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Self attention mechanism is widely used in relation extraction, emotion classification and other tasks. It can extract a wide range of relevance information in the text. The attention mode of the existing self attention mechanism is soft attention mode, that is, a dense attention matrix is generated by softmax function. However, if the sentence length is long, the weight of important information will be too small. At the same time, the softmax function assumes that all elements have a positive impact on the results by default, which makes the model unable to extract the negative effect information. We use hard attention mechanism, namely sparse attention matrix, to improve the existing self attention model and fully extract the positive and negative information of text. Our model can not only enhance the extraction of positive information, but also makes up for the blank that the traditional attention matrix cannot be negative. We evaluated our model in three tasks and seven data sets. The experimental results show that our model is superior to the traditional self attention model and superior to state-of-the-art models in some tasks.

Background: Jingui Shenqi （JGSQ for short）pills is a traditional Chinese medicine formula, which has the functions of warming and tonifying kidney-yang, generating essence and filling marrow, warming tendons and veins and bones, and improving bone mineral density. The aim of this study was to investigate the effect and mechanism of JGSQ pills in preventing and treating postmenopausal osteoporosis.Methods : Twelve-week-old SPF female SD rats (n=48) were used in this study. Following the ovariectomy operation (n=40), the rats were randomly divided into the model group, (high, medium, and low dose groups) treated with Jingui Shenqi pills and estradiol group. Recorded the weight gain of rats and calculated the uterine coefficient; To detect the expression of serum calcium, phosphorus, ALP and OPG; HE staining was used to detect the pathological changes of femur; In all the groups, Micro-CT was used for detection of bone mineral density and bone microstructure; and gene expression of BMP-2, Smad1, and Runx2 in rat bone tissue was determined by RT-PCR and Western Blot methods.Results: Compared with the sham operation group, rats in the model group had the highest increase in body weight and the lowest uterine coefficient. Each of the treatment groups had a modest increase in weight gain. Micro-CT and HE staining showed a decrease in bone mineral density in the model group with shorter, thinner, broken, and osteoporotic trabeculae of the femur. The bone mineral density in Jingui Shenqi pills treatment groups had a significant increase with an improved bone microstructure and intact bone trabecular as compared to the model group. Serum ALP in the model group was significantly higher than in the sham operation group but the serum calcium, phosphorus and OPG was significantly lower. The Jingui Shenqi pills treatment groups and the estradiol treated group had lower serum ALP and increased serum calcium, phosphorus and OPG.There was decreased gene expression of BMP-2, Smad1, and Runx2 in the bone tissue of the model group compared to the sham operation group. BMP-2, Smad1, and Runx2 gene expression in Jingui Shenqi pills treated group and estradiol treated group was significantly higher than that of the model group.Conclusion: Jingui Shenqi pills improve the microstructure of bone tissue and increase bone mineral density thus resolving osteoporosis. This is achieved by regulating BMP/Smad signaling pathway and increasing the expression of osteogenic factors BMP-2, Smad1, and Runx2.

Ischaemic stroke (IS) is a common type of stroke characterised by sudden fainting and communication disorders, alongside a number of other symptoms. It is characterised by high morbidity, disability, and mortality rates. Tongqiao Huoxue Decoction (THD) is effective in the treatment of stroke. As a representative prescription for promoting blood circulation and removing blood stasis, THD has been widely used clinically. This paper systematically introduces clinical and experimental studies of THD in the treatment of IS, summarising its clinical application, pharmacological mechanisms, and active components in the treatment of IS. It also explores its key pathways in the treatment of IS through network pharmacology analyses, thereby speculating on its underlying mechanisms. It is of great significance for the secondary development of this classic prescription as well as for the research and development of new drugs.

