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Mounting evidence has shown that long noncoding RNAs (lncRNAs) play critical roles in carcinogenesis and tumor progression. SNHG12 has been identified in multiple types of malignant tumors. However, the role of SNHG12 in human non‐small cell lung cancer (NSCLC) is poorly characterized, and the relevant underlying mechanism remains unclear. The expression levels of SNHG12, miR‐101‐3p, and CUL4B in collected human NSCLC tumor tissues and NSCLC cell lines were tested via qRT‐PCR. Then, NSCLC cellular proliferation, migration and invasion were determined, followed by MTT, scratch and Transwell assays. Dual‐luciferase reporter assays and RNA pulldown assays were adopted to explore the target site. Moreover, western blotting was performed to detect the relevant protein expression concerning the CUL4B/PI3K/AKT pathway. This study clarified that SNHG12 knockdown significantly reduced proliferation, migration, invasion and EMT of NSCLC cells. Our data indicated that SNHG12 targeted and negatively regulated miR‐101‐3p, and this depletion reversed the inhibitory effect of si‐SNHG12 on NSCLC cells. Furthermore, CUL4B was confirmed as a functional target of miR‐101‐3p, and its knockdown resulted in a strong alleviation of the NSLCL cell phenotype, which was enhanced by the silencing of miR‐101‐3p. Mechanistically, we found that SNHG12 regulated miR‐101‐3p to modulate the PI3K/AKT pathway mediated by CUL4B.These observations suggested that lncRNA SNHG12‐mediated miR‐101‐3p downregulation regulated the malignant phenotype of NSCLC cells by targeting CUL4B through the PI3K/AKT pathway, which may present a path to novel therapeutic strategies for NSCLC therapy.

Aortic dissection (AD) is a catastrophic cardiovascular emergency with a poor prognosis, and little preceding symptoms. Abnormal lipid metabolism is closely related to the pathogenesis of AD. However, comprehensive lipid alterations related to AD pathogenesis remain unclear. Moreover, there is an urgent need for new or better biomarkers for improved risk assessment and surveillance of AD. Therefore, an untargeted lipidomic approach based on ultra-high-performance liquid chromatograph-mass spectrometry was employed to unveil plasma lipidomic alterations and potential biomarkers for AD patients in this study. We found that 278 of 439 identified lipid species were significantly altered in AD patients (n = 35) compared to normal controls (n = 32). Notably, most lipid species, including fatty acids, acylcarnitines, cholesteryl ester, ceramides, hexosylceramides, sphingomyelins, lysophosphatidylcholines, lysophosphatidylethanolamines, phosphatidylcholines, phosphatidylinositols, diacylglycerols, and triacylglycerols with total acyl chain carbon number ≥54 and/or total double bond number ≥4 were decreased, whereas phosphatidylethanolamines and triacylglycerols with total double bond number

Currently, the role of Notch signaling during myocardial infarction (MI) remains controversy. In this study we used in vitro and in vivo approaches to investigate the role of Notch signaling in MI. Using cultured human umbilical vein endothelial cells exposed to hypoxia/reoxygenation (H/R), we demonstrated that H/R inhibited the proliferation, VEGF secretion, and tube formation of HUVECs, and these effects were correlated with the inhibition of Notch signaling. Furthermore, these effects were antagonized by overexpression of NICD but aggravated by knockdown of NICD. In addition, in MI model rats we found that heart dysfunction and angiogenesis in model rats was partly improved by NICD overexpression but was aggravated by knockdown of NICD. In conclusion, these data demonstrate that Notch signaling is downregulated in H/R injury in the hearts. Artificial activation of Notch signaling could promote myocardial survival and angiogenesis and improve cardiac function following H/R injury.

Lung cancer is the leading cause of cancer-associated mortality worldwide. Elucidation of the pathogenesis and biology of lung cancer is critical for the design of an effective treatment for patients. Non-small cell lung cancer (NSCLC) accounts for 80-85% of lung cancer cases. The abnormal expression of Notch signaling pathway members is a relatively frequent event in NSCLC. The Notch signaling pathway serves important roles in cell fate determination, proliferation, differentiation and apoptosis. Increasing evidence supports the association of Notch signaling dysregulation with various types of malignant tumor, including NSCLC. Several studies have demonstrated that members of the Notch signaling pathway may be potential biomarkers for predicting the progression and prognosis of patients with NSCLC. Furthermore, Notch signaling serves critical roles in the tumorigenesis and treatment resistance of NSCLC cells by promoting the proliferation or inhibiting the apoptosis of NSCLC cells. The present review provides a detailed summary of the roles of Notch signaling in NSCLC.

