was 0.57% in this study. PVI was achieved in all pulmonary veins (PVs) without the need for a touch-up. A segmental freeze strategy was applied for each inferior PV, respectively. The mean number of freeze cycles of inferior PVs was 1.4 ± 0.5 for the left inferior pulmonary vein (LIPV), and 2.0 ± 0.9 for the right inferior pulmonary vein (RIPV). Pulmonary vein potential (PVP) of RIPV could not be monitored in real-time in three cases. Eight of 10 patients (80%) were free from atrial arrhythmias without the use of antiarrhythmic drugs during a follow-up period of 23.6 ± 12.9 months. No procedure-related complications occurred in any of the 10 patients.Conclusions: Common ostium of inferior pulmonary veins is a rare but challenging PV variant. PVI with this unusual anatomic variation using the second-generation 28-mm CB is effective and safe.

Diabetic cardiomyopathy (DCM) is a common diabetic complication characterized by diastolic relaxation abnormalities, myocardial fibrosis and chronic heart failure. Although TGF‐β/Smad3 signalling has been shown to play a critical role in chronic heart disease, the role and mechanisms of Smad3 in DCM remain unclear. We reported here the potential role of Smad3 in the development of DCM by genetically deleting the Smad3 gene from db/db mice. At the age of 32 weeks, Smad3WT‐db/db mice developed moderate to severe DCM as demonstrated by a marked increase in the left ventricular (LV) mass, a significant fall in the LV ejection fraction (EF) and LV fractional shortening (FS), and progressive myocardial fibrosis and inflammation. In contrast, db/db mice lacking Smad3 (Smad3KO‐db/db) were protected against the development of DCM with normal cardiac function and undetectable myocardial inflammation and fibrosis. Interestingly, db/db mice with deleting one copy of Smad3 (Smad3 ± db/db) did not show any cardioprotective effects. Mechanistically, we found that deletion of Smad3 from db/db mice largely protected cardiac Smad7 from Smurf2‐mediated ubiquitin proteasome degradation, thereby inducing IBα to suppress NF‐kB‐driven cardiac inflammation. In addition, deletion of Smad3 also altered Smad3‐dependent miRNAs by up‐regulating cardiac miR‐29b while suppressing miR‐21 to exhibit the cardioprotective effect on Smad3KO‐db/db mice. In conclusion, results from this study reveal that Smad3 is a key mediator in the pathogenesis of DCM. Targeting Smad3 may be a novel therapy for DCM.

Background Ventricular arrhythmias (VAs) originating from ventricular outflow tracts can possess a high similarity of QRS configuration resulting in an inaccurate localization, while the reminder of mapping or ablation in the lower right ventricular outflow tract (RVOT) commonly provide the preferred transformation strategy. Methods We retrospectively analyzed the total of 958 patients who were referred for radiofrequency (RF) ablation of VAs in our center. VAs with the sinus of valsalva (SoV) origins were enrolled. Results A total of 120 consecutive patients (mean age 45.0 ± 15.5 years) undergoing the ablation of VAs arising from the left-sided ventricular outflow tracts were included, with 55 (45.8%) female. All patients had a QRS morphology of the LBBB pattern and an inferior axis, with the mean earliest ventricular activation (EVA) of target site of 34.9 ± 8.8ms. 37 (30.8%), 60 (50.0%), and 23 (19.2%) patients obtained the successful RF ablation at the right sinus cusp (RCC), left sinus cusp (LCC), and the commissure of RCC and LCC (R-LCC), respectively. 62 (51.7%) target sites of SoV could record a high-frequency potential. Moreover, 25 (20.8%) patients were continuously recorded an early ventricular activation in the lower RVOT and achieved the elimination of VAs in SoV. Conclusions VAs originating from the SoV could show a low specificity of QRS morphology mimicking the RVOT-like ECG feature. An early ventricular activation mapped in the lower RVOT or RF ablation with poor response likely demonstrated the VAs originating from the SoV, especially the RCC.