Background: Esophageal diverticulum (ED) is an extremely rare complication of congenital esophageal atresia (EA) with or without tracheoesophageal fistula (TEF) surgery. We aimed to investigate feasible methods for the treatment of this rare complication.Methods: We retrospectively reviewed all patients with EA/TEF at Beijing Children's Hospital from January 2015 to September 2019. The clinicopathological features of patients with ED after EA/TEF surgery were recorded. Follow-up was routinely performed after surgery until December 2020.Results: Among 198 patients with EA/TEF, ED only occurred in four patients (2.02%; one male, three female). The four patients had varying complications after the initial operation, including anastomotic leakage (3/4), esophageal stenosis (3/4), and recurrence of TEF (1/4). The main clinical symptoms of ED included recurrent pneumonia (4/4), coughing (4/4), and dysphagia (3/4). All ED cases occurred near the esophageal anastomosis. Patients' age at the time of diverticulum repair was 6.6–16.8 months. All patients underwent thoracoscopic esophageal diverticulectomy (operation time: 1.5–3.5 h). Anastomotic leakage occurred in one patient and spontaneously healed after 2 weeks. The other three patients had no peri-operative complications. All patients were routinely followed up after surgery for 14–36 months. During the follow-up period, all patients could eat orally, had good growth and weight gain, and showed no ED recurrence or anastomotic leakage on esophagogram.Conclusions: ED is a rare complication after EA/TEF surgery and is a clear indication for diverticulectomy. During the midterm follow-up, thoracoscopic esophageal diverticulectomy was safe and effective for ED after EA/TEF surgery.

Thermochemical heat storage (THS) systems have major advantages over other thermal storage systems, notably high energy density and low heat loss when hermetically sealed. There are several review papers available that discuss THS. Unlike other published review articles, this paper presents a literature survey and a review that add insights into the current state-of-the-art THS technologies, covering: the THS materials, THS reactor design and THS as thermal batteries. Emphasis is placed on THS for solar thermal energy storage and also for industrial waste heat recovery. At the materials level, in addition to a review on THS material sorbents, emphasis is placed on innovative composite THS materials with salt mixtures and metal-organic frameworks materials. Reactor design is one of the major fields of THS system development. In this paper, we also review several types of innovative reactor designs, including hybrid THS systems, towards obtaining advanced reactor concept, numerical studies in THS studies mainly covering the heat and mass transfer in the reactor designs, and also the implementation of THS systems as thermal batteries. Among the main conclusions, it is found that, although several advancements have been achieved in these fields in the last decade, further research is needed for advancing THS technology to be commercially viable. This paper will provide a wide range of information including the research gaps and critical issues in this field. The authors aim to allow readers to identify gaps/issues in the current research towards improving the practicality of THS systems.

In view of the problem that it is difficult to supervise and improve the attention level of teenagers in class in education and teaching activities, based on multi-source data correlation, we combine with big data technology to analyze and research the attention level of teenagers in class. This paper analyzes the collected eye characteristics data of students in the classroom and other personalized related data to conduct a correlation analysis. Then, we derive a set of theoretical models to remind students of concentration improvement, as well as intervention of teachers’ teaching activities. Finally, the proposed theoretical model can improve the teaching quality ultimately.

IntroductionPrevious research had shown that the ethyl acetate extract from Wenxia Formula (WFEA extract) had significant effects in inhibiting tumor and cancer associated fibroblasts (CAFs). However, the anti-cancer mechanism of the WFEA extract was still unclear. In this study, we aimed to illuminate the possible anti-cancer mechanisms of the WFEA extract.Material and methodsUltra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) was applied to analyze and identify the constituents of WFEA extract. The lung cancer xenograft model was conducted to explore the antitumor activity of WFEA extract by measuring tumor volume and weight, HE staining, immunohistochemistry, and immunofluorescence assay. Western blot and qRT-PCR were used to measure the expressions of related proteins and genes in the Hedgehog- Gli1 (Hh-Gli1) signaling pathway.ResultsA total of 15 chemical constituents were identified in the WFEA extract. The xenograft model showed that WFEA extract could inhibit tumor growth and had no significant effect on body weight of mice. In addition, WFEA extract reduced the expression of a-SMA, FAP and N-cadherin in the CAF mediated lung cancer xenograft model and increased the expression of E-cadherin. Western blot and qRT-PCR showed that WFEA extract reduced the expression of related proteins and genes (Smo, Gli1 and Ptch1) in the Hh-Gli1 signaling pathway.ConclusionsWFEA extract had a certain inhibitory effect on lung cancer, and its mechanism may be related to the CAF mediated Hh-Gli1 signaling pathway.

