1. Pertussis toxin–induced inflammatory response exacerbates intracerebral haemorrhage and ischaemic stroke in mice
- Author
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Ming Zou, Junwan Fan, Jingli Cao, Ying Zhang, Yuwhen Xiu, Wei-Na Jin, Yan Feng, Wenyan He, Haowen Li, and Yan Li
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,medicine.medical_treatment ,Inflammation ,Brain damage ,Pertussis toxin ,Systemic inflammation ,Brain Ischemia ,Lesion ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Animals ,Medicine ,cardiovascular diseases ,RC346-429 ,Stroke ,Cerebral Hemorrhage ,Ischemic Stroke ,business.industry ,medicine.disease ,030104 developmental biology ,Cytokine ,Pertussis Toxin ,Neurology. Diseases of the nervous system ,Neurology (clinical) ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,030217 neurology & neurosurgery - Abstract
BackgroundStroke is a devastating disease, including intracerebral haemorrhage (ICH) and ischaemic stroke. Emerging evidences indicate that systemic inflammatory cascades after stroke contribute to brain damage. However, the direct effects and features of systemic inflammation on brain injury, especially comparing between ischaemic and haemorrhagic stroke, are still obscure.MethodsPertussis toxin (PT) was used to build a pro-inflammatory milieu after ICH and ischaemic stroke in mouse model. The neurodeficits, stroke lesion, immune response and blood–brain barrier (BBB) destruction were assessed.ResultsIn ICH mouse model, PT-induced systemic inflammation exacerbated neurological deficits, and enlarged haemorrhage lesion and perihaematomal oedema. We also found promoted leucocyte infiltration and inflammatory cytokine release into the brain after PT treatment. Moreover, the integrity of the BBB was further disrupted after receiving PT. Furthermore, we demonstrated that PT enhanced brain inflammation and aggravated stroke severity in middle cerebral artery occlusion mouse model.ConclusionsOur results suggest that PT increases inflammatory response that exacerbates brain injury after ICH or ischaemic stroke in mouse model.
- Published
- 2021