Tou price is based on the price as the guidance, guiding power users according to their own electricity utilization way of flexibility and interest adjustment behavior to realize the purpose of peak peel and improve the load rate. This paper is on the basis of considering the user satisfaction, using plastic load capacity and power generation side coal saving benefit after two measures of implementing peak valley price demand response potential quantitative analysis, and feasibility of implementing peak valley price for a specific region. At last, a big industrial user in a certain area as an example for analysis, the result shows that the implementation of peak valley price can bring potential benefits, and provide a guide that the feasibility of implementing peak valley price in the region.

With the rise of micro-grid, renewable energy such as wind and solar power is introduced to the electric power grid, which has the random and intermittent characteristics, thus having great impact on the safety and stability for the electric power grid. This paper deeply analyzes the situation where multiple micro-grids are connected to power grid and establishes a scheme of joint scheduling model for micro-grids and distribution network, which considers network loss, load fluctuation and operation economy. Then a combined bi-level optimization algorithm is introduced to solve this model. The simulation result shows that reasonable scheduling of micro-grid and distribution network not only can smooth the load curve but also reduce network loss and achieve load shifting result.

High penetration of electric vehicles has brought huge impact on the safe and stable operation of power grid. How to effectively improve the comprehensive utilization rate of electric vehicle charging and discharging has become the most important problem for the grid operation. In this paper, we establish the centralized station model which takes the minimal real power loss as its objective and bring variance optimization function. Using IEEE 30-bus system as simulation system, this paper uses genetic algorithm to find the best solution of the above model. The result shows that reasonable arrangement of charge and discharge plans not only smoothes the load curve, but reduces real power loss.

In the coordination development course between wind power and power grid, because of flexible operation ability, the combined cycle gas turbine (CCGT) can strength the inherent unit commitment performance such as reserve security and ramp limit with the result of improving the power grids wind power absorption capacity. Thus, CCGT included wind power system is one way of sustainable development for civil energy and environment. Because the price of gas is much more expensive than that of coal, taking aspects of economy, technique and environment into account, this paper sets up comprehensive risk evaluation for combined cycle gas turbine involved wind power system, which includes index such as system operation cost, environmental benefit, unit capacity utilization rate, heavy ramping rate lasting time, wind power utilization rate, et al. The exact index calculation method is focused on. The case study shows that the large volume of wind power could spoil the trend of system evaluation index and introducing CCGT could enhance the economy and reliability of power system and thus has superior practical value for grid-source coordination with large volume of wind power injection and refined management of generation schedule.

Firstly, taking into account the load forecasting error and uncertainty of wind power, the coal saving benefit and network loss of the reserve are combined into integrated coal consumption of thermal units. Then, the consistency constraints of reserve capacity are derived. On the basis of the work mentioned above, the optimal reserve distribution model for power system with large scale wind power integration is proposed. This model is used to compare the network loss, integrated coal consumption and the allocation of reserve before and after adding consistency constraints. Finally, simulation results on the IEEE-RTS24 system verify the effectiveness of the algorithm in solving reserve transfer between units, ensuring the fairness of market and providing a reference to dispatchers.

An optimal assignment algorithm of emergency power supply considering V2G is proposed with a necessary concern for its effect on society. Firstly, the comprehensive index weight coefficients are proposed according to the priority. Next, this paper establishes the network minimum total cost objective function based on constraints such as time and capacity limit, using symmetric triangular fuzzy numbers to simulate the uncertainties in emergency logistics on the condition that ensuring the normal work of EV in next day which is used to search for the best logistics path. Finally the optimal algorithm of emergency power supply is developed based on genetic algorithm. The model and algorithm are proved by a numerical example, and the study helps to reduce the investment and operating cost of power system and improve the ability of urban electric network in emergency situation.

Based on the characteristics of current China's electricity power market, it is necessary to develop the self-regulation system for all market participants. This paper sets up index system based comprehensive risk evaluation for self-regulation construction, which covers market infrastructure stability, schedule fairness,trade compliance, electricity bill settlement risk. The evaluation criterion for each indicator is given afterwards. The key self-regulation information and subsequent trade behavior improvement measures can be attained after making the evaluation by calculating each index value, thus conducive to achieving the power market transaction stability and efficiency.

In the emergency management of power system, there are many uncertain factors, such as wind power of the generation side and electric vehicle charging of the demand side. Firstly, the probability statistics model of PEVs charging demands was established, which considered the random factors of the probability distribution of PEVs charging. Then, we used scenarios generation and reduction techniques to predict the output of wind generators. The uncertainty of wind power was described by scenarios set. On this basis, the dispatch model considering PEVs charging and wind power was proposed. Finally, simulation results on the 5-generator system indicated the effectiveness of the proposed method. The synergistic dispatch model considering wind power and PEVs charging can effectively improve the utilization of wind power and the safety and economic benefits of the power system.