Background: A common ostium of inferior pulmonary veins (COIPV) is a kind of unusual pulmonary veins variation. Limited data are available about the safety and efficacy using the second‐generation cryoballoon (CB) ablation in patients with atrial fibrillation with such an anatomical variation. Methods: A total of 10 (0.57%) patients with a common ostium of inferior pulmonary veins from a consecutive series of 1751 consecutive patients with atrial fibrillation (AF) were included. Pulmonary vein isolation was performed using the second‐generation CB. Results: PV isolation was successfully achieved in all PVs without the need of additional focal catheter procedures. CB could occlude and cryotherapy each inferior PV respectively. The mean number of freeze cycles of inferior PVs in each patient was 2.5±1.6, and 2.1±0.9, respectively. The mean time to isolation and nadir temperature were 54.3±22.1, 53.2±22.2 seconds and -39.7±3.4°C, -43.9±7.5°C, respectively. No procedure-related complications occurred in any of the 10 patients. 8 of 10 patients (80%) had no atrial arrhythmia recurrences without the use of antiarrhythmic drugs during a follow-up period of 24±13 months. Conclusion: The incidence of a common ostium of inferior pulmonary veins is 0.57% in present study. PV isolation with this unusual anatomic variation using the second‐generation 28‐mm CB is effective and safe.

BACKGROUND Several methods have been proposed to locate the conduction gap on the pulmonary vein isolation (PVI) ablation line. However, the value of the interval of far-field potential (FF potential) and pulmonary vein potential (PV potential) is unknown. METHODS: The state of PV potential and the location of that ablation sites were particularly recorded for analysis once LSPV was isolated in a development cohort of consecutive patients undergoing PVI ablation at a single center. These findings informed the development of a novel algorithm to discriminate the location of a conduction gap between upper portion and inferior portion of LSPV. The performance of this novel algorithm was prospectively tested in a validation cohort of consecutive re-do patients undergoing PVI ablation RESULTS: A total one hundred sixteen patients were recruited, of which 56 formed the validation cohort. Interval of FF potential and PV potential was associated with the location of the conduction gap, and the interval

Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterised by communication disability with no curative treatment. Maternal separation-induced ultrasonic vocalisation (USV) was widely used to assess communication disability between pups and dams. Particularly, USV calls in many genetically modified ASD model mice were altered. Previously, we demonstrated that mice pups exposed to valproic acid in utero (VPA pups) showed decreased number of USV calls on postnatal day 11 and were rescued by subcutaneous injection of oxytocin. However, the qualitative change of USV calls by oxytocin has not been evaluated in VPA pups. In the present study, we examined the duration of oxytocin effect and analysed the altered pattern of USV calls using VPA pups. The oxytocin administration increased the total number of USV calls and the effect persisted up to 120 min in VPA pups. The pattern analysis revealed that the increase in the number of complex calls also persisted up to 120 min. These results suggested that oxytocin had a prolonged effect on USV calls, mainly on complex calls, in VPA pup, showing that oxytocin could recover their social modality to respond to maternal separation.

Critical Limb Ischemia (CLI) is common but uncommonly diagnosed. Improved recognition and early diagnostic markers for CLI are needed. Therefore, the aim of our study was to identify plasma biomarkers of CLI in patients with type 2 diabetes mellitus (T2DM). In this study, antibody-coated glass slide arrays were used to determine the plasma levels of 274 human cytokines in four matched cases of diabetes with and without CLI. Potential biomarkers were confirmed in an independent cohort by ELISA. After adjusting for confounding risk factors, only plasma level of Siglec-5 remained significantly associated with an increased odds ratio (OR) for diabetes with CLI by binary logistic regression analysis. Receiver operating characteristic (ROC) curve analysis revealed the optimal cut-off points for Siglec-5 was 153.1 ng/ml. After entering Siglec-5, the AUC was 0.99, which was higher than that of confounding risk factors only (AUC = 0.97, P

