1. Development of a synbiotic that protects against ovariectomy-induced trabecular bone loss.
- Author
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Lawenius L, Gustafsson KL, Wu J, Nilsson KH, Movérare-Skrtic S, Schott EM, Soto-Girón MJ, Toledo GV, Sjögren K, and Ohlsson C
- Subjects
- Amino Acids, Branched-Chain, Animals, Bacteria, Fatty Acids, Volatile, Female, Humans, Mice, Ovariectomy, Vitamin K 2, Cancellous Bone, Synbiotics
- Abstract
The gut microbiome has the capacity to regulate bone mass. The aim of this study was to develop a nutritional synbiotic dietary assemblage at an optimal dose to maintain bone mass in ovariectomized (Ovx) mice. We performed genomic analyses and in vitro experiments in a large collection of bacterial and fungal strains (>4,000) derived from fresh fruit and vegetables to identify candidates with the synergistic capacity to produce bone-protective short-chain fatty acids (SCFA) and vitamin K2. The candidate SBD111-A, composed of Lactiplantibacillus plantarum , Levilactobacillus brevis , Leuconostoc mesenteroides , Pseudomonas fluorescens , and Pichia kudriavzevii together with prebiotic dietary fibers, produced high levels of SCFA in vitro and protected against Ovx-induced trabecular bone loss in a dose-dependent manner in mice. Metagenomic sequencing revealed that SBD111-A changed the taxonomic composition and enriched specific pathways for synthesis of bone-protective SCFA, vitamin K2, and branched-chain amino acids in the gut microbiome. NEW & NOTEWORTHY We performed genomic analyses and in vitro experiments in a collection of bacterial and fungal strains. We identified a combination (SBD111-A) that produced high levels of SCFA in vitro and protected against ovariectomy-induced bone loss in a dose-dependent manner in mice. Metagenomic sequencing revealed that SBD111-A changed the taxonomic composition and function of the gut microbiome and enriched pathways for synthesis of bone-protective SCFA, vitamin K2, and branched-chain amino acids.
- Published
- 2022
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