Background Neurological deficits is one of the most prevalent clinical manifestation after stroke. The effects of astrocytes activated by electroacupunture (EA) after stroke on the neurological recovery in middle cerebral artery occlusion (MCAO) rats was not clear and definite. Objective Our previous study showed that treatment with EA for 7 days contributed to the activation of astrocytes in MCAO rats. The purposes of this study were to 1) confirm the effects of EA for 14 days on activation of astrocytes in MCAO rats, and 2) test the relationships between activation of astrocytes and neurological functional recovery induced by EA in MCAO rats. Methods All rats were randomly divided into five groups: naive control group, sham control control group, MCAO, MCAO/EAn, MCAO/EAd (n = 8, for each group). Rats in MCAO/EAn group received EA treatment at acupoints of Neiguan (PC06). MCAO/EAd group received EA stimulus at acupoints of Diji (SP08). The primary indicators were locomotor recovery, histopathology, immunohistochemistry, RT-PCR and Western blot. Results The neurological deficit and histopathological improvements and activation of astrocytes were observed after EA treatment at acupoints PC06. Parametric correlation analyses revealed a cubic correlation relationship between activation of astrocytes and neurological recovery of MCAO rats treated with EA. Conclusion EA treatment at the acupoints of Neiguan involved in the regulation of activation of astrocytes, which our data suggested has a cubic correlation relationship with the neurological recovery of MCAO rats.

The hybrid MEMS diffuser device based on Si wafer and fire resistant 4 printed circuit board (FR-4 PCB) was designed in order to obtain a low-cost device with large aperture for laser speckle reduction. Reflective diffuser with continuous height profile was fabricated on Si wafer by micromachining technology. FR-4 PCB was used to fabricate the vibrating structure by printed circuit board (PCB) manufacturing process. The hybrid MEMS diffuser device including diffuser and vibrating structure was driven to vibrate by electromagnetic force with a frequency of 66 Hz. The laser speckle reduction of 71.8% was obtained for the MEMS diffuser device.

The economic efficiency of small-scale tilapia farms in Guangxi, China were analyzed by using Data Envelopment Analysis (DEA). The mean technical (under CRS and VRS), allocative, and economic efficiencies of small-scale tilapia farms were 0.89, 0.97, 0.71 and 0.68, respectively. Efficiency analysis revealed that inefficient farms would have needed to increase production output levels by 32% to perform as well as the best practice farms. The decomposition of the economic efficiency showed that allocative inefficiency was the primary cause of economic inefficiency. There were positive relationships between economic efficiency and age, culture mode and period through Tobit regression, while experience, family members, and technology support had negative effects on economic efficiency. Lower input levels of feed quantity may help to increase the tilapia farming economic efficiency.

A zero-order resonant antenna based on composite left and right hand (CRLH) transmission line and reactive impedance surface (RIS) is presented. The present antenna, which has a single-fed configuration, is loaded with the CRLH and RIS for miniaturization, dual-band and increase bandwidth purpose. It is a three-layer structures with a dielectric constant of 4.02 for the upper and lower substrates. The RIS, which is composed of an 8 by 8 periodic arrays of metallic square patch printed on a grounded dielectric substrate, is introduced below the top surface. This antenna exhibits an overall size is $\mathbf{0.037\lambda 0\times 0.025 \lambda_0}$ at 8.48 GHz. The simulated 10dB return loss bandwidths are 18.9% at 8.48GHz and 2.4% at 12.36 GHz respectively. The gain values are 6.59 dBi at 8.48 GHz and 5.26 dBi at 12.36 GHz.

In the treatment of lung cancer, the multidrug resistance to chemotherapeutic drugs is one of the reasons of low rates for cure and treatment failure, the combination of chemotherapeutic drugs and traditional Chinese medicine can increase the sensitivity of chemotherapy and reduce its adverse effects. Our previous study has proved that Chinese herbal medicine (CHM) Wenxia Changfu Formula (WCF for short) effectively enhances chemotherapeutic efficacy in lung cancer treatment and reverses multidrug resistance in lung cancer cells in vitro. The present study aims to investigate the effect and mechanism of WCF in reversing cell adhesion-mediated drug resistance of lung cancer by using A549 three-dimensional cell culture and nude mouse model of the A549 cell line with Integrin β1 overexpression. We show that the combination of WCF with DDP can decrease proliferation of lung cancer cells by inducing cell cycle arrest and apoptosis. Moreover, we find that the combination of WCF with DDP suppresses the expression of certain molecules which regulate cell cycle and apoptosis. Mechanistically, we show that the Integrin β1, FAK, PI3K, and AKT protein expressions are suppressed by DDP and even more responses are observed when DDP and WCF are combined, showing WCF treatment enhances the effect of commonly used anticancer drugs. In line with the above findings, our results confirm that WCF reverses cell adhesion-mediated drug resistance of lung cancer via inactivating Integrin β1/PI3K/AKT and apoptosis induction.