The blade deformation caused by aerodynamic and centrifugal loads during operating makes blade configurations different from their stationary shape. Based on the load incremental approach, a novel pre-deformation method for cold blade shape is provided in order to compensate blade deformation under running. Effect of nonlinear blade stiffness is considered by updating stiffness matrix in response to the variation of blade configuration when calculating deformations. The pre-deformation procedure is iterated till a converged cold blade shape is obtained. The proposed pre-deformation method is applied to a transonic compressor rotor. Effect of load conditions on blade pre-deformation is also analyzed. The results show that the pre-deformation method is easy to implement with fast convergence speed. Neither the aerodynamic load nor centrifugal load can be neglected in blade pre-deformation.

Background Chemotherapy induced diarrhea (CID) is a common side effect in patients receiving chemotherapy for cancer. The aim of our study was to explore the association between gut microorganisms and CID from the CapeOX regimen in resected stage III colorectal cancer (CRC) patients. Results After screening and identification, 17 stool samples were collected from resected stage III CRC patients undergoing the CapeOX regimen. Bacterial 16S ribosomal RNA genes was sequenced, and a bioinformatics analysis was executed to screen for the distinctive gut microbiome and the functional metabolism associated with CID due to the CapeOX regimen. The gut microbial community richness and community diversity were lower in CID (p 2), and there were 23 pathways that the differential microorganisms might be involved in. Conclusions The gut microbial community structure and diversity have changed in CRC patients with CID. It may provide novel insights into the prevention and treatment of CID. Electronic supplementary material The online version of this article (10.1186/s13099-019-0299-4) contains supplementary material, which is available to authorized users.

Objectives. Chronic foot ulceration is a severe complication of diabetes, driving morbidity and mortality. The aim of our study was to identify novel biomarkers of impaired wound healing in diabetic foot ulcers. Methods. 109 patients with neuropathic diabetic foot ulcers and 30 burn victims otherwise healthy participated. Antibody-coated glass slide arrays were used to determine the levels of 80 human cytokines in pooled plasma or pooled wound exudate of diabetic foot ulcers with rapidly healing (RH, n=12) and matched nonhealing (NH, n=12) patients. Potential biomarkers were confirmed in an independent cohort by enzyme-linked immunosorbent assay (ELISA). Results. Protein array profiling identified 27 proteins or 15 proteins significantly altered in protein profiling of pooled plasma or pooled wound exudate of 12 RH patients compared with 12 matched NH patients, respectively. In an independent cohort, quantitative ELISA validation confirmed a decrease in MCP-2 and ENA-78 levels in NH patients versus RH patients or burn victims. After adjusting for the traditional risk factors (sex, age, body mass index, fasting plasma glucose, ulcer area, HbA1C, diabetes duration, hyperlipidemia, and antibiotic therapy), only wound exudate level of ENA-78 remained having a significant association with an increased odds ratio (OR) for wound healing by binary logistic regression analysis (P<0.05). Conclusion. Decreased wound exudate ENA-78 was independently associated with wound healing of patients with diabetic foot. Exudate ENA-78 level is implicated as a novel predictor of wound healing in patients with diabetic foot ulcers.

In recent years, group B streptococcus (GBS) has become an important pathogen that causes infections in many neonatal organs, including the brain, lung, and eye (Ballard et al., 2016). A series of studies performed on GBS infections in western countries have revealed that GBS is one of the primary pathogens implicated in perinatal infection, and GBS infections are a major cause of neonatal morbidity and mortality in the United States (Decheva et al., 2013). In China, GBS is mainly found by screens for adult urogenital tract and perinatal infections, and neonatal GBS infections have been rarely reported. The incidence rate of early-onset neonatal GBS disease is thought to be lower in China than in western countries; however, this data is controversial since it also reflects the clinical interest in GBS (Dabrowska-Szponar and Galinski, 2001).

