1. Gold nanoparticles potentiate Ca V channel currents in rat tail artery myocytes.
- Author
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Fusi F, Sgaragli G, and Valoti M
- Subjects
- Animals, Arteries cytology, Arteries metabolism, Barium metabolism, Calcium Channels chemistry, Calcium Channels, L-Type chemistry, Cells, Cultured, Environmental Pollutants chemistry, Gold chemistry, Male, Membrane Potentials, Metal Nanoparticles chemistry, Muscle, Smooth, Vascular cytology, Muscle, Smooth, Vascular metabolism, Particle Size, Patch-Clamp Techniques, Povidone chemistry, Povidone toxicity, Rats, Wistar, Single-Cell Analysis, Surface Properties, Tail, Toxicity Tests, Acute, Arteries drug effects, Calcium Channels metabolism, Calcium Channels, L-Type metabolism, Environmental Pollutants toxicity, Gold toxicity, Metal Nanoparticles toxicity, Muscle, Smooth, Vascular drug effects
- Abstract
This study was designed to unveil effects of 5-nm sized, polyvinylpyrrolidone-coated gold nanoparticles (AuNPs) on vascular Ca
V 1.2 and CaV 3.1 channels. Ba2+ currents through both channels (IBa1.2 and IBa3.1 ) were recorded in single myocytes isolated from the rat tail main artery by means of the whole-cell patch-clamp method. AuNPs increased IBa1.2 and IBa3.1 amplitude in a concentration- and Vh -dependent manner. Neither the voltage dependence of inactivation and activation curves nor inactivation and activation kinetics were affected by AuNPs. In conclusion, these findings warrant further investigation to clarify whether different types of NPs possess the same stimulatory activity and may represent a toxic hazard to humans., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2018
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