Based on the 1MWt parabolic trough concentrating solar power (CSP) pilot plant, the operation characteristics are investigated in three typical weather conditions around the vernal equinox. A simplified performance prediction model is proposed, and the simulation results are compared with the measured data. The results show that the change of the heat transfer oil temperature at the loop inlet will be transferred to the loop outlet after a while. The fluctuation of direct normal irradiance (DNI) has little effect on the outlet temperature. The model is able to accurately predict the performance of the parabolic trough systems at stable flow rate and stable inlet temperature. Installation accuracy of parabolic trough collectors as well as the cleanliness of mirrors has a great influence on the collectors’ solar-to-thermal efficiency, therefore, high standard of installation and maintenance is necessary.

The aim of this study is to investigate the mechanisms of Chinese herbal medicine called “Baoyuan Jiedu” (BYJD for short) decoction, improving life quality and preventing muscle atrophy of cancer cachexia model mice. We showed that the effect of BYJD decoction increased body weights of mice and reduced tumor volume and tumor mass. Furthermore, BYJD decoction increased the gastrocnemii mass and the transverse diameter of muscle fiber morphology. Moreover, BYJD reduced the expression of Atrogin-1 and MuRF-1 protein. Collectively, our results show that BYJD decoction improves the life quality of cancer cachexia mice and prevents muscle atrophy by downregulating expression of Atrogin-1 and MuRF-1.

This paper discusses and compares some common architectures used in autonomous mobile robotics. Then it describes a behavior-based autonomous mobile robot that was implemented successfully in the Robotics Lab of the Department of Precision Mechanical Engineering. Fuzzy controller was used to implement the emergency behavior, the behaviors arbitration was implemented using the subsumption architecture. In an unknown dynamic indoor environment, the robot achieved real-time obstacle avoidance properties that are cruel for mobile robotics.

A Laser Diode(LD) end-pumped Nd:YLF quasi-continuous 351 nm ultraviolet laser with high efficiency and high peak power was demonstrated under acousto-optical Q-switched operation, by using extracavity frequency-doubled and frequency-tripled with two LiB3O5(LBO) crystals cling to the output mirror. An average output power at 351 nm of 450 mW was obtained at repetition rate of 1 kHz, pumped power of 14 W and output power of fundamental wave of 1.45 W. The optical-to-optical conversion efficiency is up to 31.04% and pulse width is 7.5 ns corresponding to the peak power as high as 60 kW.The beam quality is satisfactory.

The micro robot based on screw motion wheels, which features high payload/mass ratio, fast and continuous motion, adaptation to pipe diameter or roundness variations, is suitable for locomotion and inspection inside small-diameter pipelines. The robot inspection system, Tubot I, developed at Shanghai University is composed of locomotion mechanism with an inner motor, a micro CCD camera and a monitor outside the pipeline. In the paper, the kinematics and statics analyses are presented for the screw locomotion system of Tubot I. The moving characteristics are obtained from experiments on the robot prototype.

Experiment was carried out using Ziyuan III (ZY-3 for short) stereo images in order to extract DEM and corresponding DEM precision analysis was performed. The DEM was built by using ZY-3 stereo images on ENVI platform, then taking 1:10000 scale DEM as reference data, the extracted DEM and reference DEM were processed in ArcGIS10 platform, such as re-sampling and projection conversion and so on. The extracted DEM elevation accuracy was evaluated effectively by calculating the elevation error and average error indicators. The result shows that the DEM extraction from stereo image of ZY-3 has good feasibility based on ENVI software and the extracted DEM in the hilly and gully region height accuracy can reach 35m with no control points.

In ICF (Inertia confinement fusion) physical experiments, target-positioning accuracy directly affects the success rate of target hitting. This study uses three CCDs to acquire images of target from different viewing angles then the spatial coordinate values and the rotation matrix of target are calculated by integrating the characteristic values of the three CCDs. To obtain the characteristic values, this paper presents an image processing method of data fusion which combines template matching with ellipse contour fitting technology. Sub-pixel accuracy of location is obtained by adopting bicubic interpolation. Proved by practice test this method has the advantages of high stability, low time consumption and high accuracy over 1 μm.

As for some possible risk factors in the supply chain operation, the system framework model based on Multi-agent is put forward and we try to build the simulation experiment platform of supply chain operation risk management. By simulation, many real situations can be realized, some virtual but more typical data can be collected, then we can analysis on the basis of these typical results, and explore the occurrence of risks and responding strategies.

