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Poverty reduction is an important driving force for the global realization of the United Nations 2030 Sustainable Development Goals and the building of a global community with a shared future for mankind. This paper takes the Belt and Road Initiative (BRI) as a quasi-natural experiment. Based on the panel data of 134 countries in the world from 2000 to 2018, this paper uses the difference-in-difference (DID) model to examine the impact of the BRI on poverty reduction in countries along the route and to study its internal mechanism from the dual perspectives of trade openness and investment openness. The results show that (1) the BRI can significantly reduce the incidence of poverty with the impact coefficient stable at −0.26, and the empirical conclusion has passed the parallel test, placebo test, and instrumental variable test; (2) the mediating effect test model shows that the BRI can achieve poverty reduction through the dual openness of trade and investment, and the intermediary effect of trade openness is greater than investment openness; and (3) from the perspective of heterogeneity analysis, geographically, the BRI has a slightly higher role in promoting poverty reduction in landlocked countries than in coastal countries. In terms of economic location, the effect of poverty reduction has a certain "pro-poverty" characteristic, that is, the BRI promotes poverty reduction in low- and middle-income countries far more than other types of income countries. Therefore, we believe that the continuous deepening of the BRI high-quality construction and the strengthening of cooperation among countries along the route will play a key role in promoting the international cycle of trade, investment, and other factors, as well as the cause of poverty reduction in the region and the world. [ABSTRACT FROM AUTHOR]