Background Coronary artery bypass grafting is employed for the treatment of multiple-vessel lesions. Studies have shown that continued maladaptive remodelling expedites atherosclerosis and that excessive remodelling leads to graft restenosis. However, few studies have focussed on mechanistic target of rapamycin (mTOR) signalling involved during remodelling of transplanted veins. Methods Rabbits were randomly allocated to groups undergoing vein-graft restenosis or sham surgery. At 1, 3, 7, 14, 28 and 90 days after surgery, we removed bypassed grafts and placed them in groups named T 1 , T 2 , T 3 , T 4 , T 5 and T 6 , respectively. Group T 0 denoted the control group. Analysis included vessel thickness, electron microscope data, TUNEL staining, and expression of the proliferation-associated gene proliferating cell nuclear antigen (PCNA). We chose specific time-points of gene expression, and then observed changes in the mTOR signalling. Results The early stage of vein grafting (one to three days after surgery) led to apoptosis and degradation of the extracellular matrix. Seven days after surgery, cells began to proliferate. RICTOR expression in mTOR complex 2 (mTORC2) and that of its downstream substrate protein kinase C was enhanced in the early stage (T 1 and T 3 ), and was higher in T 1 . mTORC1's upstream gene regulation decreased in T 1 and increased in T 3 . Its downstream genes eIF4b and 4E-BP showed similar changes. Expression of eIF4e and eIF4b increased, and led to an increase in protein composition. Conclusions After transplantation, mTORC1 function and its upstream and downstream genes were inhibited on the first day after grafting, but mTORC2 function was enhanced. One week after surgery, mTORC2 was still overexpressed when mTORC1 function had recovered and became enhanced. Hence, mTORC2 plays a major role in arterialisation of veins.

Turbo-generator stator's inter-turn short is a usual serious fault, there would have hidden big trouble for electric power system's safety due to lack of efficient protection. On-line monitoring generator's operate condition combined intelligence non-line identify technology is presented to observe fault in time instead of poor function of protection. Longitudinal zero-sequence voltage and fault phase's current are analysis as stator winding's inter-turn short's stable fault characters, mathematical model of which are build, Elman neural network which do well for dynamic data in real time are introduced to identify the fault. A large turbo-generator's general parameters are used for calculate its stable fault characters during stator winding's inter-turn short occur in operation, and identification are performed by trained Elman neural network followed. Example indicate that the Elman network could efficiently identify generator stator's inter-turn short based on rational fault characters combine.

Enteral feeding can be given either through the nasogastric or the nasojejunal route. Studies have shown that nasojejunal tube placement is cumbersome and that nasogastric feeding is an effective means of providing enteral nutrition. However, the concern that nasogastric feeding increases the chance of aspiration pneumonitis and exacerbates acute pancreatitis by stimulating pancreatic secretion has prevented it being established as a standard of care. We aimed to evaluate the differences in safety and tolerance between nasogastric and nasojejunal feeding by assessing the impact of the two approaches on the incidence of mortality, tracheal aspiration, diarrhea, exacerbation of pain, and meeting the energy balance in patients with severe acute pancreatitis. We searched the electronic databases of the Cochrane Central Register of Controlled Trials, PubMed, and EMBASE. We included prospective randomized controlled trials comparing nasogastric and nasojejunal feeding in patients with predicted severe acute pancreatitis. Two reviewers assessed the quality of each study and collected data independently. Disagreements were resolved by discussion among the two reviewers and any of the other authors of the paper. We performed a meta-analysis and reported summary estimates of outcomes as Risk Ratio (RR) with 95% confidence intervals (CIs). We included three randomized controlled trials involving a total of 157 patients. The demographics of the patients in the nasogastric and nasojejunal feeding groups were comparable. There were no significant differences in the incidence of mortality (RR = 0.69, 95% CI: 0.37 to 1.29, P = 0.25); tracheal aspiration (RR = 0.46, 95% CI: 0.14 to 1.53, P = 0.20); diarrhea (RR = 1.43, 95% CI: 0.59 to 3.45, P = 0.43); exacerbation of pain (RR = 0.94, 95% CI: 0.32 to 2.70, P = 0.90); and meeting energy balance (RR = 1.00, 95% CI: 0.92 to 1.09, P = 0.97) between the two groups. Nasogastric feeding was not inferior to nasojejunal feeding. Nasogastric feeding is safe and well tolerated compared with nasojejunal feeding. Study limitations included a small total sample size among others. More high-quality large-scale randomized controlled trials are needed to validate the use of nasogastric feeding instead of nasojejunal feeding.