The purpose of this study was to investigate the effect of glycaemic control on subclinical hypothyroidism (SCH) in Chinese type 2 diabetic patients.The study included 476 diabetic patients with SCH admitted for treatment of type 2 diabetes. The controls were selected euthyroid patients with similar characteristics regarding [age, body mass index (BMI), gender, previous hypertension or duration of diabetes, and smoke]. Total cholesterol (TC), LDL cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), the homeostatic model assessment of insulin resistance ratio (HOMA-IR), fasting plasma glucose, hemoglobin AThe LDL-C level and HOMA-IR value were significantly higher in the SCH group. Glycaemic control reduced HOMA-IR, HbAOur study shows that glycaemic control may bring some benefits to type 2 diabetic patients with SCH.

The study aimed to determine the relationships between the basal metabolic rate (BMR) and body composition of overweight and obese Chinese adults with type 2 diabetes mellitus (DM). This cross-sectional clinical study enrolled 193 Chinese adults with type 2 DM who were overweight (24 kg/m(2)=BMI≤28 kg/m(2), n=99), or obese (BMI ≥28 kg/m(2), n=94). Ninety-seven adults with normal BMIs, including 50 DM patients and 47 healthy adults, were recruited as a control group. BMR was measured by indirect calorimetry; predicted BMR was calculated according to the Schofield equation; and the relationships between BMR, body composition, and biochemical results were determined by the Pearson correlation. The results showed that obese DM patients had significantly higher BMRs than both overweight patients (P

The influence of low-mode-driven asymmetry on yield degradation in shaped-pulse indirect-drive implosions has been investigated at the 100 kJ laser facility. In this work, P2- and P4-driven asymmetries were tuned by varying hohlraum gas-fill density and capsule diameter. The measured neutron yield varied from 2.1 × 109 to 7.6 × 109 and the yield measured by a 1D simulation (YOC1D) was increased from 3% to 16%. Meanwhile, considering the temporal P2- and P4-driven asymmetries, the yields measured by 2D simulations (YOC2D) were from 26% to 81% (the YOC2D of the majority of the shots was higher than 50%). Furthermore, both the ion temperature and neutron bang-time showed good agreement between the measurements and the 2D simulations. The simulations demonstrated that the temporal P2- and P4-driven asymmetries can decrease the efficiency of PdV work and increase the energy loss due to electron thermal conduction. In addition, the internal energy of the deuterium (DD) fuel clearly decreased and the neutron yield was degraded by low-mode asymmetry. In a future work, we will enlarge the cylindrical hohlraum diameter or use an I-hohlraum to improve the low-mode-driven symmetry, along with measurement of the hot-spot shape.