China is furthering its reform of state-owned enterprises and establishing a modern enterprise system. Human resource as a important part of the modern enterprise system. Job analysis sits at the heart of all human resource practices, making it a critically important in every organization. However, the central enterprises’ special role in national economy determined the job analysis for the central enterprises’ executive position must follow certain rules. In this paper, we discussed Job analysis model and implementation strategy for the central enterprises’ executive position.

This paper presents a method of using nonlinear decision function to improve the performance of AdaBoost with SVM based weak learners. Compared with the existing AdaBoostSVM methods,this method, named ERBF-AdaBoostSVM, has advantages of higher hate rate and better generalization performance. This method also provides non-linear separator in the weak learner space and classifies accurately more examples. Experimental results demonstrated that ERBF-AdaBoostSVM achieve better generalization performance and higher hate rate than the existing SVM and AdaBoostSVM methods.

The process of software development, including documentation, design, program, test, and maintenance can be measured statistically. Therefore the quality of software can be monitored efficiently. Software metrics is very important in research of software engineering and it has developed gradually. In this paper, software metrics definition were given and the history of and the types of software metrics were overviewed. Software complexity measuring is the important constituent of software metrics and it is concerning the cost of software development and maintenance. In order to improve the software quality and the project controllability, it is necessary to control the software complexity by measuring the related aspects. This paper respectively expounds McCabe methods and C & K metric method for examples of complexity metrics

This paper is concerned with a control method of an exoskeletal ankle based on surface electromyographic (sEMG) signals. The sEMG signals of human ankle are introduced first. The muscles which are medial gastrocnemius(MG) and tibialis anterior(TA) are selected by experiments. Then a threshold method of controlling the exoskeletal ankle using sEMG signals is designed. A control system is realized. The sEMG signals are acquired, and sent to the computer. The computer deals with the sEMG signals and generates the control orders. The control orders are passed to the motor controller and drive the exoskeleton to move. At last, through experiment, the control method is verified and the effectiveness of the control system is proved.

This paper is concerned with studying human ankle movement based on surface electromyographic(sEMG) signals. The sEMG signals were collected from the lateral gastrocnemius(LA), medial gastrocnemius(MG), tibialis anterior(TA) and peroneus longus(PER) muscles of normal subjects when they moved their ankle flexion-extension. Four types of movement were designed including maximum voluntary contraction, bending/extending, going down, walking. The raw EMG signals were processed and analysis. The experimental results also showed that (1) The sEMG signals of TA and MG are profitable during movement of ankle joint, (2)There are some regularities of EMG signals in gait period. The characteristics of sEMG signals are favorable for using them for control purposes.

In this paper, a new navigation method for intelligent colonoscope robot is presented. The proposed navigation method is developed to facilitate colonoscope into a human colon. The navigation method is based on the sensory fusion utilizing the quantitative parameters from the captured images and the tactile sensors. In vision navigation method, dark region which is described as the direction of human colon can be segmented from colonoscope image after auto-thresholding algorithm. Auto-thresholding algorithm is based on Ostu's method and the method is optimized by analyzing the endoscope image. The tactile sensors used here is a bridge circuit of force sensitive resistors built on silicon base by MEMS technology. Four sensors are mounted symmetrically around the colonoscope's head, so they can measure the pressure force in almost all directions. The proposed navigation method was tested with animal colons and the experimental observations are discussed.

In this paper, the design of an intelligent endoscope navigation system is presented. The proposed intelligent endoscope system is a vision and tactile guided device, developed to facilitate navigation inside a human colon. The design of the entire system is divided into three parts viz. design of an automatic insertion system, a vision and tactile guided system, and a computer control system. The endoscope insertion system which has force sensors is explained. The navigation system is based on the sensory fusion utilizing the quantitative parameters from the captured images and the tactile sensors. An auto-thresholding algorithm is described for extracting the lumen from endoscopic images. A computer control system to control the directional movements of the endoscope is also explained. The proposed endoscope system was tested with animal colons and the experimental observations are discussed.

The paper studies a tactile sensor. This micro-semiconductor tactile sensor is made of silicon and can be used for the automatic obstacle-avoidance system of endoscope. This paper presents details of the principle of the micro silicon semiconductor sensor at first. Then its design is presented. The application of the sensor in intelligent endoscope system which uses MEMS technology is also explained. At last, the experiment results and analysis are presented.

