Search

Your search keyword '"OST1 kinase"' showing total 2 results
Start Over Descriptor "OST1 kinase" Topic calcium
2 results on '"OST1 kinase"'

1. The calcium transporter ANNEXIN1 mediates cold-induced calcium signaling and freezing tolerance in plants.

Author

Liu Q, Ding Y, Shi Y, Ma L, Wang Y, Song C, Wilkins KA, Davies JM, Knight H, Knight MR, Gong Z, Guo Y, and Yang S

Subjects
Cell Membrane metabolism, Cold Temperature, Freezing, Gene Expression Regulation, Plant physiology, Protein Kinases metabolism, Transcription Factors metabolism, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Calcium metabolism, Calcium Signaling physiology, Cold-Shock Response physiology
Abstract

The transient elevation of cytosolic free calcium concentration ([Ca 2+ ] cyt ) induced by cold stress is a well-established phenomenon; however, the underlying mechanism remains elusive. Here, we report that the Ca 2+ -permeable transporter ANNEXIN1 (AtANN1) mediates cold-triggered Ca 2+ influx and freezing tolerance in Arabidopsis thaliana. The loss of function of AtANN1 substantially impaired freezing tolerance, reducing the cold-induced [Ca 2+ ] cyt increase and upregulation of the cold-responsive CBF and COR genes. Further analysis showed that the OST1/SnRK2.6 kinase interacted with and phosphorylated AtANN1, which consequently enhanced its Ca 2+ transport activity, thereby potentiating Ca 2+ signaling. Consistent with these results and freezing sensitivity of ost1 mutants, the cold-induced [Ca 2+ ] cyt elevation in the ost1-3 mutant was reduced. Genetic analysis indicated that AtANN1 acts downstream of OST1 in responses to cold stress. Our data thus uncover a cascade linking OST1-AtANN1 to cold-induced Ca 2+ signal generation, which activates the cold response and consequently enhances freezing tolerance in Arabidopsis., (© 2020 The Authors.)

Published
2021
Full Text
View/download PDF

2. The calcium transporter ANNEXIN1 mediates cold-induced calcium signaling and freezing tolerance in plants

Author

Zhizhong Gong, Yiting Shi, Yanglin Ding, Julia M. Davies, Marc R. Knight, Chun-Peng Song, Katie A. Wilkins, Shuhua Yang, Liang Ma, Yi Wang, Qiangbo Liu, Yan Guo, Heather Knight, Wang, Yi [0000-0002-3660-5859], Wilkins, Katie A [0000-0001-6513-856X], Yang, Shuhua [0000-0003-1229-7166], and Apollo - University of Cambridge Repository

Subjects
Mutant, Arabidopsis, chemistry.chemical_element, Biology, Calcium, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Gene Expression Regulation, Plant, calcium-permeable transporter AtANN1, Freezing, Arabidopsis thaliana, Calcium Signaling, Molecular Biology, 030304 developmental biology, Calcium signaling, 0303 health sciences, General Immunology and Microbiology, Arabidopsis Proteins, General Neuroscience, Cold-Shock Response, Cell Membrane, calcium signal, Transporter, Articles, biology.organism_classification, freezing tolerance, Cell biology, Cold Temperature, chemistry, Phosphorylation, OST1 kinase, Protein Kinases, 030217 neurology & neurosurgery, Transcription Factors
Abstract

The transient elevation of cytosolic free calcium concentration ([Ca(2+)](cyt)) induced by cold stress is a well‐established phenomenon; however, the underlying mechanism remains elusive. Here, we report that the Ca(2+)‐permeable transporter ANNEXIN1 (AtANN1) mediates cold‐triggered Ca(2+) influx and freezing tolerance in Arabidopsis thaliana. The loss of function of AtANN1 substantially impaired freezing tolerance, reducing the cold‐induced [Ca(2+)](cyt) increase and upregulation of the cold‐responsive CBF and COR genes. Further analysis showed that the OST1/SnRK2.6 kinase interacted with and phosphorylated AtANN1, which consequently enhanced its Ca(2+) transport activity, thereby potentiating Ca(2+) signaling. Consistent with these results and freezing sensitivity of ost1 mutants, the cold‐induced [Ca(2+)](cyt) elevation in the ost1‐3 mutant was reduced. Genetic analysis indicated that AtANN1 acts downstream of OST1 in responses to cold stress. Our data thus uncover a cascade linking OST1‐AtANN1 to cold‐induced Ca(2+) signal generation, which activates the cold response and consequently enhances freezing tolerance in Arabidopsis.

Published
2020

Catalog

Books, media, physical & digital resources
See catalog results