We recently reported that transplantation of autologous bone marrow mononuclear cells (BM-MNCs) may be an effective and promising therapy to treat refractory diabetic sensorimotor polyneuropathy (DSPN) in patients with type 2 diabetes mellitus (T2DM). This study was designed to investigate the potential mechanisms of BM-MNCs therapy, which recruited 60 patients with DSPN, 30 T2DM patients without complications, and 30 healthy control participants. All clinical parameters, the levels of inflammatory markers, and growth factors in the three groups were compared. Patients in DSPN group had higher level of tumor necrosis factor-α (TNF-α) (DSPN vs control, 412.90 ± 64.58 vs 374.81 ± 63.18 pg/mL, P < 0.01) and lower level of vascular endothelial growth factor (VEGF) (DSPN vs control, 140.93 ± 24.78 vs 157.39 ± 25.11 pg/mL, P < 0.01) than those in control group. DSPN group had the highest level of soluble intercellular adhesion molecule-1 (sICAM-1) among three groups (DSPN and DM vs control, 1477.56 ± 228.00 and 1342.17 ± 237.54 vs 1308.00 ± 200.94 ng/mL, P < 0.05). The level of nerve growth factor in the DSPN group was slightly lower than that in the DM group (DSPN vs DM, 3509.11 ± 438.39 vs 3734.87 ± 647.50 pg/mL, P < 0.05). All patients with DSPN received one intramuscular injection of BM-MNCs and clinical follow-ups after the therapy for 2 days, 1, 4, 12, 24, and 48 weeks. Neuropathic symptoms of foot pain, numbness, and weakness were significantly improved within 4 weeks after BM-MNCs injection. Patients with DSPN were divided into the responder ( n = 35) and nonresponder groups ( n = 19) based on the improvement of nerve conduction velocity at 12 weeks post-transplantation. Compared with nonresponders, responders were younger (57.3 ± 5.2 vs 62.0 ± 4.8, P < 0.01), had a shorter history of diabetes (7.1 ± 2.7 vs 11.2 ± 5.4 years, P < 0.01), and had higher numbers of mobilized CD34+cells (17.61 ± 2.64 vs 14.79 ± 1.62 ×105/L, P < 0.01) and BM-MNCs (12.05 ± 2.16 vs 9.84 ± 1.53 ×108/L, P < 0.01). The levels of TNF-α and sICAM-1 decreased just after BM-MNCs injection in both groups and slowly reverted to baseline levels. The duration of the downtrend of TNF-α and sICAM-1 in the responder group lasted longer than that in the nonresponder group. Serum level of VEGF in the responder group increased immediately after BM-MNC therapy and reached the highest point after the injection for 12 weeks. On the other hand, VEGF levels in the nonresponder group only increased slightly. Binary logistic regression was performed to evaluate the corresponding prognostic factors for BM-MNCs treatment. The number of applied CD34+cells and the duration of diabetes were the independent predictors of responding to BM-MNCs therapy. No adverse event associated with the treatment was observed during follow-up observations. These results indicated that BM-MNCs transplantation is an effective and promising therapeutic strategy to treat refractory DSPN. The immune regulation and paracrine function of BM-MNCs may contribute to the improvement of DSPN.Trial registration:Chinese Clinical Trial Registry, ChiCTR-TRC-12002570. URL: http://www.chictr.org.cn/showproj.aspx?proj=6981

Objective Clinical sepsis-associated biomarkers were utilized in a cecal ligation and puncture (CLP) septic mouse model to provide a reference for investigating pathophysiological mechanisms and evaluating novel therapeutic interventions for sepsis. Methods Sepsis in mice was induced by CLP, and clinical biomarkers were evaluated (survival rate, blood physiological and biochemical indices, cytokines, hepatorenal function parameters, and blood coagulation). Results The mortality rate was >70%. The body temperature, blood pressure, and heart rate decreased within 48 h. Low lactic acid was found at 8 h. The CLP mice showed typical inflammatory symptoms with decreased white blood cells and procalcitonin and increased levels of soluble triggering receptor expressed on myeloid cells-1, interleukin (IL)-6, IL-10, tumor necrosis factor-α, macrophage inflammatory protein (MIP)-1α, MIP-1β, and MIP-2. The platelet count and activated partial thromboplastin time significantly decreased, and the prothrombin time and prothrombin time–international normalized ratio markedly increased. Phenotypes of multiple organ dysfunction were found in the CLP model, including increased liver alanine aminotransferase and aspartate transaminase; significantly reduced total protein, globulin, and serum albumin; increased blood urea nitrogen and creatinine; and decreased blood glucose. Conclusion The clinical features of the CLP mouse model were similar to those of human patients with sepsis.

The aim of this study was to investigate whether osteopontin(OPN)variants are associated with susceptibility to ankylosing spondylitis (AS) in a Chinese population. Polymorphisms at the 9175th position in exon 7 ofOPNand rs17524488 were genotyped using direct sequencing in 186 unrelated AS patients and 188 ethnically matched healthy controls. Serum concentration of OPN was measured by enzyme-linked immunosorbent assay (ELISA) in all participants. AS patients displayed significantly higher OPN serum levels than the controls (P<001). A heterozygous, novel 9175 T>A in exon 7 of theOPNgene was found in this study. In healthy controls, subjects carrying the rs17524488 G/G genotype of theOPNdisplay significantly higher OPN serum levels than the GG/GG genotype (P<0.05). Plasma OPN level is implicated as an early diagnostic marker of AS. The novel 9175th- (exon 7) position polymorphism ofOPNand rs17524488 were related to susceptibility to AS in a Chinese population, the rs17524488 G/G genotype may be involved in the pathogenesis of AS, and the precise molecular mechanism underlying the influence ofOPNpolymorphisms on the development of AS remains to be determined in the further prospective studies.

