1. Multiplexed MRM-based protein quantification of putative prognostic biomarkers for chronic kidney disease progression in plasma
- Author
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Àngel Argilés, Vasiliki Lygirou, Eleni Petra, Flore Duranton, Harald Mischak, Manousos Makridakis, Jerome Zoidakis, Szymon Filip, Antonia Vlahou, Georgia Kontostathi, and Rafael Stroggilos
- Subjects
Male ,0301 basic medicine ,Oncology ,030232 urology & nephrology ,lcsh:Medicine ,Disease ,Mass Spectrometry ,Cohort Studies ,Prognostic markers ,0302 clinical medicine ,Multiplex ,Longitudinal Studies ,lcsh:Science ,Aged, 80 and over ,Multidisciplinary ,Immunoglobulin heavy constant alpha 1 ,Middle Aged ,Prognosis ,Blood proteins ,Hemoglobin Subunits ,Disease Progression ,Female ,medicine.symptom ,Complement C1 Inhibitor Protein ,Glomerular Filtration Rate ,Adult ,medicine.medical_specialty ,Quantitative proteomics ,Proteomic analysis ,Renal function ,Article ,03 medical and health sciences ,Internal medicine ,Alpha-Globulins ,medicine ,Humans ,Renal Insufficiency, Chronic ,Aged ,business.industry ,lcsh:R ,medicine.disease ,030104 developmental biology ,Albuminuria ,Muramidase ,lcsh:Q ,beta 2-Microglobulin ,business ,Biomarkers ,Follow-Up Studies ,Kidney disease - Abstract
Current diagnostic measures for Chronic Kidney Disease (CKD) include detection of reduced estimated glomerular filtration rate (eGFR) and albuminuria, which have suboptimal accuracies in predicting disease progression. The disease complexity and heterogeneity underscore the need for multiplex quantification of different markers. The goal of this study was to determine the association of six previously reported CKD-associated plasma proteins [B2M (Beta-2-microglobulin), SERPINF1 (Pigment epithelium-derived factor), AMBP (Protein AMBP), LYZ (Lysozyme C), HBB (Hemoglobin subunit beta) and IGHA1 (Immunoglobulin heavy constant alpha 1)], as measured in a multiplex format, with kidney function, and outcome. Antibody-free, multiple reaction monitoring mass spectrometry (MRM) assays were developed, characterized for their analytical performance, and used for the analysis of 72 plasma samples from a patient cohort with longitudinal follow-up. The MRM significantly correlated (Rho = 0.5–0.9) with results from respective ELISA. Five proteins [AMBP, B2M, LYZ, HBB and SERPINF1] were significantly associated with eGFR, with the three former also associated with unfavorable outcome. The combination of these markers provided stronger associations with outcome (p
- Published
- 2020