1. Chronic intracerebroventricular infusion of lipopolysaccharide: effects of ibuprofen treatment and behavioural and histopathological correlates
- Author
-
Tom Gardiner, R.L. Richardson, Eugene O'Hare, and Eun-Mee Kim
- Subjects
Lipopolysaccharides ,Male ,Reinforcement Schedule ,Lipopolysaccharide ,Ibuprofen ,Inflammation ,Pharmacology ,chemistry.chemical_compound ,Memory ,Glial Fibrillary Acidic Protein ,Animals ,Medicine ,Antigens ,Rats, Wistar ,Injections, Intraventricular ,Amyloid beta-Peptides ,Behavior, Animal ,Microglia ,business.industry ,Anti-Inflammatory Agents, Non-Steroidal ,Brain ,Neurofibrillary Tangles ,Immunohistochemistry ,Cannula ,Rats ,Psychiatry and Mental health ,medicine.anatomical_structure ,chemistry ,Food ,Ventricle ,Astrocytes ,Anesthesia ,medicine.symptom ,business ,Reinforcement, Psychology ,Artificial cerebrospinal fluid ,medicine.drug - Abstract
Twenty male Wistar rats were trained under an alternating-lever cyclic-ratio (ALCR) schedule of food reinforcement. When responding showed no trends, each subject was subcutaneously implanted with an Alzet osmotic mini-pump, connected to a chronic indwelling cannula extending into the lateral ventricle of the brain. The mini-pumps were primed to infuse 0.25 microl lipopolysaccharide (LPS) (1.0 microg/0.25 ml) or 0.25 microl artificial cerebrospinal fluid (aCSF) per hour and were implanted for 28 days. LPS infusion produced behavioural deficits which chronic ibuprofen treatment (40 mg/kg every 12 h) alleviated. Infusion of LPS induced R 1282-positive amyloid deposits, and activation of microglia and astrocytes. Ibuprofen treatment reduced the numbers of activated microglia, and withdrawal of ibuprofen resulted in an increase in activated microglia; however, ibuprofen treatment had no effect on numbers of activated astrocytes in the LPS-infused subjects.
- Published
- 2005