Critical limb ischemia (CLI) is the most severe manifestation of peripheral artery disease, which is common but rarely diagnosed. Noninvasive biomarkers are urgently required to assist in the diagnosis of CLI. Accumulating evidence indicates that miRNAs play an important role in the development of various diseases. In this study, microarray profiling revealed 11 miRNAs with significantly altered expression in four T2DM patients with CLI compared with that in four sex- and age-matched T2DM patients without CLI. In independent cohorts, qRT-PCR validation confirmed the increased miRNA-4739 level in patients with CLI versus patients without CLI. miRNA-4739 levels increased with FPG and HbA1c (all P < 0.05). After adjusting for the risk factors, miRNA-4739 levels were found to be associated with an increased odds ratio (OR) of T2DM with CLI (OR =12.818, 95% confidence intervals (CI) 1.148 to 143.143, P = 0.038). ROC curve analysis revealed that the area under the curve (AUC) of miR-4739+confounding risk factors was 0.94 (95% CI 0.891 to 0.998, P < 0.001), which was higher than that of confounding risk factors (AUC 0.94 vs. 0.91, 95% CI -0.122 to 0.060, P > 0.05) and of miR-4739 (AUC 0.94 vs. 0.69, 95% CI -0.399 to -0.101, P < 0.001), respectively. We conclude that elevated plasma miRNA-4739 levels are independently associated with CLI in T2DM patients. miRNA-4739 is implicated as a novel diagnostic marker and a potential therapeutic target for CLI in diabetes.

The surface wave generated by flow around a ship hull moving near free surface of water is simulated numerically in this study. The three-dimensional implicit finite volume method (FVM) is applied to solve Reynolds averaged Navier-Stokes (RANS) equation. The realizable k-e turbulence model has been implemented to capture turbulent flow around the ship hull in the free surface zone. The volume of fluid (VOF) method coupled with the Stokes wave theory has been used to determine the free surface effect of water. By using is a six degrees of freedom model, the ship hull’s movement is numerically solved with the Stokes wave together. Under the action of Stokes waves on the sea, the interface between the air and water waves at the same regular pattern and so does the pressure and the vertical velocity. The ship hull moves in the same way as the wave. The amplitude of the ship hull’s heave is less than the wave height because of the viscosity damping. This method could provide an important reference for the study of ships’ movement, wave and hydrodynamics.

In this paper, we combine the practical application of my school digital campus construction, explores the construction of campus smart card under wisdom campus-based platform in our college. Select non-contact logic encryption IC card. Analysis the overall objectives and system functional requirements of the wisdom campus smart card system, present a system function chart. Finally, combine with cooking food department, which is the characteristic profession of our tourism school, system analysis and design a training room management subsystem in campus smart card system.

The development of modern network and information technology makes the construction of wisdom campus based on the network become an important topic in the development of education information. This paper constructs the overall framework of intelligent campus based on the perception layer, network layer, data layer, service layer, application layer, terminator layer and user layer. The framework’s functions contain information collection, network integration, data integration, service integration and unified portal service. At the same time, it uses information technology standard system and security guarantee system as a support system and uses the cloud computing and Internet of things as the key technology to preliminary study on the construction of wisdom campus based on network.

