1. Comprehensive Analysis of Differentially Expressed Long Noncoding RNA-mRNA in the Adenoma-Carcinoma Sequence of DNA Mismatch Repair Proficient Colon Cancer
- Author
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Wu Lin, Nanshan Li, Kuiliang Liu, Hong Liu, Yadan Wang, Qian Li, Jiang Ge, Chunmei Guo, Jing Wu, Canghai Wang, Wen-Kun Li, Yun Wang, Guojun Jiang, Yueqiong Lao, and Nan Wei
- Subjects
0301 basic medicine ,Intraepithelial neoplasia ,Article Subject ,business.industry ,Microarray analysis techniques ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Computational biology ,medicine.disease_cause ,Long non-coding RNA ,law.invention ,Transcriptome ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Oncology ,law ,030220 oncology & carcinogenesis ,Medicine ,DNA mismatch repair ,KEGG ,business ,Carcinogenesis ,RC254-282 ,Polymerase chain reaction ,Research Article - Abstract
DNA proficient mismatch repair colon cancer (pMMR CC) is the most common subtype of sporadic CC. We aimed to investigate the role of long noncoding RNAs (lncRNAs) in pMMR CC carcinogenesis. In the present study, we conducted transcriptomic analysis of lncRNAs-mRNAs in five low-grade intraepithelial neoplasia (LGIN), five high-grade intraepithelial neoplasia (HGIN), four pMMR CC, and five normal control (NC) tissues. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway, and coexpression network analyses were performed to elucidate the functions of lncRNAs and mRNAs as well as their interactions. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate five dysregulated lncRNAs in a large set of colon tissues. Receiver-operating characteristic (ROC) curves were employed to evaluate the performance of the candidate lncRNAs. A set of 5783 differentially expressed lncRNAs and 4483 differentially expressed mRNAs were detected among the LGIN, HGIN, pMMR CC, and NC samples. These differentially expressed lncRNAs and mRNAs were assigned to 275 significant GO terms and 179 significant KEGG enriched pathways. qRT-PCR confirmed that the expression of five selected lncRNAs (ENST00000521815, ENST00000603052, ENST00000609220, NR_026543, and ENST00000545920) were consistent with the microarray data. ROC analysis showed that four lncRNAs (ENST00000521815, ENST00000603052, ENST00000609220, and NR_026543) had larger area under the ROC curve (AUC) values compared to serum carcinoembryonic antigens, thereby distinguishing NC from pMMR CC. In conclusion, several lncRNAs play various roles in the adenoma-carcinoma sequence and may serve as potential biomarkers for the early diagnosis of pMMR CC.
- Published
- 2021