Two novel kinds of slim FBG (fiber Bragg grating) sensor devices and their application are presented, which can show the three-dimensional image of colonoscope shape on a video screen. One of the two kinds of sensor device is for gradual detection; the other is for real-time dynamic reconstruction. The sensing network device comprises a plurality of FBG sensors mounted on the body of a flexible wire. After the colonoscope is advanced in the body, the wire is pushed through the biopsy channel of the colonoscope. It bends with the curvature of the colonoscope. The FBG sensors capture deformation of the sensor wire, and reconstruct the shape of colonoscope. Experiments in the colon of a live swine have been done. Configuration of experimental system and experimental result are also given.

Refereed/Peer-reviewed A multifunction, high-sensitivity, and temperature-compensated optical fiber DNA hybridization sensor combining surface plasmon resonance (SPR) and Mach–Zehnder interference (MZI) has been designed and implemented. We demonstrate, for the first time to our knowledge, the dual-parameter measurement of temperature and refractive index (RI) by simultaneously using SPR and MZI in a simple single-mode fiber (SMF)–no-core fiber (NCF)–SMF structure. The experimental results show RI sensitivities of 930 and 1899 nm/RIU and temperature sensitivities of 0.4 and −1.4 nm/°C for the MZI and SPR, respectively. We demonstrate a sensitivity matrix used to simultaneously detect both parameters, solving the problem of temperature interference of RI variation-based biosensors. In addition, the sensor can also distinguish biological binding events by detecting the localized RI changes at the fiber’s surface. We realize label-free sensing of DNA hybridization detection by immobilizing probe DNA (pDNA) onto the fiber as the probe to capture complementary DNA (cDNA). The experimental results show that the sensor can qualitatively detect cDNA after temperature compensation, and the limit of detection (LOD) of the sensor reaches 80 nM. The proposed sensor has advantages of high sensitivity, real time, low cost, temperature compensation, and low detection limit and is suitable for in situ monitoring, high-precision sensing of DNA molecules, and other related fields, such as gene diagnosis, kinship judgment, environmental monitoring, and so on.

Refereed/Peer-reviewed A label-free DNA biosensor based on exposed core microstructured optical fiber for in-situ real-time DNA detection has been presented and experimentally demonstrated. The sensor is fabricated by splicing a section of tapered exposed core fiber (ECF) between two single-mode fibers (SMFs), forming a multimode Mach-Zehnder interferometer (MZI). The ECF design provides the evanescent field with the sensitivity of a micro/nano optical fiber. In this paper, the ECF has a large cladding diameter (160 µm) but a small core (9 µm), and the core of the ECF is further reduced by tapering, which significantly improves the refractive index (RI) sensitivity. The sensor can detect local RI changes that occur on the surface of the optical fiber due to the binding of biomolecules. We immobilized probe DNA (pDNA) on the exposed side of the core to detect the complementary DNA (cDNA), demonstrating use for specific and label-free sensing of DNA hybridization. Experimental results show that the sensor can qualitatively detect cDNA with the sensitivity of 0.0618 nm/nM and a detection limit of 0.31 nM at a temperature of 25 °C. The proposed DNA biosensor has potential applications in fast developing fields such as medical diagnostics, cancer screening, drug testing, and environmental engineering.

Highlights • Quantitative polymerase chain reaction (qPCR) in an all-fiber all-optical configuration. • Thermal cycling and fluorescence detection in a single optical-fiber-integrated device. • Advantages of small sample volume, portability and high-speed operation. • Potential ability to operate fluorescence-free through direct measurement of refractive index. • Target application is disease and pathogen detection in remote communities., Quantitative polymerase chain reaction (qPCR), the real-time amplification and measurement of a targeted DNA molecule, has revolutionized the biological sciences and is routinely applied in areas such as medical diagnostics, forensics, and agriculture. Despite widescale use of qPCR technology in the lab, the availability of low-cost and high-speed portable systems remains one of the barriers to routine in-field implementation. Here we propose and demonstrate a potential solution using a photonics-based qPCR system. By using an all-optical approach, we achieve ultra-fast temperature response with real-time temperature feedback using nanoliter scale reaction volumes. The system uses a microcavity to act as a nanoliter scale reaction vessel with a laser-driven and optically monitored temperature cycling system for ultrafast thermal cycling and incorporates an all-fiber fluorescence excitation/detection system to achieve real-time, high sensitivity fluorescence monitoring of the qPCR process. Further, we demonstrate the potential of the system to operate as a label-free qPCR system through direct optical measurement of the sample refractive index. Due to advantages in portability and fabrication simplicity, we anticipate that this platform technology will offer a new strategy for fundamental techniques in biochemistry applications, such as point-of-care and remote diagnostics.