Objective To investigate whether height is associated with peripheral arterial disease (PAD) in patients with type 2 diabetes. Research design and methods This was an observational study performed in 4,528 Chinese patients with type 2 diabetes. Anthropometric measures and the ankle-brachial index (ABI) were performed on each subject. PAD was defined as those patients with a history of revascularization or amputation due to ischemia, or an ABI

Based on the existing three mechanisms in the Carbon emission trading system, this paper proposes a hybrid carbon trading mechanism which is compatible with the characteristics of all the participants, analyzes the operation modes, benefits and risks of the power suppliers, builds a risk decision model for emission right trading according to the Financial Portfolio Theory, and gives its efficient frontier. This study provides not only theoretical supports for the power supplier’s effective management of emissions risks, but also expands the application scope of the risk management theory. Furthermore, this paper provides a reference for the government to establish an open carbon trading system.

In the environment of electricity and derivative transaction, the risk hedging and metastasis of power right transaction in China are needed to establish the risk decision-making for the generated energy/right on electricity generation side. And it will be the key point focused by decision-makers in power enterprises. The planning management of two-stage market transaction risk was conducted with the hedging function of generation rights transaction to establish a risk decision model of comprehensive electricity transaction. The relationship between the electricity price of two-stage market and the identity parameters of transaction participants was given for solving the model. The whole transaction process of generating companies was divided into two stages. The risk decision framework of block transaction was established for the independent transaction decision-making of each segment and the overall optimization. The results of example simulation verified the effectiveness and feasibility of the decisions.

As micro-grid technology's development, it has played an important role in improving the diversity and power quality of power supply. Micro-grid power supply planning is an important part of the micro-grid planning, the paper describes two different micro-grid power supply planning simulation logic, to provide some reference ideas for the micro-grid power supply planning simulation.

This paper establishes a power planning model based on minimization of the initial investment cost. The model includes the initial investment cost of wind turbines, photovoltaic cells, batteries, and biomass generators, diesel generators, power electronic devices and communications facilities in the objective function. In consideration of some constraint conditions, using the principle of priority of biomass generators, we use the particle swarm algorithm to solve the model. Through the calculation of actual examples, we simulate to find optimal solutions. Finally, we conclude that it should be possible to use biomass power generation and diesel generator power.

This paper explores the risk decision issue of the emission/right trading in power enterprises according to the various possible situations which may exist in the carbon trading system. Firstly, the emission environment is divided into three separate Cases and two combination (hybrid) Cases are virtualized. Secondly, the advantages and disadvantages for both the enterprises and the country are analyzed under the existing separate situation and the risk decision-making framework of the emission/right trading is established for the power generation companies. Finally, a trading risk decision model is constructed in the combination Cases by using the financial asset portfolio theory and the effective frontier for the model is given. The model is broad and simple in application scope. It has some theoretical meanings and practical values. And it provides the operational space for the risk decision of the power enterprises.

This paper establishes a power planning model based on minimizing operation and maintenance cost. The model take into account the costs of the wind turbines, photovoltaic cells, batteries, biomass generators, diesel generators, power electronics devices and communications facilities operation and maintenance, credit them in the objective function, and considering some certain constraints, use particle swarm algorithm model, by calculating and, simulating examples , to find the optimal solution, Finally to find the conclusion that to use wind turbines, photovoltaic cells and batteries for electric as much as possible.

The development of micro-grid technology provides power system a variety of forms, flexible ways of power supply schemes, but a lot of basic micro-grid operational problems are different from the traditional large power grids, which need to be studied specifically. This paper studies the power planning problem of micro-grid, namely to meets the system operational constraints, study how to optimize the micro-power configuration of micro-grid, so that the whole micro-grid life cycle cost is the smallest.

Because the micro-grid investors, beneficiary, management mode, etc. are fairly different from those of traditional power grid. From the perspective of micro grid users or operators, this paper analyzes the operating and maintenance cost of power pack of multi-energy source hybrid power supply, designs the micro-grid power pack model of multi-energy source hybrid power supply by use of static planning measure, aiming at the minimization of operating cost, and analyzes the power pack configuration under the condition of different minimum operating costs by case study and how to achieve the operating and maintenance profits.

