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The expression of human transfer ribonucleic acid (tRNA) methyltransferase 9-like (KIAA1456) protein in lung cancer tissue and its effects on certain genes involved in the proliferation, migration and invasion of lung cancer cells were investigated. Immunohistochemistry was applied to stain lung cancer tissue and adjacent tissue sections of 90 lung cancer patients, so as to evaluate the difference in the expression level of KIAA1456 between two tissues. The correlation of KIAA1456 expression with clinicopathological parameters of lung cancer was analyzed. Kaplan-Meier survival curves were used to analyze the relationship between KIAA1456 and postoperative survival in patients with lung cancer. KIAA1456 gene was overexpressed in lung cancer cell lines (A549 and GLC-15), and the influence of KIAA1456 gene on the expression of cyclin D1, neural cadherin (N-cadherin) and epithelial cadherin (E-cadherin) and their involvement in lung cancer cell proliferation, migration and invasion were observed. Compared with that in adjacent tissue, the expression of KIAA1456 in lung cancer tissue was significantly decreased (p

The aim of the present study was to determine the prevalence and related drug resistance of AmpC β-lactamases in Acinetobacter baumannii in tertiary-level hospitals in the Xuzhou region in China. A total of 134 clinical isolates of non-repetitive Acinetobacter baumannii were collected from different hospitals in the Xuzhou region, and multiplex polymerase chain reaction (PCR) was employed to determine the genotype of AmpC. The PCR products were purified and sequenced. The susceptibility to antibiotics was tested using the biometrics automated microbiological-assay system, VITEK-2. Amongst the 134 isolated strains, 96 strains were found to produce AmpC β-lactamases, and the positive rate was 72%, all of which carried acinetobacter-derived cephalosporinase (ADC) type AmpC resistance genes. The drug sensitivity tests indicated that the positive Acinetobacter baumannii strains were resistant to the majority of extended-spectrum β-lactam antibiotics, but were only sensitive to polymyxin. In conclusion, the incidence of AmpC enzymes in Acinetobacter baumannii strains in tertiary-level hospitals in the Xuzhou area is relatively high, and resistance to the majority of extended-spectrum β-lactam antibiotics may be related to the ADC type of AmpC.

Several studies have reported a significant role of high mobility group box protein 1 (HMGB1) in lung cancer. Nevertheless, there is a lack of knowledge regarding the expression of HMGB1 and its correlation with the clinicopathological features of lung cancer. In addition, the potential molecular mechanisms underlying the role of HMGB1 in lung cancer are still unknown. We therefore investigated the clinicopathological and prognostic significance as well as the potential role of HMGB1 in the development and progression of lung cancer. HMGB1 expression in the tumor tissues of the cohort correlated with clinicopathological features. Moreover, lung cell migration and invasion were significantly increased after treatment with HMGB1. The matrix metalloproteinase-2 (MMP-2) expression and activity were upregulated after treatment with HMGB1, while the upregulated expression of MMP-2 stimulated by HMGB1 in lung cancer cells was significantly reduced with the blockage of si-p65. These results indicated that HMGB1 expression was significantly associated with lung cancer progression. We also showed that HMGB1 promoted lung cancer invasion and metastasis by upregulating the expression and activity of MMP-2 in an NF-κB-dependent manner. Taken together, these data suggested that HMGB1 may be a potential prognosis and therapeutic marker for lung cancer.

Background There have been few investigations conducted on the epidemiology and risk factors of osteoporosis in patients with diabetes in China. The aim of this study was to disclose the percentage and the possible risk factors that influence bone mineral density (BMD) in this population. Methods One hundred thirty-one elderly male patients with type 2 diabetes (73.12 ± 5.54 years of age, diabetic duration 5.28 ± 3.56 years, HbA 1C 7.87% ± 1.82 %), >65 years of age were recruited. All subjects were submitted to dual-energy X-ray absorptiometry to evaluate the BMD at lumbar spine (LS), femoral neck (FN), and femoral trochanter (FT). Fasting blood samples were taken to check hormones and biochemical levels. Demographic data were also collected. Results In the different bone sites studied, the percentage of osteoporosis and osteopenia varied in this group of patients ranging from 29.0 to 31.3% and 29.0 to 33.6%, respectively. Weight, HbA 1C , body mass index, and testosterone were correlated with BMD at all the bone sites studied. Weight was the best predictor among the above parameters for BMD by linear regression analysis at LS and FN, whereas testosterone was the best predictor for BMD at FT. Conclusions In the different bone sites studied, the percentage of osteoporosis and osteopenia varied ranging from 29.0 to 31.3% and 29.0 to 33.6%, respectively. Weight was the best predictor for BMD at LS and FN and testosterone was the best predictor for BMD at FT. Poor glycemic control was correlated with osteoporosis.

Norcantharidin has been used as an efficacious anticancer drug in China for many years, but its true mechanism remains poorly understood. Intriguingly, in an in vitro series study of anticancer drugs, we found that norcantharidin can effectively inhibit epithelial tumor cells from expressing integrin alphavbeta6. Our previous studies have confirmed that integrin alphavbeta6 is closely relevant to malignant epithelial cell tumor biology behavior, and it can promote cancer cells to invade and metastasize through a special alphavbeta6-extracellular signal-related kinase (ERK) direct signaling pathway. In this study, we investigated the relationship between the norcantharidin anticancer mechanism and integrin alphavbeta6. After HT-29 colon cancer cells were treated with norcantharidin, cell apoptosis increased remarkably. The expression of alphavbeta6 and the amount of p-ERK decreased substantially; simultaneously, the linkage between alphavbeta6 and ERK was barely detectable. However, the expression of other integrins and the levels of mitogen-activated protein kinase hardly changed. On these grounds, we presumed that norcantharidin induced HT-29 colon cancer cell apoptosis through the alphavbeta6-ERK signaling pathway. This finding elicited a novel strategy for targeting the whole alphavbeta6-ERK signal pathway, rather than simply blocking the combining site of alphavbeta6-ERK in colon cancer treatment.

Multi-carrier (MC) multi-user CDMA is a new transmission method in which every user bit is sent simultaneously over multiple subcarriers (or multiple tones), each of which has a time-constant 0 or /spl pi/ phase offset. The offsets corresponding to each user form a unique code signature sequence, orthogonal to those of any other user, or pseudo orthogonal as with Gold codes or Kessami code sequences. We address a moderately dispersive channel in which each user-subcarrier channel has a different random amplitude and phase. We estimate the performance of a receiver matched to such a multicarrier, multi-user scheme. Then we implement a decorrelating interference canceler at the output and examine its effect on performance.