The reasonable deployment of micro-grid power supply determines the application domain and flexibility of micro grid. Because the micro-grid investors, beneficiary, management mode, etc. are fairly different from those of traditional power grid. From the perspective of micro grid users or operators, this paper analyzes the operating and maintenance cost of power pack of multi-energy source hybrid power supply, designs the micro-grid power pack model of multi-energy source hybrid power supply by use of static planning measure, aiming at the minimization of operating cost, and analyzes the power pack configuration under the condition of different minimum operating costs by case study and how to achieve the operating and maintenance profits and reliability.

This paper focuses on the composition of the Heavy-medium coal separation automatic control system, control methods and its functions. Introduced the main testing instruments and actuator in the control system. The system achieved good results in the Tangshan Rongyi Coal Preparation Company.

This work designs a novel hydraulic-walking incremental launching equipment. The working principle of the equipment is proposed. Considering that the contact in incremental launching construction is a typical elastic contact problem, and changes in contact state will directly affect the stability of the incremental launching, this work proposes the equilibrium equations of computational contact mechanics and numerical analysis method for the incremental launching equipment through analyzing the working process of the equipments. The proposed model and method are applied in the analysis of typical working conditions of the incremental launching of Donghua Bridge. The structural characteristics in the construction process are analyzed and discussed. The analysis results show that the strength and stability of the steel box girder can meet the construction requirements. The incremental launching of Donghua Bridge was completed successfully. The proposed model and method can be used to predict and analyze the structural performance in incremental launching construction as well as provide theoretical reference and analysis method for similar constructions.

Fundus images have been widely used in the diagnosis of retinopathy and cardiovascular diseases. However, because of the movement of patients' eyes and limitation of medical equipments, the quality of fundus images may be low sometimes. In this paper, we propose vessel enhancement method for a low-contrast and blurred image based on multi-scale morphological top-hat transformation, and the combination of Gabor and matched filter. The underlying rationale of this proposed method is to make use different kinds of information to improve blood vessels on retinal fundus images with poor quality. Experiments show that vessels enhanced by our proposed method are much clearer than the ones using original multi-scale morphological top-hat transformation, Gabor filter or matched filter. The results confirm that the quality of vessel enhancement can be improved by combining these methods.

AIM To evaluate the advantage of canaloplasty compared to trabeculectomy for patients with open angle glaucoma. METHODS Potentially relevant studies were systematically searched using various databases from inception until December 2015. The outcome analyses performed automatically using Revman 5.3 included intraocular pressure reduction (IOPR), postoperative success rate, anti-glaucoma medications reduction and the incidence of adverse events. RESULTS We included four qualified studies incorporating a total of 215 eyes for quantitative synthesis. The weighted mean difference (WMD) of IOPR between canaloplasty and trabeculectomy from baseline to 12mo was -2.33 (95%CI: -4.00, -0.66). There was not significant improvement in the complete or qualified success rate (OR: 0.58, 95%CI: 0.26, 1.31; OR: 0.50, 95%CI: 0.10, 2.44, respectively). Similarly, no statistically significance was observed in anti-glaucoma mediations reduction (WMD: -0.54, 95%CI: -1.18, 0.09). Sensitivity analysis of the primary outcome estimate confirmed the stability of the Meta-analysis result. CONCLUSION Trabeculectomy seems to be more effective in lowering IOP up to 12mo when comparing with canaloplasty. Canaloplasty does not seem to be inferior to trabeculectomy considering the postoperative success rate or the number of postoperative anti-glaucoma medications. Meanwhile, it has an advantage of less bleb related complications.

A kind of intelligent magnetic starter was introduced. It can control the starting, maintaining and breaking of the AC contactor. The function of protector was to protect the motor when it was in the case of over-voltage, under-voltage, jam, overload, unbanlance and phase loss. It can also display the line voltage, three phase current of the motor, Moreover, the intercommunication between the protector and PC was realized.